Tài liệu Báo cáo Y học: Identiﬁcation and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf

... 2002 A mammalian 14-kDa phosphohistidine phosphatase (Eur. J. Biochem. 269) 5019Cloning and expression of a human 14-kDa phosphohistidine phosphatase Based on the human sequence data a pair of ... existence of a phosphohistidine phosphatase different from protein phosphatase 1, 2A and 2C. A 1000-fold purification to apparent homogeneitygave a 14-kDa phosphatase with a specific activity of 3lmolÆmin)1Æmg)1at ... lgÆmL)1pepstatin A, 100 lgÆmL)1lysozyme and 5 mMEDTA. After freezing,thawing, and ultrasonic treatment the supernatant wascollected and analysed for phosphohistidine phosphatase activity.The...

Tài liệu Báo cáo Y học: Identiﬁcation and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx

... 142–144 amino-acids long; they are acidic and presumably located in the cytoplasm like UspA.Members of this family share a strikingly similar hydro-pathy proﬁle (data not shown), and UP12 shares ... coli.Escherichia coli cells undergo a transition from a rapidgrowth phase to a stationary phase, which is accompaniedby a variety of physiological changes that affect geneexpression, the structure and ... [15]), was ampliﬁed byPCRusinga5¢ complementary deoxyoligonucleotide(5¢-CGCGGATCCATGTATAAGACAATCATTATGC-3¢)containing a BamHI site (underlined nucleotides) and a 3¢deoxyoligonucleotide harboring...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebraﬁsh, Danio rerio docx

... 3¢-RACEZf3¢stat6-F2 CGGTAGTCAGGAAATCAATGCC 3¢-RACEZf5¢stat6-R1 CCATGTCTGCAGATGGTCGAGG 5¢-RACEZf5¢stat6-R2 GGACTGACATTGCTCCAGAGC 5¢-RACEZf3¢stat6-F3 GCTTCAGTGACTCAGAAATTGG 3¢-RACEZf3¢stat6-F4 GTCCAGAATATTCAGCCTTTCACC ... CTGGATTGAAGCGCCCTCGGTTAATC 3¢-RACEZf5¢tbet-R1 GCTGCCTTTGTTATTTGTAAGCTTCAG 5¢-RACEZf5¢tbet-R2 GGAAACTTCCTGTCTCATCCAGTG 5¢-RACEZffoxp3-F1 GGAACACACAGAGGGGATGATA Initial PCRZffoxp3-R1 CTTCAACACGCACAAAGCAC Initial ... GTCCAGAATATTCAGCCTTTCACC 3¢-RACEZftbet-F1 CTCCCTCAAACAAACCAGAGTC Initial PCRZftbet-R1 CACTGGATGAGACAGGAAGTT Initial PCRZf3¢tbet-F1 CTTCTCCAGGACAGTCCAAAGAGTC 3¢-RACEZf3¢tbet-F2 CTGGATTGAAGCGCCCTCGGTTAATC...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf

... 3-day-old and 7-day-oldcultured schistosomules, 15 day, 21 day, 28 day and 35 day para-sites, adult worm pairs, separated adult female and male worms, and eggs. cDNA from uninfected B. glabrata snails ... DeMarco R, Martins EA,Guimaraes PE, Ojopi EP, Paquola AC, Piazza JP,Nishiyama MY Jr, Kitajima JP, Adamson RE et al.(2003) Transcriptome analysis of the acoelomate humanparasite Schistosoma ... transcriptional co-activator P ⁄ CAF potenti-ates TGF-beta ⁄ Smad signaling. Nucleic Acids Res 28,4291–4298.39 Kahata K, Hayashi M, Asaka M, Hellman U,Kitagawa H, Yanagisawa J, Kato S, Imamura...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... Purification and characterization of a sialic acid specific lectin fromthe hemolymph of the freshwater crabParatelphusa jacquemontiiMaghil Denis, P. D. Mercy Palatty, N. Renuka Bai and S. Jeya SuriyaDepartment ... linkages [5] on cell-surface glycocon-jugates, namely glycoproteins and glycolipids, or in bacter-ial polysaccharides. Sialic acids are a family of sugars,N-acetylneuraminic acid (NeuAc) or ... specificactivity. Analysis of the lectin on SDS/PAGE gave a singleband at apparent molecular mass of 34 kDa.The binding affinity of the lectin in the hemolymph of the freshwater crab, Paratelphusa...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulﬁde reductase from methanogenic archaea pdf

... and AF501 overlap by 3 bp). The region 81–65 bp upstream of t he start c odon of AF499 was identiﬁed asan archaeal promoter element by seque nce analysis. The sequ ence AAAGGTTAATATA shows a ... (lane B2). This behavior istypical of integral membrane proteins. The polypeptide with anapparent molecular mass of 53 kDa appears as a double band inunboiled samples (lanes A1 and B1).Table ... indicates that only a half reaction is catalyzed whenonly H -S-CoM is pr esent and that a reaction intermediate of the catalytic cycle is trapped [6]. Variable-temperaturemagnetic circular dichroism...

Báo cáo Y học: Identiﬁcation and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf

... xylosoxydans ssp.xylosoxydans A- 6 N-acyl-D-Asparateamidohydrolase; A- 6-D50061: Alcaligenesxylosoxydans ssp. xylosoxydans A- 6 N-acyl-D-glutamate amidohydrolase; Alicaligenesfaecalis-DA1: Alcaligenes ... identity and 63.6% similarity),N-acyl-D-glutamate amidohydrolase (44.8% identity and 51.2% similarity) and N-acyl-D-asparate amidohydrolase(48.5% identity and 56.5% similarity) from Alicaligenesxylosoxydans ... Ishikara, T. & Fukagawa, Y. (1980) Deacetylation of PS-5, a new beta-lactam compound III. Enzymological char-acterization of L-amino acid acylase and D-amino acid acylasefrom Pseudomonas...

Báo cáo Y học: Identiﬁcation and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is speciﬁcally modulated in thyroid cells by cAMP pot

... redistribution of both actin microfilaments and cytokeratin intermediatefilaments, and with the appearance of a marked cytokeratin and actin immunoreactivity at the cell junctions [28,42]. Thelatter cytoskeleton ... 10)5Mforskolin,an adenylate cyclase activator. A pattern similar to thethyrotropin stimulation was observed in these experimentalconditions.Activation of the tyrosine kinase/MAP kinase pathwayby EGF ... PRK2 kinase is a potentialeffector target of both Rho and Rac GTPases and regulates actincytoskeletal organization. MolCellBiol.17, 2247–2256.13. Watanabe, G., Saito, Y. , Madaule, P., Ishizaki,...

Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc

... TTRTCNCCCATNACRAARTA Cloning of sipUU1 TTGAAYGCNAARACNATHACNYTNAARAA Cloning of sipUV1 TTGAARAARMGNTTYTGGTTYYTNGC Cloning of sipVV2 GTNTTYATNGTYTAYAARGTNGARGG Cloning of sipVV3 TCNGCRTCNSWNATNACNCCNACNAT ... GAGAATTCAAAAGAAAGCGGGGAAGAA Construction of pOpacWhW12 CGAGATCTTGTGGACATGGTCCCGTTTC Construction of pOpacWhS1 CGGAATTCGCTAATGGGAGGAAATCAC Construction of pOpacShS2 TACAGATCTTTTCGTCTTGCGAATTTC ... of sipWW7 TTGTGTAAAAGTGATGACATCGCC Cloning of sipWW8 GTGATCCCGATTATTCTGTGTGTT Cloning of sipWW9 GGCGATGTCATCACTTTTACACAA Cloning of sipWW10 AACACACAGAATAATCGGGATCAC Cloning of sipWW11 GAGAATTCAAAAGAAAGCGGGGAAGAA...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc

... synthase; AGK, N-acetylglutamate kinase; AGPR,N-acetylglutamate 5-phosphate reductase; AOAT, N-acetylornithinetransaminase; GAT, glutamate N-acetyltransferase; AOD, N-acetyl-ornithine deacetylase; ... second-stepenzymes, N-acetylglutamate synthase and N-acetylglut-amate kinase, respectively, are inhibited by arginine.Glutamate N-acetyltransferase is also weakly inhibitedby arginine [16,17]. As a result, ... study, we characterized wild water-melon glutamate N-acetyltransferase (CLGAT) that catalyses the trans-acetylation reaction between acetylornithine and glutamate to formacetylglutamate and ornithine,...

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