... 19,
221±240.
Ó FEBS 2002 Signal peptidases of B. amyloliquefaciens (Eur. J. Biochem. 269) 469
Identi®cation and properties of type I-signal peptidases
of
Bacillus amyloliquefaciens
Hoang Ha Chu, ... The
chemistry and enzymology of the type I signal peptidases. Protein
Sci. 6, 1129±1138.
11. Paetzel, M., Dalbey, R.E. & Strynadka, N.C. (2000) The structure...
... expression of T-bet has been
seen within a wide variety of tissues and cells in the
Ginbuna crucian carp [27] and, in mammals, in the
lung tissue of mouse and spleen and thymus of human
and mouse ... the expression
of these genes within humans and mice, of T-bet
within Ginbuna crucian carp [27] and STAT6 in
mandarin fish [28] have shown similar widespread
expression. I...
... properties of SmSmad1 and SmSmad1B as
described above. However, in the presence of SmSmad4
and the active mutant form of TbRI, both SmSmad2
and SmSmad4 interacted with GCN5, indicating the
formation of ... Drosophila MAD and the vertebrate Smad1,
Fig. 1. Structure of the SmSmad1B gene, cDNA and protein. (A) Schematic representations of the SmSmad1B gene, cDNA and pro...
... (lanes 2 and 4) and
32
P-labeled Htelo (lanes 1 and 2) or
rHtelo (lanes 3 and 4). (B) EMSA was performed with RGG3 (lanes
2 and 4) and
32
P-labeled rHtelo (lanes 1 and 2) or mut rHtelo (lanes
3 and ... presence of various amounts of RGG3.
The CD spectrum of Htelo, a hybrid (3 + 1) form,
showed a strong positive band at 290 nm and a nega-
tive band at around 235 nm, whe...
... of the parental
LOS. The LOS band of revertant O35ElpxX also
showed a change from brown to black coloration
(Fig. 4, lanes 3 and 5).
Composition and MALDI-TOF MS analysis of
lipid A in lpxX and ... and structural analysis of OSs in
LOSs of lpxX and lpxL mutants
The OS portion of each LOS was obtained after mild
acid hydrolysis and analyzed for its glycosyl composi-
tio...
... overexpression approach of
RpCAbr and RpCAtr, we cannot know the functional
effect of changes of some key amino-acids.
Origin and number of nonvertebrate CAs
Although the bootstrap values of the deep branches ... 0.5 lL of BSA (10 mgÆmL
)1
),
1 lL of total RNA (1.24 lgÆlL
)1
), 2.5 lL of dNTP (4 mm
total), 1.5 lL of Random Primer 9 (Ozyme, St-Quentin-
en-Yvelines, France...
... HSPs
(HSP70 and HSP23) were greatly and rapidly upregu-
lated in gills but slightly less and later in mantle. One
inducible and two constitutive isoforms of HSP70 are
synthesized in the gills and mantle ... differential
expression of a number of genes that encode elements
of the transcription and translation machinery, includ-
ing transcription factors, ribosomal proteins...
... found to have a molecular mass of 64 kDa, and to contain
two tandem repeats and a Glu-rich region. The structure of the protein
and that of its DNA are similar to those of starmaker, a protein involved
in ... aggregates of
proteins and polysaccharides, and may play important
roles in formation of the phases and ⁄ or morphologies of
the crystals because they consist...
... suspen-
ded in 25 mL of 40% potassium hydroxide and 25 mL of
methanol and refluxed for 2 h. Products were extracted
with 50 mL of hexane. The hexane layer was washed with
25 mL of saturated sodium ... range of structural diversity produced by a
sequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini-
tial origin of structural diversity of skeletons in...
... number of genes in U6A
and U6A-2 cells, IFN-treated U6A-VV-II cells showed
the down-regulation of a substantially larger subset of
genes.
Identification and characterization of a subset of
ISGF3-independent ... Department of Cell and Molecular Biology, Toronto General Research Institute, University Health Network, and Department of
Immunology, University of Toronto, ON,...