Tài liệu Báo cáo khoa học: The kinetic properties of various R258 mutants of deacetoxycephalosporin C synthase doc

... of DAOCS by the Kunkel method [20,21].Mutation Primer sequenceR258K 5¢-ACTGGAGGTCTTGCTGCTGC-3¢R258H 5¢-ACTGGAGGTGTGGCTGCTGC-3¢R258A 5¢-ACTGGAGGTCGCGCTGCTGC-3¢R258Q [17] 5¢-ACTGGAGGTCTGGCTGCTGC-3¢R258L ... Sciences Research Coun-cil, Medical Research Council, the Wellcome Trust, the EuropeanUnion and the National Science Council, Republic of China arethanked for ﬁnancial assistance.References1. ... uncoupling may be a consequence of steric interactions between the penicillin substrate and the hydrophobic side-chain of the coproduct (the carboxylic acidthat is analogous to succinate in the...

Tài liệu Báo cáo khoa học: The cellulosomes from Clostridium cellulolyticum Identiﬁcation of new components and synergies between complexes ppt

... of the mini-scaffoldin [21]. The most active mini-cellulo-some on microcrystalline cellulose was composed of the processive cellulase Cel48F combined with endo-glucanase Cel9G. Adding the C. ... efﬁcient association of components. The possible occurrence of synergies between cellulo-somal fractions which might account for the efﬁciency of the cellulosomes was investigated.ResultsFractionation ... such as in XynB (GH11-CBM6 -Doc) from C. thermocellum strain F1 ⁄ YS [36].In the C. cellulolyticum enzyme, the GH11 catalyticmodule had no such catalytic or CBM partner, whichsuggests that the...

Tài liệu Báo cáo khoa học: "The Sentimental Factor: Improving Review Classiﬁcation via Human-Provided Information" docx

... dand the logged co-occurence vector N(w,ak). In thisrespect, the vector of logged co-occurrences is usedto produce derived feature.4 Data Analysis4.1 Accuracy of Unsupervised CoefﬁcientsBy ... avoid co-occurrences that are primarily relevant to othersubjects.• Reproducibility On a ﬁxed corpus, counts of word occurrences produce consistent results.Due to the dynamic nature of the ... a largecollection of documents directly. Then all co-occurrences can be counted in a single pass.Although this approach dramatically reduces the amount of data available, it does offer several...

Tài liệu Báo cáo khoa học: The lipid ⁄ protein interface as xenobiotic target site Kinetic analysis of tadpole narcosis ppt

... characterized lipid ⁄ protein interface of the nAChR as a prototype target site of tadpole narcosis, the microscopic inhibition constants of narcotic chemi-cals need to be known. The KI-value of ... 0.092aLogarithms of octanol ⁄ water partition coefﬁcient.bMicroscopic inhibition constant. c Microscopic lipid dissociation binding constant of aclass of more loosely bound activator molecules binding ... to be most crucial to yield infor-mation on the speciﬁcity of binding of particular vs.bulk lipids, the number of their binding sites and the occupancies and the cooperativity of their potentialinteractions...

Tài liệu Báo cáo khoa học: A kinetic model of the branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis thaliana doc

... time constant of the kinetic controls (about 100 s) then the separation of the kinetic andgenetic controls (an implicit assumption in our model)would be justiﬁed. The characteristics of the ... allosteric controls of the branch-point enzymes are nottaken into account in these experimental studies. Also the occurrence of branch-point two-substrate enzymes and the consequence of their kinetic ... operating condition (leafchloroplast).Fig. 5. Comparison of the kinetic eﬃciencies of CGS and TS. CGS andTS velocities as a function of Phser concentration. v/[CGS] (thin line)was calculated...

Tài liệu Báo cáo khoa học: The tandemly repeated domains of a b-propeller phytase act synergistically to increase catalytic efﬁciency doc

... toincrease the catalytic efficiency of PhyH-DII. Further-more, the catalytic efficiency of PhyH was much greaterthan that of PhyH-DI and PhyH-DII combined (5.73fold) or twice the amount of PhyH-DII ... (嘙 C )Relative activity (%)PhyH PhyH-DIIABCDFig. 2. Environmental factors that affect the catalytic activities of PhyH and PhyH-DII. (A) Effect of 0–5.0 mM Ca2+at 37 C. (B) Effect of ... deposited in the Agricul-tural Culture Collection of China under registrationnumber ACCC 05550.Cloning and sequencing of BPP gene phyHphyH (HM003046) was amplified using degeneratePCR and thermal...

Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

... However, the AGC VIII kinases represent a plant-speci c sub-family characterized by a conserved DFD amino acidmotif in subdomain VII of the catalytic domain andby the presence of an amino acid ... addressesCardiff School of Biosciences, BiomedicalSciences Building, Cardiff, UKàSICIT 2000 S.p.A., Chiampo, Italy§Syngenta Seeds, Enkhuizen, the Netherlands–UMR Ecophysiology and Grape FunctionalGenomics, ... interacts with PTI1-1, PTI1-2,PTI1-3 [7] and PTI1-4, future experiments would benecessary to decipher the speciﬁcity of action of eachcascade and what mechanisms restrict or regulatecross-talk...

Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

... yielding the supernatant LS2, containing the cytosol-enriched fraction, and the pellet LP2, which isenriched in synaptic vesicles. The protein concentrationswere measured using the bicinchoninic acid ... location of p25a was in the cytosolicS3 fraction, and only minor amounts of p25a were pres-ent in the other fractions. The partial co-fractionationwas conﬁrmed by confocal laser scanning microscopicaldetection ... and these species are notformed when DA is protonated at the acidic pH of the storage vesicles. Factors that increase the surfaceexpression of DAT holds the potential to increasecytosolic DA...

Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf

... also include control through p53. Cell cycle arrest can be medi-ated by p53 downregulating the transcription of central cell cycle regulators such as cyclin B, Cks1, Cdc2 and Cdc2 5C. CDE ⁄ CHR-dependent ... combinedwith the change of protein occupation on the Cdc2 5C CDE, indicates that CDE ⁄ CHR sites which regulatecyclin A, cyclin B and Cdc2 promoters may lose bind-ing of their regulator proteins ... depends on the CDE ⁄ CHR, the CHR region and the element upstream from it werereplaced in the human cyclin B2 promoter with the well-characterized CDE ⁄ CHR sites from the humanCdc2 5C and cyclin...

Tài liệu Báo cáo khoa học: The ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C-terminal portion of Bid ppt

... apoptosis. The mitochondrial release of cytochrome c and second mitochondria-derivedactivator of caspase (Smac ⁄ DIABLO) allows for the formation of the apoptosome, a complex that enables the activation ... produced(Fig. 1D, lane 2). In the presence of lactacystin, a sig-niﬁcant increase in the amount of tBid–GFP present in the extract, both in control conditions and in the pres-ence of FLAG–Itch, ... space into the cytosol, resulting in the downstream activation of afamily of cytosolic cysteine proteases, caspases, whichare required for many of the morphological changesthat occur during...

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