Tài liệu Báo cáo Y học: Induction of (2¢)5¢)oligoadenylate synthetase in the marine sponges Suberites domuncula and Geodia cydonium by the bacterial endotoxin lipopolysaccharide docx

... Biochem. 269) Ó FEBS 2002 Induction of (2¢)5¢)oligoadenylate synthetase in the marine sponges Suberites domuncula and Geodia cydonium by the bacterial endotoxin lipopolysaccharide Vladislav A. ... to24 h . The i dentity of the p3A2product synthesized by the (2–5)A synthetase both in tissue and in primmorphs of S. domuncula was veriﬁed by TLC and HPLC analysis astriphosphorylated and/ or ... activated by certain classes of RNA, mainly double-stranded RNA [14]. In vertebrates the (2–5)A pathway is also induced by interferons [15]. The major enzyme in this pathway, the (2–5)A synthetase...

Tài liệu Báo cáo Y học: Prediction of protein–protein interaction sites in heterocomplexes with neural networks ppt

... is involved in many proteinfolding and trafﬁc processes i n the cell. The main compo-nent of the system is DnaK, a t wo-domain protein with aC-terminal domain responsible for the binding of ... unfoldedhydrophobic peptides and a N-terminal domain, whichbinds ATP. This protein can bind and release peptides (in the Ct domain) in a cycle driven by nucleotide hydrolysis and exchange (in the ... solving the structure of some of the complexes or by other experimental means.CONCLUSIONSWe have analysed the possibility of predicting the residuesforming part of protein–protein interacting...

Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx

... identiﬁed,including CYP1A1, human keratin 4, intestinal alkalinephosphatase, ornithine decarboxylase, histidine decar-boxylase and cyclin D1 [17]. KLF4 regulates the targetgenes by binding to the ... reporter assay. The results showed that induction of KLF4 by high-density lipoproteins could promote the expres-sion of SR-BI, resulting from the binding to putative KLF4 bindingelement on the promoter ... identiﬁed in the epithelial lining of the gut and skin, and subse-quent studies have shown it to play a role in the regu-lation of cellular growth and differentiation in thesetissues [1]. Recently,...

Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx

... progression, the understanding of these underly-ing intracellular events is of importance. In the presentstudy, we explored the signaling pathway linking disrup-tion of E-cadherin-dependent ... component of the cell–cell adhesion complex and required for the for-mation of epithelia in the embryo and the maintenance of the polarized epithelial structure in the adult [2]. Asa single-span ... assemble the cytoplasmic cell adhesioncomplex (CCC) on its intracellular domain and linkE-cadherin indirectly to the actin cytoskeleton [3].Through the establishment of the CCC, the initialinteraction...

Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf

... between FeSOD and MnSOD [7]. The position of the glutamine residue, which isstructurally equivalent in the enzymes, is contributed by the N domain of FeSODs (Q69) and the C domain of MnSODs(Q146). ... wild-type enzymes, the metal sites of the puriﬁed proteins are either completely(FeSOD, 1.17) or nearly fully occupied by the metal ionfrom which the SOD derives, and very little of the contrarymetal ... combination of key residues.Further analysis of these and other mutant SODs iscurrently underway.ACKNOWLEDGEMENTSWe are indebted to G. Peplow, F. Yamakura and T. Matsumoto for the analyses of iron and...

Tài liệu Báo cáo Y học: Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species pptx

... appearance of the ThDP adduct with the substratemoiety during the complex-catalyzed succinyl-CoA hydro-lysis [72] accounts for the inactivation by dihydrolipoamide in the presence of succinyl-CoA ... network of interacting lipoyl moieties of the E2 coreprovides a good explanation for these phenomena.Considering possible mechanisms of the inactivation of the complex by the thiyl radical of dihydrolipoate, ... with the ferricyanide assay due to the many-fold dilution of the preincubated mixture upon assay.RESULTSTrapping of radical species in the course of reactionscatalyzed by the 2-oxo acid dehydrogenase...

Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx

... performed by using different input order of the sequences. Each analysisinvolved two steps: stepwise addition and nearest neighborinterchanges. The most likely tree was obtained by using the test of ... hydrophobicligands, binding to bilirubin and carcinogens [3,4], the isomerization of maleylacetoacetate and the regulation of stress kinases and apoptosis [5,6].Based on their sequence structure, catalytic ... agarose. In all cases, protein purity wasdetermined by SDS/PAGE and protein concentration wasmeasured by bicinchoninic acid method following manu-facturers indications (Sigma). Antiserum against the...

Tài liệu Báo cáo Y học: Oxidation of phenols by laccase and laccase-mediator systems doc

... bis-hydroxymethylated. The purity of these compounds (> 96%) was checked by 1HNMR and HPLC.Spectrophotometric determination of laccase activity The activity of laccase was determined by following the rate of ... N-hydroxyphtalimide (HPI) and 2,2,6,6-tetramethylpiperidin-1-yloxy free radical (TEMPO) asmediators, since they greatly differ both in their mechan-ism of action and in their speciﬁcity. In fact, HPI isrepresentative ... consump-tion. On the other hand, the laccase/TEMPO systemperforms the selective oxidation of the hydroxymethylgroups of the substrate to aldehydes. Both with laccasealone and with the laccase/HPI system,...

Tài liệu Báo cáo Y học: Characterization of a cloned subtilisin-like serine proteinase from a psychrotrophic Vibrio species doc

... in psychrophilic enzymes. Glycine residues are highly con-served in the sequences of the proteinases compared in this study. For example, of the 37 glycines present in the proteinase domain of ... domain (see Fig. 2 or 3). The proteinase domain of VPR additionally contains Cys277 and Cys281, and Cys351 and Cys362 in the C-terminal domain, that suppo-sedly also form disulﬁde bonds as the ... conserved in all theseenzymes, and except for PRK they apparently all containidentical disulﬁde bonds, i.e. Cys67–Cys99 and Cys163–Cys194 (numbering of VPR from the N-terminal of the proteinase...

Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt

... maintenance of tumor cell growth[3]. The ﬁnding that the ERK signaling pathway is involved in tumor cell migration and invasion [4,5], as well as in tubular formation induced by insulin in human endothelialcells ... NAMI-A-mediated inhibition of ERK1/2 phosphorylation may be lying upstream of ERK1/2.Using a serine/threonine kinase SPA assay kit, the capability of NAMI-A to affect the ERK1/2 phosphoryla-tion activity ... as aninternal control for mRNA integrity and equal loading. The levels of radioactivity incorporated into c-myc product werenormalized by comparison with the levels of radioactivityincorporated...

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