... step.Isolation of PsCIPK and PsCBL cDNA clones and their sequence analysisBasically, both CIPK and CBL cDNAs were first partiallycloned by PCR and then followed by isolation of full-lengthclones by library ... thecytosol.ResultsIsolation and sequence analysis of PsCIPK and CBL cDNAs and genomic clonesFor cDNA cloning, first partial fragments of 550 bp forPsCIPK and 335 bp for PsCBL were amplified by PCRusing double-stranded ... 7A, D, I and L are diamidino-2phenylindolehydrochloride (DAPI) stained cells showing the nuc-leus, and Fig. 7C, F, K and N are the merged images of A and B, D and E, I and J, and L and M, respect-ively.DiscussionNature...