... 100 lm of (GlcNAc)1–4was analysed at thestart, in the middle and at the end of each series of sam-ples. The resulting average values of the standards (display-ing standard deviations of < ... 4MU-(GlcNAc)2assubstrate; 50 lL of supernatant was mixed with 50 lLof50 mm citrate–phosphate buffer, pH 3.8, containing 50 lm of substrate and 0.1 mgÆmL)1 of bovine serum albumin,giving a final volume of 100 lL. ... Reaction mixtures contained 1 lm of LlChi18A and ⁄ or 3 lm of LlCBP33A, 0.1 mgÆmL)1 of puri-fied bovine serum albumin, 0.1 mgÆmL)1 of b-chitin powderor 0.5 mgÆmL)1 of a-chitin powder, in 50 mm...