Tài liệu Báo cáo Y học: NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3 pptx
Tài liệu Báo cáo Y học: A novel, inducible, citral lyase purified from spores of Penicillium digitatum docx
... as the standard.RESULTSInduction of citral lyaseInitially, the reproducibility of the results was hindered byvariations in the citral lyase activity of the P. digitatumspores. Remarkably, ... hydratase and aldolase activityin a single enzyme. No other enzyme has been reported to catalyse the conversion of citral into methylheptenone and acetaldehyde, or a similar conversion of othera,b-unsaturated ... is approximately the monomeric size of these hydratase/aldolase enzymes. All of these enzymesexhibit more then 75% of their maximum activity at pH 7.6, the optimum pH of citral lyase [27,30–32]....
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Tài liệu Báo cáo Y học: Systematic search for zinc-binding proteins in Escherichia coli potx
... the Zn(II) -binding activity and to estimate the Zn(II) -binding affinity for the newly identified Zn(II)- binding proteins, we next cloned the genes encoding some of the newly identified Zn(II) -binding ... influence of the His6tag on the Zn(II) -binding activity was then tested. The His6tag added to the test proteins was found to show a lowlevel of the Zn(II) -binding activity, but the level of 65Zn(II)-blotting ... those of the reference proteins, Def, MerR and Fur.Specificity of Zn(II) binding by the whole cell extracts To examine the specificity of Zn(II) binding for the known and novel E. coli Zn(II)-binding...
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Tài liệu Báo cáo Y học: NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3 pptx
... NMR of MUC-1 glycopeptides (Eur. J. Biochem. 269) 1455 NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective ... enhances binding affinity of glycopeptides to the antibody [18].Conventional pepscan analysis however, does not alloweasy analysis of the contribution of the carbohydrateportion. To assess the involvement ... he binding pocket of the mAb SM3 very well.Keywords: glycopeptide antibody complex; STD N MR; breast cancer; MUC-1; binding epitope. The extracellular part of the epithelial glycoprotein MUC-1 consists...
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Tài liệu Báo cáo khoa học: Structure determination and biochemical studies on Bacillus stearothermophilus E53Q serine hydroxymethyltransferase and its complexes provide insights on function and enzyme memory doc
... interactionwith the phenolate oxygen of Y5 1, N of G257 and NE2 of Q53 of the symmetry related subunit. Anotheroxygen atom is held by hydrogen bonding to NE2 of H122 and phenolate oxygen of Y5 1 of the symmetryrelated ... breakdown of the two-fold symmetry and hence to the monoclinic crystalform [12]. Attempts to crystallize E53QbsSHMT in the presence of Gly and FTHF yielded orthorhombic crys-tals and the density for ... mgÆmL)1protein in the presence of l-Ser,Gly, THF and FTHF.Stopped-flow spectrophotometry The presteady state kinetics of wild-type and the mutantenzymes with Gly or Gly and THF ⁄ FTHF were monitoredusing...
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
... The phosphoryl and pyridoxyl moieties of the P-pyridoxyl group are shown in red and magenta, respectively. The fractional solventexposed area of the P-pyridoxyl group is 0.39 and 0.082 for the ... 2002 conformational changes caused by the ligand are easilysensed by the P-pyridoxyl chromophore. Figure 4 shows the modeled structure of the P-pyridoxyl-PEP carboxykinase in the free and substrate ... adduct of PEP carboxykinase exhibits a band with a maximum at326 nm due to pyridoxyl moiety and a band at 280 nmcorresponding to the aromatic amino acids of the protein. The fluorescence of the...
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Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx
... G. (1986) The oxidation of primary trimethylsilyl ethers to aldehydes: a selective conversion of a primary hydroxy group intoan aldehyde group in the presence of a secondary hydroxy group.J. ... wererecently introduced for the preparation of C-6 aldehydederivatives of Glc [15]. The key step is the use of Collinsreagent for the selective oxidation of the primary trimethyl-silyl ether of the ... inhibitor of Glc and 3FGlc phosphorylation by EIIGlc[15]. The newly synthesizedC-1 epoxide 3a is the first and so far only analogue thatpreferentially binds to the Glc high-affinity site of EIIGlc .The Kmand...
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Tài liệu Báo cáo Y học: Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA pdf
... novo methylation of DNA[15]. Interestingly, the de novo activity of Dnmt1 isstimulated by binding of methylated DNA to an allostericsite located in the N-terminal domain of the enzyme[18,19]. ... development and evenin adult cells to silence acquired proviral DNA or to alter the developmental program of the cell. Aberrant de novomethylation may lead to hypermethylation of promotorregions of ... [6,7]. The methylationpattern is created by de novo methylation and demethyla-tion of the DNA, and maintained during mitosis bymaintenance methylation [reviews 8,9]. De novo methyla-tion of DNA...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... 2 h and 20 h to determine the substratespecificity of the enzyme. The degradation of the polymer and subsequent oligosaccharide formation was analyzed byHPAEC and MALDI-TOF MS. HPAEC analysis ... potato resulting in a 30% reduction of the galactosylcontent of the pectin fraction of the cell walls [17].In this paper we describe the cloning, characterization and expression analysis of the ... duplicate. The hydrolysis activity of GALA with different arabino-galactans was measured in duplicate by HPAEC and MALDI-TOF MS after incubation of 1 mgÆmL)1 of potato, onion and soy arabinogalactan...
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... governed byone or even two residues, but is most likely accomplished by the concerted effects of a combination of key residues.Further analysis of these and other mutant SODs iscurrently underway.ACKNOWLEDGEMENTSWe ... conclusiveresult.Effect of hydrogen peroxide on enzyme activityHydrogen peroxide, the product of the SOD reaction, isan inhibitor of SODs and an inactivator of FeSOD. It isoften used to distinguish between the ... above.Assay for superoxide dismutase activity The specific activity of SOD was measured spectrophoto-metrically by its inhibitory action on the superoxide-dependent reduction of cytochrome c by xanthine-xanthineoxidase...
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Tài liệu Báo cáo Y học: The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168 pdf
... thispossibility. Alternatively, this enzyme may be involved in the epimerization reaction necessary for the biosynthesis of the capsular heptopyranose as alditol acetate analysis (results not shown) and ... characterization of specific enzymes thatare involved in the biosynthesis of these structures and maylead to the discovery of potential therapeutic targets. Inaddition, the correlation of carbohydrate ... established by NMR was observed in the FAB-MS and MALDI-MS (Fig. 8) of the methylated PS-2. The MALDI-MS spectra shows a series of A-type primaryglycosyl oxonium ions and secondary ions of definedcompositions...
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Từ khóa: tài liệu báo cáo khoa học bản chất của khủng hoảng kinh tế thế giới pdftài liệu báo cáo nghiên cứu khoa họctai lieu bao cao thuc tap y si da khoatai lieu bao cao thuc tap tim hieu nhan cach mot hoc sinhbáo cáo y họctài liệu báo cáotài liệu báo cáo môn triếttài liệu báo cáo tài chínhtài liệu di truyền y họctài liệu báo cáo mônbáo cáo y họctài liệu báo cáo tài chính vốn bằng tiền tai doanh nghiệptài liệu vi sinh y họctài liệu báo cáo môn triếtquan hệ sản xuấttài liệu báo cáo thuếNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinChuong 2 nhận dạng rui roKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt nam