... HPLC analysis was performed using an HPLC system (Prominence; Shimadzu, Kyoto,Japan) equipped with an ODS-100S column (150 ·4.6 mm; Tosoh, Tokyo, Japan), and acetonitrile ⁄ metha-nol ⁄ isopropanol ... highly similar to the substrate-binding region ofcytochrome P450 BM-3 [4], whose substrates are long-chain fatty acids. Cytochrome P450 BM-3 has beenengineered to improve activities towards substratessuch ... Guengerich FP (2007) Electron transport pathwayfor a Streptomyces cytochrome P450: cytochrome P450105D5-catalyzed fatty acid hydroxylation in Strepto-myces coelicolor A3(2). J Biol Chem 282, 17486–17500.30...