Tài liệu Báo cáo khoa học: Multidentate pyridinones inhibit the metabolism of nontransferrin-bound iron by hepatocytes and hepatoma cells docx

... FEBS 2003 Multidentate pyridinones inhibit the metabolism of nontransferrin-bound iron by hepatocytes and hepatoma cells Anita C. G. Chua1, Helen A. Ingram1, Kenneth N. Raymond2 and Erica ... growth and proliferation, is uncertain. Hence, itis of interest to investigate the uptake and metabolism of NTBI in normal and cancer cells, and the effect of Fechelators on these processes.In the ... at 37 °C. The cells were then treated with pronase as described inMaterials and methods. Results are the mean and standard deviation of iron internalized by normal and iron- loaded hepatocytes...

Tài liệu Báo cáo khoa học: MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor pdf

... Institute and Hospital and the National Foundation of Cancer Research. All of the tumor types were confirmed by pathologic analysis. The experiments were undertakenwith the understanding and written ... on the expression of IL6R by RT-PCR and western blot, and found an inverse correlation between miR-23a and IL6R expression at both the mRNA and protein levels.To confirm the direct regulation of ... Both MTT and colony forma-tion assays confirmed the active role of miR-23a in the growth promotion of malignant cells. The fundamental function of miRNAs is to regulatetheir targets by direct...

Tài liệu Báo cáo khoa học: Nuclear receptors in the mosquito Aedes aegypti Annotation, hormonal regulation and expression proﬁling ppt

... AaHNF-4C,lacks the greater part of this A ⁄ B domain and the complete DBD; consequently, it cannot bind DNA[17]. The expression proﬁles of these three AaHNF-4isoforms differ over the course of the vitellogenic ... development of the mostanterior region of the brain [99,100]. Tlx, the verte-brate ortholog of Tll, is required for the correct devel-opment of the visual system and neurogenesis[101,102]. The expression ... in the Ap. mellifera genome, and in situ hybridization studies revealed the presence of this transcript in a small number of cells in the devel-oping eye [20]. This NR and other members of the subfamily...

Tài liệu Báo cáo khoa học: Inorganic phosphate regulates the binding of coﬁlin to actin ﬁlaments pdf

... accelerate the turnover of actin filaments by severing them [3–6], thereby increasing the number of the free pointed and barbed ends, or by increasingmonomer dissociation from the pointed end of fila-ments ... accessiblethrough the closing of the ‘main door’ on the top of the protomer or by some other mechanism. Similarlyin G-actin, cofilin decreases the accessibility of the bound nucleotide, and inhibits nucleotide ... digestions of F-actin revealed that after30 s incubation with cofilin at pH 8.0 the rate and the extent of actin cleavage was the same in the presence and absence of 30 mm Pi. On the otherhand, at...

Tài liệu Báo cáo khoa học: Final steps in the catabolism of nicotine Deamination versus demethylation of c-N-methylaminobutyrate doc

... study and RT-PCR analysis of transcripts. (A) Schematic representation of the pAO1 gene and ORFcluster ﬂanked by Tn554 and DTn. The cluster consists of the pmfR gene, encoding the regulator of the ... expression of these genesdepends on the presence of nicotine in the growthmedium and we have determined the enzyme activities of the proteins. Our results demonstrate the presence of two pathways of ... may enter the citric acidcycle completing the catabolism of the pyrrolidine moiety of nicotine.Expression of the genes of these enzymes was dependent on the presence of nicotine in the growth...

Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf

... enveloped by subunit 7. The arrowslabeled (b) and (c) point to the areas of interaction between the transmembrane helix of subunit 8 and helices G and H1 of cytochrome b and between the N-terminus of ... membrane or lack of detection of the truncated protein by the antibodies.In the W303–1B q° strain the amounts of the other twocatalytic subunits, cytochrome c1 and the ISP, were reduced by about 70–80% ... panels A and B and the relative amounts of each of the subunits determined by densitometry scanning of the stained Westernblots of the q° and CKL57 membranes are shown in panels C and D, respectively.Ó...

Tài liệu Báo cáo khoa học: Differentiation stage-dependent preferred uptake of basolateral (systemic) glutamine into Caco-2 cells results in its accumulation in proteins with a role in cell–cell interaction pptx

... FEBS the cells and separated in one dimension by SDS ⁄ PAGE (Fig. 2). MALDI-TOF MS analysis of 36 clearly visible protein bands covering the entiremolecular mass range of the 1D gel led to the ... relative uptake of radiolabelled glutamine in Caco-2 cells; (b) by assessing the effect of glutamine on the proteome of Caco-2 cells using a 2D gel electrophoresis approach; and (c) by examining ... expressedin the basolateral membranes of epithelial cells in the villi of the mouse intestine [37]. A study performed inCaco2-BBE cells also showed a basolateral localization of LAT-2 [38]. As the Caco2-BBE...

Tài liệu Báo cáo khoa học: Oxygen tension regulates the expression of a group of procollagen hydroxylases docx

... fully support these previous findings and on the otherhand extend them by far and set them in a more generalcontext, as both a-subunits of P4h, PDI as the b-subunit of P4h [13] and the collagen ... up-regulation of the mRNAs of the two P4ha-subunits [aI (P4ha1) and aII (P4ha2)] and of two lysyl-hydroxylases (PLOD1 and PLOD2). These effects of hyp-oxia were mimicked by the iron- chelator ... the question arisesconcerning the mechanisms underlying the orchestratedexpression of these enzymes by the oxygen tension. The findings that the effect of hypoxia on the geneexpression of the...

Tài liệu Báo cáo khoa học: Proteasome involvement in the degradation of the Gq family of Ga subunits pptx

... proteininactivation, and receptor sequestration and degrada-tion that together regulate the duration and or the magnitude of the signaling event. In particular, the rapid degradation of signaling proteins by the ... (GRK2), the kinase that regulates the dur-ation of receptor activation, is mediated by the protea-some [7]. Also, it is becoming increasingly clear that the degradation of members of the family of ... effect on the stability of the proteins. Signiﬁcantly, the turnover of these proteins isnot affected by transient activation of their associated receptors. Fraction-ation studies showed that the...

Tài liệu Báo cáo khoa học: Adaptive changes in the expression of nuclear and mitochondrial encoded subunits of cytochrome c oxidase and the catalytic activity during hypoxia pptx

... switch and alters the expression of the twonuclear genome coded isoforms of CytOX V [10,32]. The regulation of genes CytOX5a and CytOX5b, coding for the two isofoms Va and Vb, parallels that of ... membrane fragments (SMP) by the method of Smith [20], wherein the rate of oxidation of ferrocytochrome cwas measured by following the decrease inabsorbency of its a band at 550 nm. The reaction mediumcontained ... shows the effects of hypoxia on the steadystate levels of nuclear genome encoded CytOX IV and VbmRNAs. There was no change in the levels of these mRNAsin macrophages at 6 h of hypoxia, the time...

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