Tài liệu Báo cáo khoa học: The Alzheimer b-peptide shows temperature-dependent transitions between left-handed 31-helix, b-strand and random coil secondary structures doc
Tài liệu Báo cáo khoa học: The Alzheimer b-peptide shows temperature-dependent transitions between left-handed 31-helix, b-strand and random coil secondary structures doc
... 3949 The Alzheimer b-peptide shows temperature-dependent transitions between left-handed 31-helix, b-strand and random coil secondary structures Jens Danielsson, Ju¨ri Jarvet, Peter Damberg and ... residue. The bond lengths used were 1.33, 1.45 and 1.52 A˚ and the fixed angles between the bonds were: 58°,64° and 75°. The rotations around the bonds were controlled: fora random coil peptide the ... the Ab monomer is in a domi-nating random coil secondary structure in solution atroom temperature and physiological pH [7–9].Keywordsamyloid b-peptide; b-strand; left-handed 31-helix; random...
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Tài liệu Báo cáo khoa học: The cellulosomes from Clostridium cellulolyticum Identification of new components and synergies between complexes ppt
... enhancement was attributed to the physical prox-imity of the enzymes in the mini-cellulosomes and tocellulose targeting of the complexes via the CBM of the mini-scaffoldin [21]. The most active mini-cellulo-some ... con-trol sample and all the fractions, except for F1, whichwas found to be approximately fivefold more activethan the others. The cellulosomes present in fractionsF1 and F6 therefore have the most ... cohesins and dockerins which determine the composition of the cell-ulosomes [23,34]. Despite the enzymatic diversity of the cellulosomes from C. cellulolyticum (there are 62ORFs encoding dockerin-containing...
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Tài liệu Báo cáo khoa học: The resident endoplasmic reticulum protein, BAP31, associates with c-actin and myosin B heavy chain Analysis by capillary liquid chromatography microelectrospray tandem MS ppt
... a-helicalcoiled -coil structures are known to mediate homo- and hetero-dimerization of proteins, and these sequences mayfacilitate the specific interactions between BAP31 and myosin heavy chain. Furthermore, ... in the BAP31 immunopre-cipitate, indicate that the association between BAP31 and c-actin was the result of specific interactions.DiscussionWe describe in this paper the purification and the ... associations withnewly synthesized IgD and cellubrevin as they exit from the ER to the Golgi apparatus [5] and it has been recentlyshown to participate in the quality control of the cysticfibrosis...
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Tài liệu Báo cáo khoa học: The tandemly repeated domains of a b-propeller phytase act synergistically to increase catalytic efficiency doc
... pH(6.0–8.0) and at low temperatures (0–35 °C). We focuson the function and relationship of the two singledomains. Additionally, the possibility that fusion of the PhyH N-terminal domain to other single-domain ... (Fig. S1). There are two pairs ofcysteine residues, Cys142 and Cys193 in PhyH-DII and Cys445 and Cys495 in PhyH-DI. These residuesare at the same positions as Cys157, Cys206, Cys450 and Cys498 ... with other phytases to release 1.2–2.5-fold phosphate. This is the first time the substratespecificities and functions of the two domains havebeen characterized in a tandemly repeated BPP. The same...
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Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx
... indicatedtime points. The mixes were then incubated with [c-32P]-ATP and MBP (10 l g) for 30 min. The top panel shows the kinase assayvisualized by autoradiography and the bottom panel shows the Coo-massie ... and (B) the top panel shows the kinaseassay visualized by autoradiography and the bottom panel shows the Coomassie Brilliant Blue-stained SDS ⁄ PAGE. The in vitro kinaseassays in (A) and (B) ... as a negative control. The top panel shows the kinase assayvisualized by autoradiography and the bottom panel shows the Coo-massie Brillian Blue-stained SDS ⁄ PAGE. The in vitro kinase assayswere...
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Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf
... in the ChIP assays [60]. Similarly, ChIP experiments on the cyclin A, Cdc2 and cyclin B2 genes suggest that E2F4 and E2F6 bind to the promoters in G0 and to someextent also in G1[55]. Furthermore, ... that the exact binding location is not identified in the experi-ments. Therefore, it is not clear whether the CDE ⁄ CHRis involved in binding these factors.Consistent with a function of p107 and ... proteins and is composed ofp130, E2F4 ⁄ 5 and DP1 ⁄ 2 and a module containing the MuvB proteins Lin-37, Lin-52, Lin-54 and chromatin-associated Lin-9 and Lin-53 ⁄ RBBP4 ⁄ RbAp48.Furthermore,...
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Tài liệu Báo cáo khoa học: The ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C-terminal portion of Bid ppt
... Maintaining the balance between cell survival and apoptosis is critical in the maintenance of a healthy organism, and tipping the equilibrium in one or another direction results in eitherKeywordsapoptosis; ... reagents and the recombinant humanTRAIL ⁄ APO 2 ligand were purchased from Invitrogen and Feldan Bio (St-Laurent, QC, Canada), respectively. The caspase 3 substrate (Ac-DEVD-pNA) and the inhibitorsubstrate ... through the mitochondrial pathway. The key event in the mito-chondrial pathway is the release of proapoptotic fac-tors from the mitochondrial intermembrane space into the cytosol, resulting in the...
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Tài liệu Báo cáo khoa học: The plasminogen activator inhibitor 2 transcript is destabilized via a multi-component 3¢ UTR localized adenylate and uridylate-rich instability element in an analogous manner to cytokines and oncogenes pdf
... of the ARE I-AUUUA and ARE III-AUUUAsequences used primers SJS172 and SJS173, and SJS174 and SJS175, respectively. Mutation of the atypical AU-richsequence used primers SJS259 and SJS260, and ... SJS260, and the creationof the ARE II ⁄ ARE IV double mutant used primers SJS261 and SJS262. The mutagenesis of ARE I and III introducedHindIII restriction sites and so the creation of the pTET-GLO3¢ ... diagrammatic representation of the PAI-2 3¢ UTR showing the location and sequence of the AU-rich regions of interest within the ‘extended ARE’, and the sequences of the various ‘extended ARE’mutants...
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Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc
... isolated and analyzed by northern blot using Fluc and Rluc riboprobes. The radioactive signals were quantified by the STORM 820 equipment and IMAGEQUANT software (Molecular Dynamics). The Rluc ... [15]. On the other hand, other studies indicated that ASF ⁄ SF2activates the translation of mRNAs bearing ASF ⁄ SF2binding sites within the coding region in intact cells[16,54]. The capacity ... between the nucleus and the cytoplasm. Genes Dev 12, 55–66.31 Gui JF, Tronche`re H, Chandler SD & Fu XD (1994)Purification and characterization of a kinase specific for the serine- and arginine-rich...
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Tài liệu Báo cáo khoa học: The two Caenorhabditis elegans metallothioneins (CeMT-1 and CeMT-2) discriminate between essential zinc and toxic cadmium docx
... withthiol groups, and the cadmium speciation of the wild-type and the CeMT-2knockout strain was distinctly different to the CeMT-1 and double knock-outs. Taken together, and supported by a simple ... bear the hallmarks of metallothioneins, i.e. they are small and cysteine-rich, and their expression is induced bymetals [24]. More recently, RNA interference (RNAi) and chromosomal deletion of the ... species, and the data compiledfor Zn6-CeMT-2, Cd6-CeMT-2 and Zn7-CeMT-1 inFig. 1 and Table 1 show that this was achieved byexpression in the presence of the desired metal ion.However, the...
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