... addition of CoAsolution). The Kmvalue for CoA of the OGORenzyme is reported to be 80 lm [30]. Thus, decarboxyl-ation of 2-oxoglutarate can proceed as a result of CoAcontamination of the acetyl-CoA ... A340as a result of NADHoxidation was measured. One unit of enzyme activity wasdefined as the reduction of 1 lmolÆmin)1 of NADH(corresponding to the carboxylation of 1 lmolÆmin)1 of acetyl-CoA).Quantification ... Ishii1andYasuo Igarashi11 Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan2 Department of Chemistry, School of Medicine, Juntendo University,...