Tài liệu Báo cáo khoa học: Peroxiredoxin II functions as a signal terminator for H2O2-activated phospholipase D1 doc

... Peroxiredoxin II functions as a signal terminator for H2O2-activated phospholipase D1 Nianzhou Xiao, Guangwei Du and Michael A. FrohmanDepartment of Pharmacology and the Center for Developmental ... and PMA-stimu-lated CHO cells (63 ·). Rabbit antibody toPrxII and an Alexa-647-labeled goat anti-rabbit IgG secondary were used to visualizePrxII. Mouse monoclonal antibody to HAand an Alexa ... supernatantrecovered for use in the in vitro PLD1 and PrxII interactionassays. All steps were performed at 4 °C.In vitro PLD1 and PrxII binding assayPuriﬁed His6-PrxII, His6-MobA, and PLD1...

Tài liệu Báo cáo khoa học: Cytochrome P450 Cyp4x1 is a major P450 protein in mouse brain doc

... RNA for RNAase protection assay. M indicates the 124-basepair marker transcript (M), the full-length probe is indicated by a line, and the protected fragment is indicated by an arrow. The RNAase ... RNA subjectedto protection assay. The ﬁlm exposure was for 5 days. (C) Heart, kidney, lung and spleen RNA from each of three animals was analysed for Cyp4x1 RNA, and a 5-day exposure of the autoradiograph ... sequence, and was conﬁrmed as the mouse Cyp4x1 on the basis of sequence identity.RNAase protection assay showed that the RNA wasexpressed at high level in brain and in aorta, but thatthere...

Tài liệu Báo cáo khoa học: Computational approaches to understand a-conotoxin interactions at neuronal nicotinic receptors doc

... 2329Exploration at the molecular level of modelsof the neuronal nAChRs As neuronal nAChRs are attractive targets for treating manydiseases such as cognitive dysfunction, neurodegenerationand other ... powerfulcomputational tools available today is facilitating drugdesign at the nAChRs.AcknowledgementsWe thank Joel Tyndall, Christina Schroeder and Ivana Saska for theircomments on the manuscript. ... therapeutic application, whilstmaintaining their other remarkable features. We havegenerated homology models of several nAChR subtypes[2 7a] , so that we can start to analyse in detail the nAChRpharmacophore...

Tài liệu Báo cáo khoa học: "An Affect-Enriched Dialogue Act Classification Model for Task-Oriented Dialogue" doc

... dialogue act. 5.3 Classification Results The classification accuracy and kappa for each specialized classifier is displayed in Table 4. Note that kappa statistics adjust for the accuracy that would ... and adjacent subtasks are semantically more similar than subtasks at a greater distance); therefore, a weighted kappa is also meaningful to consider for this annotation. The weighted kappa is ... & Narayanan, 2009). A recently proposed alternative approach involves treating dialogue utterances as documents within a latent semantic analysis framework, and applying feature enhancements...

Tài liệu Báo cáo khoa học: C-Terminal extension of a plant cysteine protease modulates proteolytic activity through a partial inhibitory mechanism doc

... ex114 aa208 aa24 aaBamH34 aaStartStopXho1N-Pro Protease domainProtease domainCT - ex380 aa1 II NP114 aa208 aaBamH134 aaStartStopXho1SS SSGSSSSN-Pro356 aaIIIMSTLFIISILLFLASFSYAMDISTIEYKYDKSSAWRTDEEVKEIYELWLAKHDKVYSGLVEYEKRFEIFKDNLKFIDEHNSENHTYKMGLTPYTDLTNEEFQAIYLGTRSDTIHRLKRTINISERYAYEAGDNLPEQIDWRKKGAVTPVKNQGKCGSCWAFSTVSTVESINQIRTGNLISLSEQQLVDCNKKNHGCKGGAFVYAYQYIIDNGGIDTEANYPYKAVQGPCRAAKKVVRIDGYKGVPHCNENALKKAVASQPSVVAIDASSKQFQHYKSGIFSGPCGTKLNHGVVIVGYWKDYWIVRNSWGRYWGEQGYIRMKRVGGCGLCGIARLPYYPTKAAGDENSKLETPELLQWSEEAFPLAIV A B66 ... respective mature forms. At eachindicated time, the residual enzyme activity was determined usingan azocasein assay at 65 °C, as described in Materials and meth-ods, and values are expressed as percentage ... protease from the latex of Ervatamia coronaria.Biosci Biotechnol Biochem 62, 1947–1955.15 GuhaThakurta P, Biswas S, Chakrabarti C, Sundd M,Jagannadham MV & Dattagupta JK (2004) Structuralbasis...

Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... importin -a nuclear import pathway, provided that CLIC4 can undergo a conforma-tional rearrangement that exposes the NLS in an extended conformation.DatabaseStructural data are available in ... atoms) and rjis the standard deviation of Bfactors. The normalized B factors have a zero mean and unitvariance. All atoms that satisfy Bz‡ 4 are treated as outliersand discarded. After ... importin -a: CLIC4 NLS peptide interaction.NLS residueInteractions a Van der Waals’ contact area (A ˚2)bBuried surfacearea (A ˚2) RSCCcMain chain Side chain Main chain Side chain TotalVal201...

Tài liệu Báo cáo khoa học: Kinetics of dextran-independent a-(1 ﬁ 3)-glucan synthesis by Streptococcus sobrinus glucosyltransferase I pdf

... Methyl a- d-glucopyranoside was used as a glucose analog that is not a substrate of hexokinase in a glucose assay system. The data were analyzed on thebasis of the Michaelis–Menten equation; the maximumactivity ... weights of activated carbon (Wako PureChemical, Osaka, Japan) and Celite (Wako Pure Chemical)(Fig. S4). The column was maintained at 15 °C, and wasthen washed with water until the glucose was completelyeluted. ... by Neisseria polysaccharea amylosucrase [23].Table 2. Kinetic parameters for the numerical simulation. Simula-tion parameters were estimated as described in Doc. S1.Reactionkcat(s)1)KmSuc(mM)KmAcc(mM)k(s)1ÆmM)1)Sucrose...

Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: a coupled oscillator-based mechanism in smooth muscle ppt

... Pharmacology, University of Calgary, Alberta, Canada2 School of Biomedical Sciences, University of Newcastle, Callaghan, NSW, AustraliaLong-range signalingBiological organs display coordinated ... membrane depolarization activating oradvancing the phase of other Ca2+stores. The electricaland chemical transduction pathways are as depicted inFig. 3. The key mechanisms are as follows: (a) ... nonrhythmic. As has been noted,slow waves are generated by stored Ca2+release [14], a mechanism that requires long-range intercellularsynchronization of oscillatory stored Ca2+release [8].The gastric...

Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: a capacitative (AC) electrical coupling model in neuroepithelium pptx

... MINIREVIEWSynchronization of Ca2+oscillations: a capacitative (AC)electrical coupling model in neuroepitheliumMasayuki YamashitaDepartment of Physiology I, Nara Medical University, Kashihara, JapanStructural ... packed in the basallayer (Fig. 1C). The voltage ﬂuctuations of the Ca2+store will induce ACs, which can pass the series capaci-tance of the ONM and the plasma membrane as capa-citative currents ... nucleoplasm.Capacitative electrical coupling of Ca2+release M. Yamashita294 FEBS Journal 277 (2010) 293–299 ª 2009 The Author Journal compilation ª 2009 FEBSCapacitative [alternating current (AC)]...

Tài liệu Báo cáo khoa học: Intrinsic GTPase activity of a bacterial twin-arginine translocation proofreading chaperone induced by domain swapping ppt

... malachite green GTPase assay. The fraction at 33 mL containedno detectable protein and was assayed as an internal control toestablish that incubation with the column buffers alone did not facil-itate ... glycerol and TMAO and thebenzyl viologen-linked TMAO reductase activity ofperiplasmic fractions was assayed (Fig. 7A) . TorDD68Wwas observed to support assembly of the periplasmicTMAO reductase activity ... discontinuous assay based on detecting released phos-phate using malachite green, and a continuous coupledreaction available commercially as a kit. For the discontin-uous assay, 0.1 mm samples of...

Xem thêm