Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx
... The molecular surface of proteolytic enzymes has an important role
in stability of the enzymatic activity in extraordinary environments
Youhei Yamagata
1
, Hiroshi ... near the C-terminal region on the opposite side of
the N-terminal region on the molecular surface. In the wild-
type enzyme, the side chain of Asp266 interacted with t...
... glycine transfer buffer and
assembled into the transfer membrane sandwich against the
nitrocellulose membrane. All SDS/PAGE bands in the gel
were then transferred onto nitrocellulose membrane ... sequences
and full amino acid sequences of the porcine complex II
subunits. An abundance of gene sequences of mammalian
complex II in the GenBank database was helpful for the...
... 8A).
Next, the levels of Beclin 1 and LC3, two important
proteins involved in autophagy, were examined by wes-
tern blot analysis in L929 cells treated with oridonin.
As shown in Fig. 8B, the level of ... 3-methy-
ladenine or small interfering RNA against LC3 and Beclin 1 promoted
oridonin-induced cell apoptosis. The effects of p38 and nuclear factor-
kappa B in oridonin...
... pyruvate, and meth-
ylamine are bound to the enzyme in this order, and
N-methyl-l-alanine and then NADP
+
are released from
the enzyme. Therefore, the kinetic mechanism of the
enzyme is the same ... substrate and was distinct
from alanine dehydrogenase [18] and N-methylalanine
dehydrogenase found by Lin & Wagner [17]. Lysine
and ornithine were inert as an amine substrate;...
... run the al-
gorithms described in Hu and Liu (2004a) on the
same data as the baseline.
From Table 2, we can see the precision of base-
line system is much lower than its recall. Examin-
ing the ... but can
be inferred from the text. Qi Su et al. (2008) in-
vestigates the problem of extracting those kinds
of features. There approach utilizes the associa-
tion between...
... for murine and human
applications should provide an important advantage in
translation of experimental findings to a clinical setting.
Fig. 4. FPGN2-KBP enhances AdV-GFP infection of human DC, ... CD40-binding peptide that has no detectable
homology to CD40 ligand (data not shown) and that
enhances adenoviral infection of CD40-positive cells of both
human and murine origin, inc...
... easy
editing and utilization. Because all of this can be
achieved with standard hooks and the IME API
of the Microsoft Windows 95 operating system,
this tool can be combined with any Windows- ... consulting
and the contents of the dictionaries for 'thank
you' is displayed. If users double-click on
another word in a conversion window or an
alternatives window...
... deficiency in metazoa.
Considering that IVD acts in the third step of leucine degradation and the
sku mutant accumulates branched-chain amino acids in haemolymph, this
mutant may be useful in the investigation ... cDNA
sequences using the RACE method in both strains
revealed the presence of a single point mutation in
1337 nucleotides of BmIVD. In the sku mutant,...
... lipids of the
lower leaflet to pack more tightly and extend their acyl
chains to maintain the integrity of the membrane. The
substantial tilt (and resulting disorder) of the top leaf-
let lipids and ... [36,37].
The contraction of the lipid headgroups, and con-
comitant disordering of the acyl chains of the lipids in
closest contact with Ab, results in no subs...
... transporters,
the BBB endothelium has a receptor-mediated trans-
porter system used by proteins, such as insulin, trans-
ferrin and leptin, to cross the BBB [13].
Molecular basis of brain angiogenesis
and ... VEGF
expression and induced angiopoietin-1 (Ang-1), thereby
promoting the expression of tight junction proteins and
strengthening the bonds between brain endothelial cell...