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Tài liệu Báo cáo Y học: Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species pptx

Tài liệu Báo cáo Y học: Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species pptx

Tài liệu Báo cáo Y học: Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species pptx

... that the thiyl radical of the complex-bounddihydrolipoate induces the inactivation by 1e–oxidation of the 2-oxo acid dehydrogenase catalytic intermediate. A pro-duct of this oxidation, the ... pool.Keywords: dihydrolipoate; 2-oxo acid dehydrogenase com-plex; reactive oxygen species; thiyl radical; thioredoxin. The 2-oxo acid dehydrogenase complexes are key mito-chondrial enzymes functioning ... the many-fold dilution of the preincubated mixture upon assay.RESULTSTrapping of radical species in the course of reactionscatalyzed by the 2-oxo acid dehydrogenase complexes The spin trapping...
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Tài liệu Báo cáo Y học: Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA pdf

Tài liệu Báo cáo Y học: Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA pdf

... methyl groups are always introduced, oneslowly by Dnmt3a or by de novo methylation catalyzed byDnmt1 and the second one very fast. Therefore, this effectcan only double the rate of DNA methylation, ... novo methylation of DNA[15]. Interestingly, the de novo activity of Dnmt1 isstimulated by binding of methylated DNA to an allostericsite located in the N-terminal domain of the enzyme[18,19]. ... mainten-ance of the methylation at certain DNA sequences. A role of Fig. 3. Stimulation of Dnmt1 and Dnmt3a by fully methylated CpG sites. In this experiment the rates of methylation of the 310mer...
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Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt

Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt

... orientation of a metal-bound hydroxide for optimalattack on the guanidinium carbon of agmatine. Thus, the disruption of this hydrogen bond is the likely cause of the greately decreased catalytic efficiency ... weconclude that the lower activity of the mutant may beascribed to the loss of an acceptor hydrogen bond to ametal-bound hydroxide, as a consequence of replacement of a carboxylate oxygen with an ... that the environ-ment of tryptophan residues is essentially conserved in the mutant enzyme. On the other hand, the absence of majordifferences in the CD spectra of the wild-type and D153Nagmatinases...
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Tài liệu Báo cáo Y học: A novel, inducible, citral lyase purified from spores of Penicillium digitatum docx

Tài liệu Báo cáo Y học: A novel, inducible, citral lyase purified from spores of Penicillium digitatum docx

... as the standard.RESULTSInduction of citral lyaseInitially, the reproducibility of the results was hindered byvariations in the citral lyase activity of the P. digitatumspores. Remarkably, ... induction.Stability of citral lyase activity The activity and stability of citral lyase was dramaticallyaffected by the addition of 20% (v/v) glycerol and 1 mMEDTA. When glycerol and EDTA were ... monomeric size of these hydratase/aldolase enzymes. All of these enzymesexhibit more then 75% of their maximum activity at pH 7.6, the optimum pH of citral lyase [27,30–32]. Whereas, manybacterial...
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Tài liệu Báo cáo Y học: Studies on the nonmevalonate pathway of terpene biosynthesis The role of 2C-methyl-D -erythritol 2,4-cyclodiphosphate in plants docx

Tài liệu Báo cáo Y học: Studies on the nonmevalonate pathway of terpene biosynthesis The role of 2C-methyl-D -erythritol 2,4-cyclodiphosphate in plants docx

... 2C-methyl-D-erythritol4-phosphate can be converted into a cyclic diphosphate by the consecutive action of 4-diphosphocytidyl-2C-methyl-D-erythritol synthase, 4-diphosphocytidyl-2C-methyl-D-erythritolkinase ... exchange (2% of the isomerization rate) of the methyl group of IPP and the (Z)-methyl group of DMAPP, and an even slower exchange(0.5% of the isomerization rate) of the olefinic hydrogen of DMAPP corresponding ... exchange of the HA-hydrogen of IPP. Inboth cases, scrambling of the label takes place within the IPPpool and the error is then transcribed into the DMAPP pool by the normal action of the isomerase....
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Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc

Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc

... could be the limiting step in BLXI inactivation. Higher Eavalues of inactivation for the Co2þ–enzyme and the Mn2þ–enzyme reflect the higher thermalenergy that these metal–enzyme complexes ... than for xylose,and the relative effectiveness of the three metals was the same. Occupancy rate of the M2 site necessary for activityon glucose may be higher than that for activity on xylose; ... Asboth the Ea of inactivation and the temperature at which the enzyme starts inactivating at a measurable rate increasefrom the apoenzyme to the Mn2þ-containing enzyme, loss of the metal cofactor...
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Tài liệu Báo cáo Y học: Mutations in the docking site for cytochrome c on the Paracoccus heme aa3 oxidase Electron entry and kinetic phases of the reaction pptx

Tài liệu Báo cáo Y học: Mutations in the docking site for cytochrome c on the Paracoccus heme aa3 oxidase Electron entry and kinetic phases of the reaction pptx

... complex of the respiratory chains of mitochondria and many bacteria[1–4]. It catalyzes the reduction of oxygen to water, coupling the free energy of this reaction to the generation of a protongradient ... hypotheticalsecond site have been made here for the bacterial enzyme bystripping subunits III and IV off the native complex, and byfurther destroying a large part of the acidic lobe(s) of the docking ... W121Qactivity, i.e. its (already low) residual electron transferactivity is not maintained by the neighbouring tyrosine,while the W121F /Y1 22F mutant activity may be viewed asa commitment of the tyrosine...
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Tài liệu Báo cáo khoa học: N-Methyl-L-amino acid dehydrogenase from Pseudomonas putida A novel member of an unusual NAD(P)-dependent oxidoreductase superfamily ppt

Tài liệu Báo cáo khoa học: N-Methyl-L-amino acid dehydrogenase from Pseudomonas putida A novel member of an unusual NAD(P)-dependent oxidoreductase superfamily ppt

... N-Methylphenylalanine is the N-terminal amino acid of pilins of most bacterialpathogens expressing type IV pili [7]. The synthesis of these N-methylated peptides is catalyzed by specificN-methyltransferases ... Accord-ingly, we named the enzyme N-methyl-l-amino acid dehydrogenase to distinguish it from the previouslyreported N-methylalanine dehydrogenase [17]. More-over, this study shows that the enzyme ... NADPH, pyruvate, and meth-ylamine are bound to the enzyme in this order, andN-methyl-l-alanine and then NADP+are released from the enzyme. Therefore, the kinetic mechanism of the enzyme is the...
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Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx

Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx

... wererecently introduced for the preparation of C-6 aldehydederivatives of Glc [15]. The key step is the use of Collinsreagent for the selective oxidation of the primary trimethyl-silyl ether of the ... stereoselective synthesis of enantiomericallypure mono- and di-O-hexadecyl-b-D-glucosylglycerol ethers byepoxidation of an allyl b-D-glucopyranoside asymmetricallyinduced by the glucide moiety. Tetrahedron: ... Removal of the trimethylsilyl groupsafforded the methyl ester 1f. This compound was hydro-lyzed under controlled alkaline conditions to the potassiumsalt of the carboxylic acid 1g. The epoxypropyl...
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Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf

Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf

... used.RESULTS The enzymatic activity and molecular stability of the mutants The catalytic activity and the molecular stability of the mutants were assayed because a change in either of these parameters ... seenfrom their inactivation rates in the presence of equimolaramounts of trypsin (Table 2). The cleavage of the interdomain and autolysis loopsTo follow the autolysis of D-chymotrypsin and the mutants, the ... significant restriction in the cleavage of the autolysis loop.Autolytic inactivation of D-chymotrypsin and the Phe114!Ile and Tyr146!His/Asn147!SerD-chymotrypsin mutants The autolytic inactivation rate...
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