... Peptide
Synthesizer (Applied Biosystems, Foster City, CA, USA) as
described previously [51]. The homogeneity of the synthetic
peptides preparation was assessed by MALDI-TOF MS
and analytical RP-HPLC.
Decapeptides ... 481–485.
10 Ollivaux C & Soyez D (2000) Dynamics of biosynthe-
sis and release of crustacean hyperglycemic hormone
isoforms in the X-organ-sinus gland complex of th...
... relationships of human aromatase (Eur. J. Biochem. 269) 1405
Study of substrate specificity of human aromatase by site directed
mutagenesis
P. Auvray
1,
*
,†
, C. Nativelle
1,†
, R. Bureau
2
, P. Dallemagne
2
, ... mechanism of aromatase cytochrome
P450. A site- directed mutagenesis study. Eur. J. Biochem. 268,
243–251.
63. Gaillard, J.L. (1991) Equine tes...
... Rafferty
3
, Robert G. Lloyd
2
,
Jane A. Grasby
4
and David P. Hornby
1
1
Transgenomic Research Laboratory, Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield,
UK;
2
Institute ... over
only a 10-fold increase in protein concentration, nearly
100% of the binding sites are occupied by RuvA. The
concentration at which approximately 50% of the DNA...
... the cystatin F produced by U937 cells is
retained intracellularly or is taken up by the cells immedi-
ately following secretion. Accordingly, the relatively low
amount of cystatin F produced by ... to detect cystatin F directly
in culture media or cell lysates by immunoblotting. How-
ever, by absorption on Sepharose-bound Cm-papain fol-
lowed by Western blot analysis, the cell l...
... DISCUSSION
By site- directed mutagenesis, a D153N mutant form of
E. coli agmatinase was obtained. The mutant enzyme
retained about 5% of wild-type activity and it was equally
active when assayed in ... (Asp153), may be reason-
ably expected. This expectation is supported by our present
findings of a markedly decreased activity of a D153N
variant of E. coli agmatinase. From th...
... Wajant
1
1
Institute of Cell Biology and Immunology and
2
Institute of Industrial Genetics, University of Stuttgart, Germany;
3
Institute of
Immunology, Christian-Albrechts-University of Kiel, Germany
To gain ... 8).
Correspondingly, fluorescence of cytoplasmic T4(259–470)–
YFP and full-length T4–GFP was already significantly
reduced after 2 min of bleaching in a small area of...
... a cyclic diphosphate by the
consecutive action of 4-diphosphocytidyl-2C-methyl-
D-
erythritol synthase, 4-diphosphocytidyl-2C-methyl-
D-erythritol
kinase and 2C-methyl-
D-erythritol 2,4-cyclodiphosphate
synthase ... that
[1-
3
H]2C-methyl-D-erythritol 4-phosphate was diverted effici-
ently to the 2C-methyl-
D-erythritol 2,4-cyclodiphosphate
Fig. 3.
13
C NMR signals of 2C-methyl-D-erythri...
... phosphorylation of the C-terminus of
R* and binding by arrestin. Phosphorylation somewhat
decreases the ability of R* to signal transducin. Rapid shut-
off of R* signalling is then accomplished by ... membrane hyper-
polarization and the sensation of light (reviewed in [1]). The
sensitivity of the system requires that the signal transmitted
by R* be rapidly attenuated. This...
... expression of highly speci-
fic proteases that proteolytically activate the PDGFs
will also influence the availability and activity of the dif-
ferent isoforms. This can be exemplified by the proteo-
lytic ... interstitial cells in a diversity of renal diseases but
not in normal human kidneys. Most interestingly,
PDGFR-a is extensively expressed by interstital cells of
normal kidne...
... with
replacement of this segment by the rPDE2 region,
which comprised only 23% of the residues of the
cyaB1 GAF B domain, was exclusively, yet weakly, sti-
mulated by cGMP, whereas the activation by cAMP
was ... lm, similar to wild-type cyaB1, and, surpris-
ingly, activation by cAMP was eliminated (Fig. 2A,
Table 1). Obviously, exchange of this region switched
the specificity...