... Antonella Rosi
1
, Claudio Giovannini
2
,
Orazio Sapora
3
, Vincenza Viti
1
and Laura Guidoni
1
1 Dipartimento di Tecnologie e Salute and INFN Gruppo Collegato Sanita
`
, Istituto Superiore di ... phosphatidylcholine synthesis is reduced or
in the presence of phosphatidylcholine degradation
[18,25]. In particular, inhibition of the final step of
phosphatidylcholine synthesis via...
... classification, based only on
similarities in amino acid sequences, groups together
chitinases and proteins devoid of catalytic activity due
to the substitution of a critical amino acid in the cata-
lytic ... transcripts during
development and in adult tissues
To gain insights into possible physiological functions
of Cg-Clp2, determination of its tissue distribution and
t...
... expression in vivo or
in vitro, including characterization of the promoter
region of the gene and the associated transcriptional
factors. Therefore, delineation of the transcriptional
regulation of ... 1a.
Results
Mapping of transcription initiation sites in the
human DNASE1 gene
To identify the transcription initiation sites of
DNASE1 in pancreas, 5¢-RACE based...
... Maria Ida De Michelis
Dipartimento di Biologia ‘L. Gorini’, Universita
`
di Milano, CNR Istituto di Biofisica – Sezione di Milano, Italy
The H
+
-ATPase is the major electrogenic pump in the
plasma ... C-terminus of isoform 1 of the PM H
+
-ATPase
of A. thaliana (AHA1) interacts with the first 88 amino
acids of PPI1 [22], indicating that the PM H
+
-ATPase
binding site of...
... structure is located
in the C-terminal part of the enzyme.
The subtilisin-like peptidases are dependent on cal-
cium to maintain their stability, and PRK contains
two calcium binding sites, one ... genomic
DNA. Nucl Acids Res 21, 2260.
39 Bradford MM (1976) A rapid and sensitive method for
the quantitation of microgram quantities of protein
utilizing the principle of...
... assumed to participate in the
metabolism of nucleotides. In this context, the assertion that
pyridoxine may be the precursor of the pyrimidine moiety of
thiamin in yeast is intriguing [27]. In fungi, ... defect. The
relationship of all of these genes remains to be clarified.
Expression and purification of Sno1p and Snz1p
In light of the similarity in the amin...
... promoters
[16,25]. In TATA-less promoters, assembly of the pre-
initiation complex relies on binding of multiple general
transcription factors, such as SP-1, in proximity to the
transcription initiation site ... heterologous
desensitization of its signaling in response to the potent
anti-aggregatory ⁄ vasodilatory autocoids prostaglandin
(PG) I
2
(prostacyclin) and nitri...
... detail in both the experimental procedures
(K
i
of cyanidin, bilirubin, biliverdin, section B) and in Table 2 (K
d
of the complexes of bilitranslocase with bilirubin, nicotinic acid and cyanidin
3-glucoside, ... was
assayed to track the progress of the antibody-induced
inhibition. Figure 5A shows that increasing bilirubin
concentrations more and more retarded the p...
... substrate -binding
cleft. It is obvious that elucidation of the 3D structure of
PelB would give more insight into structural organiza-
tion of the binding site.
T. maritima contains at least two evident pectinolytic
enzymes. ... oligoGalpA molecules seems to be in
conflict with its cytoplasmic character and may perhaps
be due to conformational changes in the substrate,
the...
... effective inhibition of
binding of ICAM-4 positive red cells by anti -ICAM-4
antibodies, indicate a major role for ICAM-4 in binding of
red cells to the I domains. The efficient inhibition of the
Fig. ... in binding of
ICAM-4 and the other two ICAMs for the I domains.
Many of these mAbs displayed similar inhibitory profiles in
assays investigating...