Tài liệu Báo cáo Y học: Prediction of protein–protein interaction sites in heterocomplexes with neural networks ppt
Tài liệu Báo cáo Y học: Prediction of protein–protein interaction sites in heterocomplexes with neural networks ppt
... many proteinfolding and traffic processes i n the cell. The main compo-nent of the system is DnaK, a t wo-domain protein with aC-terminal domain responsible for the binding of unfoldedhydrophobic ... N-terminal domain, whichbinds ATP. This protein can bind and release peptides (in the Ct domain) in a cycle driven by nucleotide hydrolysisand exchange (in the Nt domain). The structures of both0.70.750.80.850.90.9510 ... mutations, by solving the structure of some of thecomplexes or by other experimental means.CONCLUSIONSWe have analysed the possibility of predicting the residuesforming part of protein–protein interacting...
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Tài liệu Báo cáo Y học: Induction of (2¢)5¢)oligoadenylate synthetase in the marine sponges Suberites domuncula and Geodia cydonium by the bacterial endotoxin lipopolysaccharide docx
... (2–5)Asynthesizing activity in S. domuncula.Despite the initially high (2–5)A synthesizing activity in tissue of G. cydonium (aquarium animals), the incubation with LPS caused significant increase of ... he only species with (2–5)Asynthesizing activity within the p hylum Porifera. We haveidentified (2–5)A synthesizing activity in different marinesponges, i ncluding S. domuncula (this study and ... [14]. In vertebrates the (2–5)A pathway is also induced byinterferons [15]. The major enzyme in this pathway, the(2–5)A synthetase catalyzes the synthesis of a series of 2¢)5¢-linked oligoadenylates,...
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Tài liệu Báo cáo Y học: Analyses of the CYP11B gene family in the guinea pig suggest the existence of a primordial CYP11B gene with aldosterone synthase activity docx
... decrease in the activityfor the 11b-hydroxylase, CYP11B1. Intriguingly, however,the 11b-hydroxylase activity of the aldosterone synthaseCYP11B2 was basically unaffected whereas the 18-hydroxy-lation ... major mineralocorticoid by means of an 11b-hydroxylation and an 18-hydroxylation/oxidation of 11-deoxycorticosterone. Thus, the proteins of the CYP11Bsubfamily catalysing the last biosynthetic ... remaining introns. Finally,the 3¢ UTRs of the guinea pig CYP11B genes show only42% similarity whereas the 3¢UTR of the guinea pigCYP11B2 gene shares up to 72% identity with thecorresponding...
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
... properties. In this work we took advantage of the specific labeling with PLP of Lys288 in E. coli PEP carboxykinase to gaininsight into conformational alterations of the protein uponligand binding in ... domain movements in the protein. In thiswork, the interaction of several substrates and ligands with E. coli PEP carboxykinase has been studied in the phos-phopyridoxyl (P-pyridoxyl)-enzyme ... estimation of the binding affinity of PEPfor E. coli PEP carboxykinase was obtained from thequenching of the emission of Trp residues of the unlabeledprotein. When the protein was titrated with PEP in...
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... constitute aclass of structurally equivalent metalloenzymes prevalent in prokaryotes and in eukaryotic mitochondria, respectively.They exhibit a very high degree of homology in bothsequence ... manganese.Selectivity of the proteins for their metal cofactor has beendemonstrated in vivo [3] and although apoenzymes of eachtype of SOD may be reconstituted by the addition of metals,the resulting enzyme ... active site of the enzyme byreducing the occupied internal volume, allowing the intro-duction of an extra water molecule [29]. Replacement of thesame residue by Ala similarly maintains the same...
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Tài liệu Báo cáo Y học: Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species pptx
... appearance of the ThDP adduct with the substratemoiety during the complex-catalyzed succinyl-CoA hydro-lysis [72] accounts for the inactivation by dihydrolipoamide in the presence of succinyl-CoA ... thethiyl radical of the lipoyl residue should efficiently interact with the E1 catalytic intermediate, as the lipoyl-bearingdomain of E2 is designed for this interaction (reaction 2). In this ... 11 of linoleic acid 1.62–1.63 0.26–0.3 Stable 82, 83 12Thiyl (RS•) of DTT of glutathione of cysteine of protein Cys in 5Murea1.561.561.56–1.571.60.320.320.34–0.350.36minminmin...
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Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx
... resin was washed four times with 250 lLof50 mMHepes, pH 8.0, and eluted by adding 10 lLofthesame buffer containing 5 mMGSH, GSSG or S-hexyl GSH.Ten microliters of the original protein, binding ... are in line with the high GSH Kmvalue obtained forSmGSTO and the strong inhibitory effect of S-hexylglutathione on the enzyme activity. The preference of SmGSTO for more hydrophobic alkyl-bound ... expressedmore in parasitic stages than in free living stages during theS. mansoni life cycle.Characterization of recombinant SmGSTO enzymaticactivityRecombinant GSTO was used to assay its enzymaticactivity....
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Tài liệu Báo cáo Y học: Oxidation of phenols by laccase and laccase-mediator systems doc
... 2,2,6,6-tetramethylpiperidin-1-yloxy free radical (TEMPO) asmediators, since they greatly differ both in their mechan-ism of action and in their specificity. In fact, HPI isrepresentative of a mediator ... activityThe activity of laccase was determined by following the rate of oxidation of 2,2¢-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) to ABTS radical cation (ABTS•+), byplotting the ... role of the enzyme, with the onlydifference of presenting fewer stringent steric and solubilityrequirements. The phenoxy radical of the product, in turn,should evolve towards end-products with...
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Tài liệu Báo cáo Y học: Characterization of a cloned subtilisin-like serine proteinase from a psychrotrophic Vibrio species doc
... identical inactivation behaviour, indicating thatthe same disulfide(s) are involved in maintaining theintegrity of active protein structure in both wild-type andrecombinant VPR.The wild-type and ... According to ourhomology model only one of the three extra glycines in the cold-adapted proteinase is located in a loop region of the protein. Thus an increased number of ÔflexibleÕglycines in ... in psychrophilic enzymes. Glycine residues are highly con-served in the sequences of the proteinases compared in this study. For example, of the 37 glycines present in theproteinase domain of AQUI, 29 are...
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Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt
... as aninternal control for mRNA integrity and equal loading. Thelevels of radioactivity incorporated into c-myc product werenormalized by comparison with the levels of radioactivityincorporated ... inhibition of ERK1/2 phosphorylation may be lying upstream of ERK1/2.Using a serine/threonine kinase SPA assay kit, thecapability of NAMI-A to affect the ERK1/2 phosphoryla-tion activity was also assessed. ... belying upstream of ERK1/2.Recent observations indicate that activation of Raf byPMA may trigger the same signaling pathway as oncogenicRaf, or Raf activation by Ras in combination with tyrosinephosphorylation...
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