... [5–8] as effectors to control the catalytic activity of the DNAymes; engineering DNA-zyme-based sensors for Mg 2+ [9,10], Cu 2+ [11], Hg 2+ [ 12, 13], Pb 2+ [14], and UO 2 2+[15]; and constructingmolecular ... Other metal ions, includingMg 2+ ,Ca 2+ ,Mn 2+ ,Co 2+ ,Ni 2+ ,Cd 2+ ,Zn 2+ , and Ba 2+ , failed to facilitate the cleavage activity. The reconstructed DNAzyme was shown to use divalentcopper ions ... al. 25 44 FEBS Journal 27 7 (20 10) 25 43 25 49 ª 20 10 The Authors Journal compilation ª 20 10 FEBSbeing reached at 100 lm. The rate of DNA cleavagewas highly dependent on the concentration of Cu 2+ used...