... N-Methylphenylalanine is the N-terminal amino acid of pilins of most bacterialpathogens expressing type IV pili [7]. The synthesis of these N-methylated peptides is catalyzed by specificN-methyltransferases ... Accord-ingly, we named the enzyme N-methyl-l-amino acid dehydrogenase to distinguish it from the previouslyreported N-methylalanine dehydrogenase [17]. More-over, this study shows that the enzyme ... NADPH, pyruvate, and meth-ylamine are bound to the enzyme in this order, andN-methyl-l-alanine and then NADP+are released from the enzyme. Therefore, the kinetic mechanism of the enzyme is the...