Tài liệu Báo cáo khoa học: Fluorescence analysis of the Hansenula polymorpha peroxisomal targeting signal-1 receptor, Pex5p pdf

... Fluorescence analysis of the Hansenula polymorpha peroxisomal targeting signal-1 receptor, Pex5p Raina Boteva1, Anne Koek2, Nina V. Visser2, ... investigate the binding of the dansylated PTS1 peptideL2 to HpPex5p and correlate the ﬂuorescence data withstructural data, a molecular model of the HpPex5p wasbuilt based on the known structure of the ... properties of HpPex5p, the PTS1receptor of the yeast H. polymorpha. Fluorescence emissionspectra revealed a pH dependence of Trp ﬂuorescence of HpPex5p, indicative of pH driven changes in the moleculeconformation....

Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll ﬂuorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... amplitudes and rate constants of the two other phases. The above should be gov-erned by the balance between the rate of reductionand oxidation of the acceptor side of PSII by the avail-able electron ... half-time of the J–I phase were bothdecreased with DCMU, demonstrating that the J–I riseis not directly related to the reduction of the PQ pool.A further analysis of the inﬂuence of PQ reductionon ... when the temperature is raised. The effect of DCMU on the traces was to increase the amplitude of step J with the concurrent decline of stepI, and to retard the rise to Fm. With dPQ, the J...

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... with BMAL1 and the bin ding to the E-box.Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box.However, substitution of His57 for Ala ... increase in the mutantmice, the disruption of DEC1 might be compensated byDEC2. Further investigation is required to clarify the functions of DEC1 and DEC2. The suppressive activities of DEC1 and ... connected to the DEC1 E-boxes(Fig. 2), while deletion of the Orange domain ( DEC1DOr-ange) had no effect on the suppressive activity, indicating the importance of t he bHLH region of DEC1. The HXXXXXXXR...

Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx

... mitochondrial proteins, on the otherhand, are specifically linked to one of the major patho-genetic mechanisms of PD [5]. Worthy of note is the complete disappearance of the VDAC-2 upon dopa-mine ... quanti-fied on the basis of their relative volume (spot volume nor-malized to the sum of the volumes of all the representativespots) and those that consistently and significantly variedamong the different ... associated with the combination of factorsanalyzed in the present study (i.e. dopamine together with a-synuclein). Inparticular, the model suggests a potential involvement of the nuclear factorkappa...

Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf

... found between the effects of thesecompounds on the AAA and esterase activities of human BuChE. The concentrations of tyramine and serotonin thatactivate or inhibit the AAA activity of BuChE (andalso ... the titrant. The active siteconcentration of wild-type BuChE was 0.16 ± 0.01 lm,and that of the D70G mutant was 0.196 ± 0.02 lm.Effects of ligands on steady-state kinetics The effects of the ... (bkcatwith b > 1). The degree of activation in the presence of tyraminewas higher for the hydrolysis of o-NAC than for the hydrolysis of o-NPA. This was determined from the nonactivated and...

Tài liệu Báo cáo khoa học: Thermodynamic analysis of porphyrin binding to Momordica charantia (bitter gourd) lectin pptx

... investigated the interaction of several water-soluble porphyrins with MCL. The thermodynamic forces governing the interaction of some of the porphyrins have been delineated from an analysis of the temperature ... investigate the effect of porphyrin b inding on the protein conformation. For thesespectra, a spectrum of the buffer containing the sameconcentration of porphyrin was subtracted from the experimental ... excess of CuTCPP and C uTMPyP are also shown. A ﬁ t of the CDspectrum of native MCL, obtained by analysing the spectrum using the CDSSTRprogram, is also given (details of the spectral analysis...

Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf

... ÔStains-AllÕ analysis of Fig. 3 and the 1D i mmunoblotting of Fig. 4.As the full-length Dp427 isoform of dystrophin does notenter the second dimension of conventional 2D gels, the expression level of ... Incontrast, the exp ression o f major i on-regulatory musclecomponents, such as the Na+/K+ATPase, the SERCA1isoform of the sarcoplasmic reticulum Ca2+ATPase, and the a1-subunit of the dihydropyridine ... over the 2 D gel in a stainless steel tray. The tray was then placed on top of a hot plate and the temperature maintained at 9 0 °C for 5 min to aid the staining of protein spots. The tray was then...

Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

... bond, linkage of the activesite Ser of the protease to the carboxyl group of P1by anester bond and insertion of the N-terminal part of the RCLas strand 4 in b-sheet A (s4A) of the serpin. ... cognateproteases is fuelled by the thermodynamic properties of the serpin. Accordingly, insertion of the RCL as s4A and the ensuing structural rearrangements of the serpin stabilizes the molecule in a so-called ... activity of PAI-1 at the various time-points, i.e. the fraction of the total amount of PAI-1 forminga stable complex with uPA, was calculated from the amount of PAI-1 required to inhibit half the...

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... [26]. These ﬁndingslead to the hypothesis that Fe-bound ApeSOD mimics the product-inhibited form and the shift of Tyr39 sup-presses the release of the peroxide product. This maybe one of the ... inhibi-tion of the binding of the superoxide substrate and the other to product inhibition by hydrogen peroxide.There are two mechanisms proposed for the reactioncycle of Mn ⁄ Fe-SOD: the 5-6-5 ... ligand,NE2 of His31, bound to the metal, in the company of a water oxygen, from the apical positions. The manga-nese was only 0.06 A˚out of the equatorial plane(Table 3). The angles around the metal...

Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... man-ganese in the transcriptional regulation of the nrdoperon [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo ... to the catalytic site of the R1E subunit. Asreported in the Results, upon completion of substrateconversion, the radical is then rapidly passed backfrom the R1E subunit to the tyrosyl of the ... RNR and that of other class 1RNRs or, instead, is a result of the interaction of the R2F with the inactivated (oxidized) form of the R1E.We consider the ﬁrst option unlikely. Under these cir-cumstances,...

Xem thêm