Tài liệu Báo cáo khoa học: Tet repressor mutants with altered effector binding and allostery docx

... SpringHarbor, NY, USA. Altered TetR effector binding and allostery E M. Henßler et al.4496 FEBS Journal 272 (2005) 4487–4496 ª 2005 FEBS Tet repressor mutants with altered effector binding and allostery Eva-Maria ... molecule.Equilibrium binding constantsa,(·107M)1)[4-ddma-atc-Mg]+[atc-Mg]+TetR TetRi2TetRi2-V99NTetRi2-L17A TetR TetRi2TetRi2-V99NTetRi2-L17ATetRM+ [tc-Mg]+?TetRM[tc-Mg]+0.3b132b119600b1.7b< ... proteinsexhibit residual binding to tetO without effector and with atc. The strongest tetO binding is observed forTetRi2-V99N with 4-ddma-atc as corepressor, whileTetRi2-L17A does not show a...

Tài liệu Báo cáo khoa học: An allosteric DNAzyme with dual RNA-cleaving and DNA-cleaving activities doc

... mM Hepes) and 23 °C with 0.3M NaCl and 10 lML-ascorbate. In (B), the reactions were conducted under different pH conditions with 0.3 M NaCl, 10 lML-ascorbate, and 10 lM CuCl2, and were incubated ... 25.8 and 53.7 °C.When evaluating the optimal design for a DNAzyme with respect to DNA-cleaving and RNA-cleavingactivities, we found that a number of nucleotides withinthe catalytic core and ... lML-ascorbate (except for PL–), and 30 mM Hepes at 23 °C. PL+ and PL) represent the presence and absence, respectively, of L-ascorbate inthe reaction. Lanes M I and M II were loaded with 5¢-32P-labeled...

Tài liệu Báo cáo khoa học: "A SPEECH-FIRST MODEL FOR REPAIR DETECTION AND CORRECTION" docx

... the last and first syllables in the reparandum and repair, respectively, and for normalized f0 and energy; results did not substantially differ from those presented here. port Levelt and Cutler's ... (Cooper and Sorenson, 1977; Gee and Grosjean, 1983; Selkirk, 1984), especially the relative inviolability of noun phrases (Wang and Hirschberg, 1992), and the length of prosodic phrases (Gee and ... to wi-i and to wj; (f) abso- lute and normalized f0 of wi; (g) f0 of wi relative to wi-i and to wj; and (h) whether or not wi was ac- cented, deaccented, or deaccented and cliticized....

Tài liệu Báo cáo khoa học: Dmrt1 genes at the crossroads: a widespread and central class of sexual development factors in ﬁsh pdf

... in the germ cells (Gadus morhua [25] and Danio rerio [24]), whereas much broader and lessrestricted expression territories are seen within the tes-tis. With respect to a gonadal function of ... [20] and Oryzias latipes[21]) would infer a role correlated with Sertoli celllineage specification and subsequently during testiculardifferentiation. The specific expression in spermatogo-nia and ... to male gonadogenesis and further differentiation (Table 1).Dmrt1 expression in protogynous and protandrous hermaphroditic speciesIn hermaphrodite fish (protogynous or protandrous),the developmental...

Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by ﬂuorescence resonance energy transfer–ﬂuorescence lifetime imaging microscopy in living cells doc

... in Figs 1A and S1A, transiently expressedGAPDH–citrine (chimeric protein with N-terminalGAPDH and C-terminal citrine) and citrine–GAPDH(chimeric protein with N-terminal citrine and C-terminalGAPDH) ... protein with N-terminal PGK and C-terminal cerulean) and cerulean–PGK (chimeric pro-tein with N-terminal cerulean and C-terminal PGK)were present throughout the cell. These results agree with ... corresponding totetramers, and no tetramers containing chimeric pro-teins and endogenous GAPDH monomers were found,suggesting that the interfaces needed for the formationof tetramers are mutually...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in human malignancies and potential therapy pdf

... of the available data and mechanismsidentiﬁed in leukemic stem and leukemic tumor cells and link this informa-tion with the known functions of mixed lineage leukemia and certain mixedlineage ... AF10 and DOT1L, and thus compete with their binding to the AF4 complex.An as yet unpublished study has demonstrated thatthe reciprocal AF4–MLL fusion protein retains itsH3K4 HMT activity and ... kinase becomes activated and interacts with promoter-arrested RNA polymerase.Activated P-TEFb kinase then phosphorylates DSIF and NELF. Phosphorylation of AF4, AF9 and ENLturn them into substrates...

Tài liệu Báo cáo khoa học: Basis of recognition between the NarJ chaperone and the N-terminus of the NarG subunit from Escherichia coli nitrate reductase pdf

... monitor the binding of the NarG peptides toNarJ. Surprisingly, the binding isotherm was biphasic, with the best ﬁt obtained with a two binding sitemodel, comprising a ﬁrst site with binding stoichiome-try ... site with binding stoichiome-try (n) of 0.3 ± 0.2 and a binding constant (Kd)of3.4 ± 4 · 10)9m and a second with a stoichiometry of0.7 ± 0.1 and a Kdof 3.3 ± 3 · 10)7m (Fig. 5A).Identical ... of NarG inter-acting with both NarH and NarI subunits withinthe NarGHI complex. Second,1H,15N-HSQC ofNarG(1–28) at natural abundance showed minor shiftsupon NarJ binding (Fig. S1). These...

Tài liệu Báo cáo khoa học: The two Caenorhabditis elegans metallothioneins (CeMT-1 and CeMT-2) discriminate between essential zinc and toxic cadmium docx

... Metal binding stoichiometries and afﬁnities were determined by ESI-MS and NMR, respectively. Both isoformshad equal zinc binding ability, but differed in their cadmium binding behaviour, with ... CeMT-1 and CeMT-2 if presented with Zn : Cd ratios as encounteredby C. elegans. Using a Cd : Zn ratio of 33 : 1 [21 nmCd(II) and 0.7 lm Zn(II)] and 0.1 lm of CeMT-1 and CeMT-2 each, and the ... Caenor-habditis elegans and other species. Log K binding constants forzinc and cadmium metalloforms of C. elegans MTs were deter-mined and compared to those of other MTs. The log K forcadmium binding was...

Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): structure–function relationships docx

... constructed three mutants: the T5S and T5A mutants, with the aim to examinethe role of hydroxyl group; and the DT5 mutant [15].Bioassay results showed that the T5A and DT5 mutants have almost ... cysteine-rich and metal -binding proteins [1].In mammals, four subfamily MTs (i.e. MT1, MT2, MT3 and MT4), have been identiﬁed [2]. MT1 and MT2 areubiquitous isoforms found in most organs and play ... humans, and also Cu, Fe and Zn areheavily deposited in the senile plaque and neuroﬁbriltangle. Any disorder or malfunction in the metallo-proteins and metalloenzymes that maintain and regu-late...

Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): reactivity and structure of metal–thiolate clusters* doc

... [5]Reaction with NO Cysteine oxidation and Zn release Zn release is faster [38] Zn release is slower [38]Reaction with ROSCysteine-oxidation and Zn-releaserates relatively similar [38]Reaction with ... Cu2+ions through reduction to Cu(I) and binding to the protein. In this reaction, thiolateligands are oxidized to disulfides concomitant with Zn2+release. The binding of the first four Cu2+iscooperative, ... This is inline with a lower binding affinity to Cd(II) for MT3compared with MT1 ⁄ 2.One of the most striking features is the greater struc-tural dynamics and flexibility of MT3 and in particularof...

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