Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx
Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx
... 2002 Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low ... B.W. (1993) Enzymatic hydro-lysis of whey proteins. In uence of heat treatment of alpha-lac-talbumin and beta-lactoglobulin on their proteolysis by pepsin and papain. Neth. Milk Dairy J. 47, 15–22.18. ... of these hydrophobic residues are pointinginwards in the native protein structure, in which they line the hydrophobic cavity t hat characterizes all lipocalins. The insensitivity to chymotryptic...
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Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc
... proteasecathepsin D, as indicated by the following observa-tions: (a) the inhibitor profile of the endosomalETA-degrading activity was very similar to that of cathepsins B and D [30]; and (b) the ... determined. Finally, we assignan important role to the endosomal acidic cathep-sins B and D in increasing the in vitro transfer of the ADP-ribosyl moiety of NAD+to EF-2 by ETA,but not in the ... assay, intact ETA inducedADP-ribosylation of EF-2 and mitochondrial release of cytochrome c, with the former effect being efficiently increased by a cathepsin B ⁄ cathepsin Dpretreatment. The...
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... visualized by SOD activity staining may havebeen derived from further interactions of the fusion protein GST domains (Fig. 2B). Although not visualized in thesezymograms, all of the fusion proteins ... combination of key residues.Further analysis of these and other mutant SODs iscurrently underway.ACKNOWLEDGEMENTSWe are indebted to G. Peplow, F. Yamakura and T. Matsumoto for the analyses of iron and ... between FeSOD and MnSOD [7]. The position of the glutamine residue, which isstructurally equivalent in the enzymes, is contributed by the N domain of FeSODs (Q69) and the C domain of MnSODs(Q146)....
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Tài liệu Báo cáo Y học: Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species pptx
... appearance of the ThDP adduct with the substratemoiety during the complex-catalyzed succinyl-CoA hydro-lysis [72] accounts for the inactivation by dihydrolipoamide in the presence of succinyl-CoA ... several lines of evidencepoint to the presence of the thiyl radical in the aerobicsystem. First, the complexes are inactivated by 2-oxo acid and CoA in the presence of O2(Fig. 2A) and the inactivation ... rotation inherent in the nitroxides being localizedto the protein interior. Indeed, EPR spectra of the PBNadducts with the protein cysteine residues [66,67] as well as the spectrum obtained in our...
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Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx
... model the C-terminal domain of SmGSTOdue to the low rate of identity between the two proteins in this region. However, as the active sites of the GSTO1-1 arelocated at the N-terminal domain, ... are in line with the high GSH Kmvalue obtained forSmGSTO and the strong inhibitory effect of S-hexylglutathione on the enzyme activity. The preference of SmGSTO for more hydrophobic alkyl-bound ... microliters of the original protein, binding assaysupernatant and eluate were analyzed by SDS/PAGE.Expression of SmGSTO along the parasite life cycleTotal proteins were prepared as follows: S....
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Tài liệu Báo cáo Y học: Oxidation of phenols by laccase and laccase-mediator systems doc
... consump-tion. On the other hand, the laccase/TEMPO systemperforms the selective oxidation of the hydroxymethylgroups of the substrate to aldehydes. Both with laccasealone and with the laccase/HPI system, ... promotes the formation of the same phenoxy radicalintermediate as laccase, yields the same products as the enzyme alone, namely, ring-opening and phenol couplingproducts, with a comparable extent of ... and 4-tert-butylphenol being bis-hydroxymethylated. The purity of these compounds (> 96%) was checked by1HNMR and HPLC.Spectrophotometric determination of laccase activity The activity of laccase...
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Tài liệu Báo cáo Y học: Characterization of a cloned subtilisin-like serine proteinase from a psychrotrophic Vibrio species doc
... N-terminal of the proteinase domain (see Fig. 2 or 3). The proteinase domain of VPR additionally contains Cys277 and Cys281, and Cys351 and Cys362 in the C-terminal domain, that suppo-sedly also ... highhomology to proteinases of the proteinase K family of subtilisin-like proteinases. With respect to the enzymecharacteristics compared in this study the properties of the wild-type and recombinant proteinases ... in psychrophilic enzymes. Glycine residues are highly con-served in the sequences of the proteinases compared in this study. For example, of the 37 glycines present in the proteinase domain of...
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Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt
... NAMI-A-mediated inhibition of ERK1/2 phosphorylation may be lying upstream of ERK1/2.Using a serine/threonine kinase SPA assay kit, the capability of NAMI-A to affect the ERK1/2 phosphoryla-tion activity ... as aninternal control for mRNA integrity and equal loading. The levels of radioactivity incorporated into c-myc product werenormalized by comparison with the levels of radioactivityincorporated ... maintenance of tumor cell growth[3]. The finding that the ERK signaling pathway is involved in tumor cell migration and invasion [4,5], as well as in tubular formation induced by insulin in human endothelialcells...
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Tài liệu Báo cáo Y học: Role of electrostatics in the interaction between plastocyanin and photosystem I of the cyanobacterium Phormidium laminosum ppt
... mutatednegatively and positively charged residues on the proposedinteraction site of plastocyanin with cytochrome f and analysed the reaction of these mutants with the solubleredox-active domain of cytochrome ... Further-more, the type I copper protein plastocyanin can bereplaced by cytochrome c6, a redox protein of similar sizebut entirely different folding, in a number of eukaryoticalgae and cyanobacteria ... Forwild-type plastocyanin, the rate increased with increasingsalt concentration, as observed by Balme et al.[7].Thisisinclear contrast with the reaction of wild-type plastocyanin with Cyt f, where the...
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Tài liệu Báo cáo Y học: Inhibition of nuclear pre-mRNA splicing by antibiotics in vitro pot
... aminoglycoside,kanamycin, the a minocyclitol glycoside, streptomycin, t hepenicillins, ampicillin and penicillin G, the aminocyclitol,spectinomycin, tetracycline and Cl-tetracycline, and the macrolide, erythromycin) ... atacon®rmed the possible binding of Cl-tetracycline a ndstreptomycin to the pre-mRNA. With Cl-tetracycline and s treptomycin, the preincubation resulted in a 65% and 46% increase in splicing i nhibition, ... with the binding o f the functional sub strate.Type-II binding sites are the central metal ion-binding pock-ets i n the catalytic cores of ribozymes. In type-II bindingsites the aminoglycosides...
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Từ khóa: tài liệu báo cáo khoa học bản chất của khủng hoảng kinh tế thế giới pdftài liệu báo cáo nghiên cứu khoa họctai lieu bao cao thuc tap y si da khoatai lieu bao cao thuc tap tim hieu nhan cach mot hoc sinhbáo cáo y họctài liệu báo cáoNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếThơ nôm tứ tuyệt trào phúng hồ xuân hươngTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMMÔN TRUYỀN THÔNG MARKETING TÍCH HỢP