Tài liệu Báo cáo khoa học: Allosteric modulation of myristate and Mn(III)heme binding to human serum albumin Optical and NMR spectroscopy characterization pptx

... Myristate and Mn(III)heme binding to HSA–hemeFEBS Journal 272 (2005) 4672–4683 ª 2005 FEBS 4683 Allosteric modulation of myristate and Mn(III)heme binding to human serum albumin Optical and NMR spectroscopy ... albu-min conformation on the binding of warfarin to human serum albumin. The dependence of the binding of war-farin to human serum albumin on the hydrogen, cal-cium, and chloride ion concentrations ... transition, and appear of generalsigniﬁcance for a deeper understanding of the allosteric modulation of ligand binding properties of HSA.AbbreviationsFA, fatty acid; HSA, human serum albumin; ...

Tài liệu Báo cáo khoa học: Identiﬁcation of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

... Affinity of RGG3 for binding to a G-quadru-plex DNA and RNA.Fig. S2. CD spectra of DNAs and RNAs.Fig. S3. Binding affinity of EWS and RBD to Htelo.Fig. S4. In vitro arginine methylation of RGG3 ... transcriptionalactivator. Cancer Res 61, 2690–2695.51 Ishii S, Imamoto F, Yamanashi Y, Toyoshima K &Yamamoto T (1987) Characterization of the promoterregion of the human c-erbB-2 protooncogene.Proc ... Structural features of EWS and DNA -binding specificities of RGG3. (A) Sche-matic representation of the deletion mutantsconstructed to map the Htelo -binding speci-ficity of each one of the EWS. AD...

Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf

... Philip A. Marsden3 and Gary A. Levy11Multi Organ Transplant Program, Toronto General Hospital and The University of Toronto, Canada;2Department of Pathology and Laboratory Medicine, Texas ... The adding of extracts and cold probe are indicated in the top of the panel. Supershift, ss-Sp1 and ss-Sp3, were shown in presence of anti-Sp1 and anti-Sp3 Ig. Cold and mutated competitors are ... previouslyshown to play a pivotal role in the pathogenesis of murine and human fulminant hepatitis and fetal loss syndrome.Constitutive expression of fgl2 transcripts at low levels areseen in cytotoxic...

Tài liệu Báo cáo khoa học: Specific targeting of a DNA-alkylating reagent to mitochondria Synthesis and characterization of [4-((11aS)-7-methoxy-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepin-5-on-8-oxy)butyl]-triphenylphosphonium iodide doc

... electrode was used to measure the uptake of mitoDC-81 intorat liver mitochondria. To calibrate the electrode ﬁve stepwise addi-tions of 1 lMmitoDC-81 were made to a suspension of mitochondriain ... concentration of mitoDC-81 within mitochondria on incubation of cells with highnanomolar to micromolar concentrations of mitoDC-81. This highlocal concentration of mitoDC-81 within mitochondria ... because of the problem of deliveringDNA to mitochondria, the difﬁculty of generating stableinsertion or expression of exogenous DNA within mam-malian mitochondria, and the large number of mitochon-dria...

Tài liệu Báo cáo khoa học: Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression ppt

... (a component of the NuRDcomplex containing HDAC1 and HDAC2) proteins and to binding of HSF1 to MTA1 [78]. The complexis recruited to the promoters of estrogen-responsivegenes and participates ... (SWI ⁄ SNF, ISW1 and RSC) and histone chaperones (Asf1, Spt6 and Spt16), are involved in histone acetylation and eviction[52–55]. Stress-inducible or constitutive binding of HSF to a promoter is ... repression of mouse heat shock factor 1.Mol Cell Biol 18, 906–918.13 Newton EM, Knauf U, Green M & Kingston RE(1996) The regulatory domain of human heat shockfactor 1 is sufﬁcient to sense...

Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf

... ester).Results and DiscussionAction of reversible effectors on AAA activity of BuChEThe investigation of the effects of the ligands(tyramine, serotonin and benzalkonium) on the AAA and esterase ... siteconcentration of wild-type BuChE was 0.16 ± 0.01 lm, and that of the D70G mutant was 0.196 ± 0.02 lm.Effects of ligands on steady-state kineticsThe effects of the ligands (L: tyramine, serotonin and ... Kss). The concen-tration of tyramine in the assays varied from 0.1 to 15 mm,that of serotonin from 0.05 to 15 mm, and that of benzalkonium from 0.05 lm to 0.5 mm.The type of inhibition or activation...

Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf

... hydrogenbondThepH-activityproﬁlesweresuggestiveofacloserelation-ship of the ionization states in both His57 and His210. To explore this possibility,we attempted to titrate the His57 Nd1 and Ne2 protons by means of 1H -NMR (Fig. 4).A sharp proton signal ... interchange rate of the proton between His57 Nd1-Asp113 Oc2 may be too fast to monitor as the split signals,while at low temperature, that of the interchange rate is tooslow to monitor as the single ... inbovine trypsin and API. The catalytic triadresidues of trypsin and API are Ser195–His57–Asp102 and Ser194–His57–Asp113, respect-ively. The substrate -binding subsite residues of trypsin and API are...

Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc

... ones; and (b) progressive optimization forglyphosate activity is accompanied by improved bind-ing to longer ligands (up to ﬁve atoms in the mainchain) and retained binding to shorter ligands ... some of the key ﬁndings that have led to our current under-standing of the molecular mode of action of glypho-sate and the molecular basis for glyphosate resistance.Structure and function of ... attributed to Ala100 and Leu105 in place of the conserved E. coli and plant resi-dues Gly96 and Pro101 (Fig. 2D). The presence of Ala100 in the CP4 enzyme is of no consequence forthe binding of PEP,...

Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt

... inAb-induced excitotoxicity [11]. Furthermore, Fynsensitizes mice to the toxic effects of Ab [15,16], and tau is required for the Fyn-mediated and Ab-mediatedsynaptic deﬁcits and network impairments ... fusion proteins of Fyn-SH2 and Fyn-SH3 to further investigate the mechanisms by whichtau and Fyn interact. We determined that tau binds to both Fyn-SH2 and Fyn-SH3, and only the former of these associations ... not pos-sible to accurately quantify differences in the relativeproportions of tau capable of binding to Fyn-SH2 and Fyn-SH3, as there may be variations in the afﬁnity of Fyn-SH2 and Fyn-SH3 for...

Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

... molecules A and B (and C and D) of the asymmetric unit form a weakdimer (Fig . 6). On the basis of calculations performedFig. 6. (A) Cartoon representation of dimers of TaLcc1 (blue) and rMaL ... Kruus2,Juha Rouvinen1 and Nina Hakulinen11 Department of Chemistry, University of Eastern Finland, Joensuu, Finland2 VTT Technical Research Centre of Finland, Espoo, FinlandKeywordsascomycete; ... round of reﬁnement were R = 26.7% and Rfree= 34.9%. When atwin operator was included, R-value and Rfree-value weredecreased to 19.2% and 25.2%, respectively. Reﬁnement of the model and twin...

Xem thêm