... hypothesis that Cdc45 may be arate-limiting factor for replication [21]. Our analysisrevealed that 4.5 · 104Cdc45 molecules exist perHeLa S3 or T98G cell (Fig. 7). Previous reports showed that ... reports have shown that the amounts ofhuman Mcm2, -3, -5, -7 and Orc2 remain constantduring the cell cycle [6,41], whereas levels of Cdc6 andCdt1 fluctuate [37,42]. Here, we showed that inHeLa S3 ... during G0 fi G1 that controls entry intothe cell cycle. Mol Cell Biol 23, 2351–2361.29 Bermejo R, Vilaboa N & Cales C (2002) Regulation ofCDC6, geminin, and CDT1 in human cells that undergopolyploidization....