Tài liệu Báo cáo khoa học: Amyloidogenic nature of spider silk ppt

... similarities in the nature of spider silk and amyloidogenic ﬁbril formation. This suggests that spider- silk processing, not the least for the large quantities of material produced in vivo in the spider, ... spinning of spider silk. Nature 410, 541–548.2. Simmons, A., Michal, C. & Jelinski, L. (1996) Molecularorientation and two-component nature of the crystalline fraction of spider dragline silk. ... 50 °C(red); (D) fraction of secondary structure of B zone sample for a helix (solid), b sheet (dashed) and other (dotted).Ó FEBS 2002 Amyloidogenic nature of spider silk (Eur. J. Biochem. 269)...

Tài liệu Báo cáo khoa học: "The Nature of Affixing in Written English" docx

... problem of unscrambling English affixes. The role of affixes is crucial in the study of word-breaking practice. In the automatic determination of the parts of speech (a central feature of automatic ... determination of parts -of- speech assignments E. The determination of the number of phonetic syl- lables corresponding to a written English word In the first case, we have taken a random sample of 100 ... the suppressing action of affixes must be understood in detail. In the determination of English citation forms, complete lists of affixes are necessary. The inflection of English verbs is tied...

Tài liệu Báo cáo khoa học: Melatonin Nature’s most versatile biological signal? pdf

... ﬁnal out-come of the treatments. In a recent study, the effect of melatonin (25 lgÆmL)1 of drinking water, % 500 lg perday) on a 10-week-long treatment of male rats with alow dose of methylprednisolone ... & Szabo C (1998) Melatonin inhibitsexpression of the inducible isoform of nitric oxidesynthase in murine macrophages: role of inhibition of NF kappa B activation. FASEB J 12, 685–693.90 ... Switzerland4 Department of Physiology, Faculty of Medicine, University of Buenos Aires, Argentina5 Institute of Zoology, Anthropology and Developmental Biology, University of Goettingen, GermanyKeywordsAlzheimer‘s...

Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc

... however, was devoid of substrate or inhibitor,and consequently did not reveal the nature of theactive site or the mode of action of glyphosate. A dec-ade later, the crystal structure of EPSPS was ... molecular mode of action of glypho-sate and the molecular basis for glyphosate resistance.Structure and function of EPSPSEPSPS catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate ... 4% of thetotal soluble protein content of the cell [48].Fig. 3. Microbial mechanisms of glyphosatedegradation. (A) Two principal pathways of glyphosate degradation are known.Top: cleavage of...

Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt

... of tau with Fyn-SH3. We alsoshow that tau interacts with the SH2 domain of Fyn, and that this associa-tion, unlike that of Fyn-SH3, is inﬂuenced by Fyn-mediated tyrosine phos-phorylation of ... SH3 domain of Fyn binds to proline-richmotifs within the sequence PXXP in interacting pro-teins. Seven such motifs are present in the proline-richdomain of the longest isoform of tau in the ... this band was notapparent on western blots of lysates of Y394F tau(Fig. 2A). The absence of this larger species could indi-cate that phosphorylation of tau by Fyn is reducedand therefore, in...

Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

... determinedby the nature and position of substituents on the phe-nolic ring of the substrate. Second, the redox potential(E°) of the substrate must be low enough, as the rate of the reaction ... buffer at pH 6.0 (25 °C). Structural formulas of the substrates are presented. Redox potentials (E°)ofT1coppers of the laccases and redox potentials of the substrates at pH 4.5 and pH 6.0 are provided, ... diffusion method. Two microlitres of protein solution at a concentration of 9 mgÆmL)1and 2 lL of crystallization solution were equilibrated against 500 lL of reservoir solution. Initial screens...

Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... reservoir consisted of 500 lL of 0.7 m sodium citrate,10 mm dithiothreitol and 70 mm Hepes at pH 7.4. Dropsconsisted of 0.75 l L of importin-a (70-529) at 18 mgÆmL)1,0.75 lL of CLIC4 NLS peptide ... Luria–Bertani medium containing30 lgÆmL)1 of kanamycin. Flasks of 400 mL of Luria–Bertani medium (30 lgÆmL)1kanamycin) were then inocu-lated with 10 mL of the overnight culture. The cells weregrown ... possibility of trigger events to expose NLSsoffers an interesting criterion for cargo selection bynuclear import receptors. Typically, NLSs are identiﬁ-able as a result of their high content of basic...

Tài liệu Báo cáo khoa học: PCR detection of nearly any dengue virus strain using a highly sensitive primer ‘cocktail’ ppt

... comparison.Effect of geographical variation in dengue viruson the performance of the primer cocktailEach of the four serotypes of dengue virus can be classi-fied into several genotypes, defined as a group of ... Yuriy Fofanov1,3and Richard C. Willson1,71 Department of Biology and Biochemistry, University of Houston, TX, USA2 Department of Chemical and Biomolecular Engineering, University of Houston, ... excess of contami-nating human DNA. Serotyping potentially could beachieved by electrophoretic analysis of hybrid products(Fig. 6A, B; all predicted products of amplification of each of the...

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... (Fig. 3A). Three of the ligands,OD2 of Asp165, NE2 of His79 and NE2 of His169,formed an equatorial plane. The other protein ligand,NE2 of His31, bound to the metal, in the company of a water oxygen, ... conformationaround the active site of ApeSOD is independent of the presence of a metal cofactor.Figure 3D shows the superimposition of the activesite structures of apo, Mn-bound and Fe-bound Ape-SODs. ... Japan3 Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Japan4 Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate...

Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... quantitation of microgram quantities of protein uti-lizing the principle of protein-dye binding. Anal Bio-chem 72, 248–254.48 Laemmli UK (1970) Cleavage of structural proteinsduring the assembly of ... report data on homologousexpression of the nrdF gene of C. ammoniagenes strainATCC 6872. This is the ﬁrst report of the successfulpuriﬁcation of high amounts of the native C. ammoni-agenes R2F ... time-scan (B) of a novel enzyme assay; continuous black line, course of enzyme reaction; arrow, time point of substrate addition; triangles, control(reaction by addition of BSA instead of substrate)....

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