Tài liệu Báo cáo khoa học: Three-dimensional structures of thermophilic b-1,4-xylanases from Chaetomium thermophilum and Nonomuraea flexuosa Comparison of twelve xylanases in relation to their thermal stability pdf
... effector-
binding unit composed of the ACT1 and ACT2
Fig. 5. Comparison of a single effector-binding unit between TtAKb-Thr and TtAKb-free. (A) Superposition of the effector-binding units of
TtAKb-Thr ... the ACT2 domain; and (c) two ACT domains,
each from a different chain, interact to form an effec-
tor-binding unit. The effector-binding unit of the
ACT domain in the...
... 279
NADP
+
-binding pocket and restricts the binding
depth of cofactor in a similar fashion to that observed
for OaPDH [12]. In TbPDH, the alanine in this motif
is replaced by glycine suggesting less ... the cofactor-binding domain
of LlPDH are strictly conserved in TbPDH, 89 in
OaPDH. There are 17 residues within 4 A
˚
of the
cofactor, of which 15 are identical in the...
... towards dsDNA, ssDNA and plasmid
were analyzed using linearized pBAD ⁄ gIII plasmid, linea-
rized and denatured plasmid and intact plasmid. The
pBAD ⁄ gIII plasmid was linearized using SalI and ... dena-
tured by incubation at 98 °C in a PCR machine for 3 min,
and then kept on ice. Approximately 300 ng of the various
substrates was mixed with 30 ng enzyme in a total volume
of...
... using
crotonyl-CoA as a substrate [15]. The gene encoding
this bifunctional protein was named AUH (‘AU bind-
ing homolog of enoyl-CoA hydratase’). The RNA-
binding activity of the human protein ... exon 7 and an inser-
tion mutation c.613–614insA. This insertion causes a
frameshift that starts at Met205 and leads to the intro-
duction of a stop codon after four amino acids. The
in...
... se-
mantic relation.
2.2 Machine Learning Algorithms
We are also interested in comparing the perform-
ance of machine learning algorithms on the task of
mapping from n-gram frequencies of joining ... learning algo-
rithms consisted of vectors of web hits obtained
using the 14 most frequent joining terms found in
the BNC. The next condition used a vector of web
hits ob...
... Automatic Collection of Related Terms from the Web
Satoshi Sato and Yasuhiro Sasaki
Graduate School of Informatics
Kyoto University
Sakyo, Kyoto, 606-8501
Japan
sato@i.kyoto-u.ac.jp, sasaki@pine.kuee.kyoto-u.ac.jp
Abstract
This ... configuration
Automatic acquisition of technical terms in a cer-
tain domain has been studied as automatic term
recognition (Kageura and Umino, 1996;...
... English, Hornby and
Covey (1973) (e.g., DeMarcken, 1990). In either
case, such lists are expensive to build and to main-
tain in the face of evolving usage. In addition,
they tend not to include ... out
in Section 1.2.
2.1 SF DETECTION
The obvious approach to finding SFs like "V
NP to V" and
"V
to V" is to look for occurrences of
ju...
... domains
in DNA sequences can provide much insight into the understanding of the
organization and biological functions of the chicken genome. A new seg-
mentation algorithm, which is simple and ... and T
n
are the cumulative numbers of
the bases A, C, G and T, respectively, occurring in the
subsequence from the first base to the n-th base in the
DNA sequence inspected. H...
... of combining
judgments of nugget importance from multi-
ple assessors increases the stability and dis-
criminative power of the evaluation while in-
troducing only a small additional burden in
terms ... nuggets” to assess an-
swers to complex questions. As it has become the
de facto standard for evaluating such systems, the
research community stands to benefit from a be...
... 2003
Three-dimensional structures of thermophilic b-1,4 -xylanases
from
Chaetomium thermophilum
and
Nonomuraea flexuosa
Comparison of twelve xylanases in relation to their thermal stability
Nina ... higher protein density and lower void volumes
indicating better packing.
In the comparison of PDB structures, Karshikoff and
Ladenstein [51]...