Tài liệu Báo cáo Y học: BIGH3 (TGFBI) Arg124 mutations inﬂuence the amyloid conversion of related peptides in vitro Implications in the BIGH3-linked corneal dystrophies pptx

... BIGH3 (TGFBI) Arg124 mutations in uence the amyloid conversion of related peptides in vitro Implications in the BIGH3- linked corneal dystrophies Clair-Florent Schmitt-Bernard1,2, Alain Chavanieu3, ... these disabling diseases.Keywords: amyloidosis; keratoepithelin; lattice corneal dys-trophy; granular corneal dystrophy; synthetic peptide.Hereditary corneal dystrophies are a cause of blindness.These ... Arg124Ser(R124S) [12] in a phenotypic variant of granular corneal dystrophy type 1 (GCD1), Arg124His (R124H) [1] in granular corneal dystrophy type 2 (GCD2, Avellinodystrophy) a mixed type of amyloid and...

Tài liệu Báo cáo Y học: Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA pdf

... methylation of hemimethylated DNA in vitro [17,18]. However, Dnmt1also shows capabilities for de novo methylation of DNA[15]. Interestingly, the de novo activity of Dnmt1 isstimulated by binding ... methyl groups are always introduced, oneslowly by Dnmt3a or by de novo methylation catalyzed byDnmt1 and the second one very fast. Therefore, this effectcan only double the rate of DNA methylation, ... mixture with the activities obtainedwhen equal amounts of the two enzymes are added one after the other (ﬁrst Dnmt3a then Dnmt1) with interveningethanol precipitation of the DNA. In the ﬁrst experiment...

Tài liệu Báo cáo Y học: A novel, inducible, citral lyase puriﬁed from spores of Penicillium digitatum docx

... depend-ence of the conversion was determined by varying the temperature during the incubation from 0.4 to 45 °C. The pH dependence of the conversion was determined byvarying the pH form 5.66–10.19 (by ... as the standard.RESULTSInduction of citral lyaseInitially, the reproducibility of the results was hindered byvariations in the citral lyase activity of the P. digitatumspores. Remarkably, ... furtherincrease of activity. The induction was also stronglydependent on the citral concentration; while there was noinduction in the absence of citral, the activity of the inducedspores increased...

Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by ﬂuorescence spectroscopy pdf

... highbinding affinity of PEP in the presence of Mn2+[28]. The high affinity of PEP in the presence of Mn2+suggests aspecific interaction between these two ligands in the enzymeactive site. In the ... structure of E. coli PEP carb-oxykinase (1OEN) is covalently linked to the carbonyl carbon of the P-pyridoxyl group (PL) through an imino linkage. The green lineshows the protein backbone, the P-pyridoxyl ... that obtained in the presence of Mn2+(Table 1).An independent estimation of the binding affinity of PEPfor E. coli PEP carboxykinase was obtained from the quenching of the emission of Trp residues...

Tài liệu Báo cáo Y học: The glucose-speciﬁc carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx

... wererecently introduced for the preparation of C-6 aldehydederivatives of Glc [15]. The key step is the use of Collinsreagent for the selective oxidation of the primary trimethyl-silyl ether of the ... thatdephosphorylation and/or catalytic turnover of EIIGlc,rather than binding of Glc, enhanced the reactivity of Cys421. As Cys421 is the only invariant cysteine in homologous transporters and also the ... stereoselective synthesis of enantiomericallypure mono- and di-O-hexadecyl-b-D-glucosylglycerol ethers byepoxidation of an allyl b-D-glucopyranoside asymmetricallyinduced by the glucide moiety. Tetrahedron:...

Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is speciﬁcally induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf

... chains of pectin. This is in agreement with aprevious study in which the synergy of enzymes degrading the pectin side chains was studied [34]. GALA had a positiveeffect on the activity of the ... with both the pectinolytic genes encoding mainchain cleaving enzymes as well as with the arabinanolyticgenes [1]. The absence of expression of galA in the presence of pectic galactan indicates ... wall polysaccharides, in particular pectin. Two types of arabinogalactan side chains are present in pectin. Type Iconsists of a chain of b-1,4 linkedD-galactopyranoselinkages, while type II...

Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf

... of Mn[G77Q] and the very low thermostability of Fe[A141Q](Fig. 5). Each of these mutants has two glutamine residues in their active sites; one from the Ônaturally occurringÕresidue, the other engineered ... residues, causing a shift in the position of Y3 4 and a twist in the ring of the metal-binding H81. Thermostability is not a new phenomenonamongst SODs; those isolated from hyperthermophilicorganisms, ... Fig. 5).Effect of temperature on enzyme activity The thermostability of the enzymatic activity of the SODmutants was investigated at 50 °C (Fig. 5). Mn[wt] isinherently less thermostable than...

Tài liệu Báo cáo Y học: The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168 pdf

... characterization of speciﬁc enzymes thatare involved in the biosynthesis of these structures and maylead to the discovery of potential therapeutic targets. In addition, the correlation of carbohydrate ... conclusively demonstrate the linkage of the capsule polysaccharide to its membrane anchor and arecurrently trying to determine the nature of the membranelinkage.It is currently unknown whether the ... cluster,found in the capsule gene cluster, is homologous togenes recently identiﬁed in the Gram-positive, A. thermo-aerophilus, involved in the biosynthesis of D-glycero-D-manno-heptopyranose in the...

Tài liệu Báo cáo Y học: Soluble silk-like organic matrix in the nacreous layer of the bivalve Pinctada maxima A new insight in the biomineralization ﬁeld pptx

... previously [20]. The serine, threonine and tyrosinecontents of the hydrolysates were corrected for destructionduring the hydrolysis by extrapolation to zero time hydro-lysis. The amino acid ... revealed the predominance of two amino acids: alanine (39.2%) andglycine (30%). Interestingly, the proportions are very similarto those found in WSM for alanine and glycine.Curiously, the presence ... conﬁrms the hypothesis that the silk isindeed present in two states, soluble and insoluble, the latterbeing in some way protected from dissolution in water. The peculiarity of the WSM led to the...

Tài liệu Báo cáo Y học: Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species pptx

... appearance of the ThDP adduct with the substratemoiety during the complex-catalyzed succinyl-CoA hydro-lysis [72] accounts for the inactivation by dihydrolipoamide in the presence of succinyl-CoA ... (b) the thiyl radical of the lipoyl residue should efﬁciently interactwith the E1 catalytic intermediate, as the lipoyl-bearingdomain of E2 is designed for this interaction (reaction 2). In this ... inherent in the nitroxides being localizedto the protein interior. Indeed, EPR spectra of the PBNadducts with the protein cysteine residues [66,67] as well as the spectrum obtained in our model...

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