Tài liệu Báo cáo khoa học: Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers ppt
... RNA -binding motif in the
fragile X mental retardation protein (FMRP) and the
G-quadruplex DNA -binding motif in nucleolin [21–
27]. The RGG domain of FMRP, which is an RNA-
binding protein involved ... that RGG3 of EWS binds mainly to the
G-quadruplex.
To test whether formation of the G-quadruplex is
necessary for RGG3 binding, we assayed the binding
of RGG...
... the
inducible chain of the IL-12 receptor (IL-12Rb2).
Binding of IL-12 to its receptor induces signalling
through STAT4, which further enhances IFN-c pro-
duction and induces the expression of ... proteins, a leucine zipper domain
and a C2H2 zinc finger domain, both of which are
thought to help mediate DNA binding and may be
involved in the induction of dimerization...
... bacteria. The difference between
these two mutants in bacterial clearance might reflect
differences in the integrity of their outer membrane,
binding activity and sensitivity of the murine comple-
ment-mediated ... USA) to obtain pCRlpxX. The insert was
released by XhoI–BamHI digestion, and then subcloned
into an XhoI–BamHI site of pBluescript SK(+) to form
pSlpxX. To...
... the binding of ligand to a type II receptor, a
transmembranous serine ⁄ threonine receptor kinase.
The ligand–type II receptor complex recruits another
serine ⁄ threonine transmembrane receptor ... mansoni during stages of
mammalian infection [3]. These results, along with
the reported binding of human TGFb to a chimeric
form of SmTBRI [12], followed by the later find...
... 50% of the isoforms are shown in black and grey, respectively. Histidine residues involved in
zinc binding in the catalytic site are indicated by a ‘Z’; important amino acids involved in the ... detection of RpCAtr mRNA in both
the epidermal cytoplasm of the branchial filaments and
in the cytoplasm of the trophosomal bacteriocytes. We
could only detect RpC...
... number of genes encoding structural components
of the cytoskeleton and proteins involved in contractile
functions (including actin, tubulin myosin and profilin)
were differentially expressed in C. ... assisting the folding of
cytoskeletal proteins that are actively synthesized
and ⁄ or renatured under these conditions. The upregu-
lation of all of these chaperones confirms...
... OMM-64
and otolin-1, are expressed in the same cells in the
inner ear sacculus and are secreted into the extracellu-
lar matrices of the inner ear. In the otoliths, they are
both localized in the ring-like ... to the beads. By contrast, incubation with otolith extract
resulted in binding of a complex of the HMW aggregate containing
OMM-64 and otolin-1 to...
... understanding of the biosynthesis of triterpene
saponins is required, including the enzymes catalyzing
the sequence of reactions and the genes encoding these
enzymes.
The biosynthesis of triterpene ... (Jurkat human T
cell line) by affecting mitochondrial function and are
promising anticancer agents [3]. Despite these promis-
ing activities for medicinal use, great diffi...
... DNA binding, and compared the cDNAs of IFN-treated cells over-
expressing intact STAT2 to those of IFN-treated cells overexpressing
mutated STAT2 lacking the DNA binding domain. The IFN-inducible
expression ... in the absence of the STAT2 DNA
binding domain as revealed by real-time PCR
To more quantitatively examine the expression of IFN-
regulated genes in t...
... of serine proteinases secreted
by various cell lines
Trypsin-type serine proteinases present in the condi-
tioned media of the nine cell lines were analyzed by
gelatin zymography (Fig. 3A). To ... of serine proteinases secre-
ted by nine human cell lines. Conditioned
media were prepared from the cultures of
the indicated, nine human cell lines. The
concentrat...