... thior-edoxin-like domains, a, b, b¢, and a¢, a linker region xbetween the b¢ and a¢ domains, and a C-terminal acidicextension. The a and a¢ domains contain the CGHCactive site motif, and are sufficient alone ... is presented as mean ± standarddeviation (number of samples). Statistical significance betweeneach pair of samples with and without bacitracin present wasdetermined using Student’s t-test (two-tailed, ... lm and, when present inthe refolding reaction, PDI was used at 7 lm and bacitracinat 1 mm. The folding reaction was stopped by the additionof 1.1 m iodoacetamide (Sigma-Aldrich), and BPTI and...