Tài liệu Báo cáo khoa học: The solution structure of reduced dimeric copper zinc superoxide dismutase doc

... solve the solution structure of the reduced dimeric protein (by using a classical NMRapproach) in order to compare the solution structures of the monomeric and dimeric species as well as the solution ... dimeric Cu,Zn SOD (Eur. J. Biochem. 269) 1915 The solution structure of reduced dimeric copper zinc superoxide dismutase The structural effects of dimerizationLucia Banci, Ivano Bertini, Fiorenza ... A˚,respectively, for the backbone of the two subunits. The difference between rmsd of monomeric and dimeric speciesis due to the indetermination of the reciprocal orientation of the two subunits. The average...

Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt

... present for the last four residues at the C-terminus. The stereochemistry of the model hasbeen assessed using the program procheck [23]. Ninetyper cent of the residues fall in the most favouredregions ... mol-ecules. When the R-factor decreased to a value of 0.27at 2.0 A˚resolution, inspection of the electron densitymap in the enzyme active site clearly revealed the pres-ence of one molecule of DHAP ... Asp291(2.2 A˚) and the water molecule w1 (2.1 A˚) (Fig. 3A). The DHAP binding site protrudes from the metalN-terC-terFig. 2. Ribbon representation of the overall structure of hACMSD. The (a ⁄ b)8barrel...

Tài liệu Báo cáo khoa học: The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol pptx

... A˚resolution by the molecular replacement method. The schematic view of the overall structure is shown in Fig. 3A.Theenzymeexistsasadimeroftheabc heterotrimer. Thereis a noncrystallographic twofold ... twofold axis around the center of Fig. 3A. The structure of an abc heterotrimer unit is shownin Fig. 3B. The central region of the a subunit constitutes the (b/a)8barrel, the so-called TIM (triosephosphateisomerase) ... tothose in the diol dehydratase-bound cobalamins (2.9–5.1°)[9,10].Figure 4C indicates the comparison of the position of the a-acetamide side chain of pyrrole ring A of the corrin ring in the glycerol...

Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf

... turns of the DNA double helix yields binding of the two orthree NF-Y complexes on the same side of the DNA.Conservation of spacing is required for optimal pro-moter activity because changing the ... However, as in most other CDE ⁄ CHRpromoters, mutation of the CHR results in a smallerremaining cell cycle-regulation than alteration of the CDE [37]. Interestingly, the core of the human Tome-1promoter ... promoters, mutation of the CHRleads to a more pronounced deregulation than the alteration of the CDE [40].Another gene tested for its cell cycle regulation is the cellular inhibitor of apoptosis protein...

Tài liệu Báo cáo khoa học: The capsid protein of human immunodeﬁciency virus: intersubunit interactions during virus assembly doc

... involved either in the assembly of the immatureHIV-1 capsid, or in the reassembly of CA into amature capsid. The detailed description of the structure and conformational dynamics of these interfaces,together ... helix 4 of the NTD of a neighboring subunit in the hexamer.Additional contacts involve helix 11 of the CTD and the C-terminal end of helix 7 of the NTD of the neigh-boring subunit. The NTD–CTD ... Met185Ala). The X-ray model of the NTD hexamer from MLV, the superimposed model of the NTD hexamer fromHIV-1, the high-resolution X-ray model of the modiﬁedCA hexamer from HIV-1, and the pseudoatomic...

Tài liệu Báo cáo khoa học: The capsid protein of human immunodeﬁciency virus: designing inhibitors of capsid assembly ppt

... at Thr188. The two addi-tional aromatic residues in each monomer, Tyr164 andFig. 1. Structure of the CTD dimer. X-ray structure of CTD showing the dimeric structure of the domain. The monomers ... the X-ray structure of the nonmu-tated-CTD–CAI complex [28] (Fig. 2A, main panel). The aromatic moiety of Phe3, together with Leu6 andIle1, are the residues of CAI with the largest number of ... dissociating the nonmutated dimeric CTD (i.e. NYAD-1 does not disrupt the dimeric structure of the wild-type CTD). The residuesobserved in the binding region are the same as in CAI(see above). Furthermore,...

Tài liệu Báo cáo khoa học: The pro-form of BMP-2 interferes with BMP-2 signalling by competing with BMP-2 for IA receptor binding pptx

... Furthermore, the pro-peptide of GDF-8 impairs interaction of the mature part withits receptors [16]. In the case of BMP-9, the pro-pep-tide appears not to alter signiﬁcantly the activity of the mature ... here suggest that the pro-domain of BMP-2 can alter the signalling properties of the growth factorby modulating the ability of the mature part to interact with the receptors.Structured digital ... biological function of the pro-form of bone morphogenetic protein-2 (BMP-2), a member of the transforminggrowth factor b (TGFb) ⁄ BLP superfamily. The role of the 263 amino acids of the pro-peptide...

Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc

... into the chitinolytic poten-tial of L. lactis, the present results provide only the third example of the role of family 33 CBPs in the deg-radation of recalcitrant polysaccharides. Furthermore, the ... [10].Although the occurrence of family 33 CBPs has beenknown for some time [23], the present results provideonly the third demonstration of the accessory function of these proteins. The effect of LlCBP33A ... drawnfor ChiC1 from S. marcescens. The fact that the longerpart of the substrate extends towards the + side of the catalytic centre is compatible with the notion that the C-terminal substrate-binding...

Tài liệu Báo cáo khoa học: The intracellular region of the Notch ligand Jagged-1 gains partial structure upon binding to synthetic membranes docx

... e.g. the case of the association between the N-terminalregion of the membrane-bound tyrosine kinase Lckwith the cytoplasmic tail of the T-cell coreceptors CD4or CD8 [37].In solution, on the ... dispersion of 0.16 p.p.m. (supplementary Figure S1). The lack of chemical shift dispersion in the HN region as well asin the methyl region (data not shown) is an indicator of the lack of globular structure, ... a-helical contentin the protein in the absence of SDS (Fig. 6, Supple-mentary Table S1). The conformation of J1_tmic in the presence of SDSwas further analyzed by NMR spectroscopy. The 1H-15N...

Tài liệu Báo cáo khoa học: The conformational stability of the Streptomyces coelicolor histidine-phosphocarrier protein Characterization of cold denaturation and urea–protein interactions doc

... (enzymeI of the PTS), the first protein in the cascade of proteinsforming the PTS, to HPr, the histidine-phosphocarrierprotein. HPr is the smallest protein in the protein cascade of the PTS and ... from the fitting of the CD thermal denaturation data to Eqn (1). Fitting of the thermal and urea-denaturation data (using the approach of Pace and Laurents [39]) yielded similar values, within the ... described in the literature [1,37]. The errors are calculated from the propagation of fitting errors. DHm¢ , the enthalpy of the cold denaturation, was obtained from fitting to Eqn (1) of the thermal...

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