Tài liệu Báo cáo khoa học: Mammalian mitotic centromere-associated kinesin (MCAK) A new molecular target of sulfoquinovosylacylglycerols novel antitumor and immunosuppressive agents pptx

... Mammalian mitotic centromere-associated kinesin (MCAK) A new molecular target of sulfoquinovosylacylglycerols novel antitumor and immunosuppressive agents Satoko Aoki1,2, Keisuke Ohta1, ... membrane was stainedwith anti-His6Ig and alkaline phosphatase. A single band waspresent that corresponded to the molecular mass of MCAK184(49 kDa).S. Aoki et al. A molecular target of SQAGsFEBS ... diacylglycerols of sea urchin. Trans-plantation 74, 261–267.3 Mizushina Y, Watanabe I, Ohta K, Takemura M,Sahara H, Takahashi N, Gasa S, Sugawara F, Matsuk-age A, Yoshida S & Sakaguchi K (1998)...

Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx

... following apoptotic stimuliresults in the release of cytochrome c and subsequentactivation of caspase-9 and caspase-3, which arecystein proteases that cleave vital cellular targets and cause apoptosis. ... (Iressa). MolCancer Ther 2, 345–351.61 Adam L, Vadlamudi R, Mandal M, Chernoff J &Kumar R (2000) Regulation of microﬁlament reorgani-zation and invasiveness of breast cancer cells byp21-activated ... extracellular ligand-binding domain, a transmembrane domain and an intracellular tyrosinekinase domain. The binding of a ligand to the extracel-lular domain of the EGFR induces receptor dimeriza-tion,...

Tài liệu Báo cáo khoa học: Marine toxins and the cytoskeleton: a new view of palytoxin toxicity ppt

... MINIREVIEWMarine toxins and the cytoskeleton: a new view of palytoxin toxicityM. Carmen Louzao, Isabel R. Ares and Eva CagideDepartamento de Farmacologia, Facultad de Veterinaria, Universidad de Santiago ... Ishida S, Inoue A, Kan Y &Yasumoto T (1995) Palytoxin analogs from the dino-ﬂagellate Ostreopsis siamensis. J Am Chem Soc 117,5389–5390.5 Taniyama S, Arakawa O, Terada M, Nishio S,Takatani ... Na+⁄ K+-ATPase also actsas a signal transducer [44,45]. In this respect, studiescarried out with ouabain, a natural blocker of the Na+pump and an inhibitor of palytoxin action, have pro-vided...

Tài liệu Báo cáo khoa học: Mammalian Gup1, a homolog of Saccharomyces cerevisiae glycerol uptake/transporter 1, acts as a negative regulator for N-terminal palmitoylation of Sonic hedgehog doc

... Taq DNA polymerase(Promega, Madison, WT, USA) using the primers5¢-CACACTACACTGGGAAGCAGAG ACTCCAGC-3¢ and 5¢-AGCTGGCCCAGCAGCCATACACAGTTAAAG-3¢. The cDNA was subcloned into the EcoRV site of ... Tomohiro Chiba for preparation of embryonicday 9.5 mouse embryos; Dr Dovie Wylie and Ms Tak-ako Hiraki for expert assistance; and all members of theDepartments of Pharmacology and Anatomy at KeioUniversity ... indicate that bothpalmitoylated and nonpalmitoylated mammalian Hh proteins can act as signaling molecules. It is nota-ble that while cholesterylation of Hh protein is anintramolecular event catalyzed...

Tài liệu Báo cáo khoa học: Dmrt1 genes at the crossroads: a widespread and central class of sexual development factors in ﬁsh pdf

... of the Y chromosome of themedaka, Oryzias latipes. Proc Natl Acad Sci USA 99,11778–11783.9 Matsuda M, Nagahama Y, Shinomiya A, Sato T,Matsuda C, Kobayashi T, Morrey CE, Shibata N,Asakawa ... Suzuki A, Kobayashi T, Paul-Prasanth B, Lau EL, HamaguchiS, Sakaizumi M & Nagahama Y (2007) DMY geneinduces male development in genetically female (XX)medaka ﬁsh. Proc Natl Acad Sci USA 104, ... Kajiura-Kobayashi H,Suzuki A, Saito N, Nakamoto M, Shibata N &Nagahama Y (2004) Two DM domain genes, DMY and DMRT1, involved in testicular differentiation and development in the medaka, Oryzias latipes....

Tài liệu Báo cáo khoa học: Diol dehydratase-reactivating factor is a reactivase – evidence for multiple turnovers and subunit swapping with diol dehydratase pdf

... K, Hieda N, Yamanishi M, Shibata N &Toraya T (2005) Crystallization and preliminary X-rayanalysis of molecular chaperone-like diol dehydratase-reactivating factor in ADP-bound and nucleotide-freeforms. ... or apoenzyme was used(data not shown). If the a, b and c subunits of theenzyme are abbreviated as a D, bD, and cD, respectively, and the a and b subunits of the reactivase are abbrevi-ated ... cavity is comparable with that of adenine-lacking cobalamins, and thus allows the damagedcofactor to pass through it. Intact cofactor, an ade-nine-containing cobalamin, is not released from theenzyme...

Tài liệu Báo cáo khoa học: Hepatic stimulator substance mitigates hepatic cell injury through suppression of the mitochondrial permeability transition pdf

... IV) antibody to normalize for loading.Statistical analysisAll values are expressed as mean ± standard deviation.Statistical signiﬁcance was determined using a one-wayHSS and mitochondrial ... substance. Am J Physiol242, G289–G295.4 Tanigawa K, Sakaida I, Masuhara M, Hagiya M &Okita K (2000) Augmenter of liver regeneration (ALR)may promote liver regeneration by reducing naturalkiller ... apoptosis,the activation of caspase-3 was examined using theenzymatic Caspase-Glo 3 ⁄ 7 assay. After CCCP treat-ment, caspase-3 activity increased markedly in vector-transfected cells, and this increase...

Tài liệu Báo cáo khoa học: Pronounced adipogenesis and increased insulin sensitivity caused by overproduction of prostaglandin D2 in vivo pptx

... 5¢-GGAGATCTCCAGTGATATCGACCA-3¢ and 5¢-ACGGCTTCTACGGATCGAAACT-3¢ for PPARc ,5¢-AAGACAGCTCCTCCTCGAAGGTT-3¢ and 5¢-TGACCAAATCCCCATTTACGC-3¢ for aP2,5¢-ATCCATGGATGGACGGTAACG-3¢ and 5¢-CTGGATCCCAATACTTCGACCA-3¢ ... 5¢-GCGTCGGGTAGATCCAGTT-3¢ and 5¢-CTCAGTGGGGCTTAGCTCTG-3¢ for ACC, and 5¢-AACACCCCAGCCATGTACGTAG-3¢ and 5¢-TGTCAAAGAAAGGGTGTAAAACGC-3¢ for b-actin. Expression levels of the target genes were normalized to that of ... 5¢-CTGGATCCCAATACTTCGACCA-3¢ for LPL, 5¢-TGGGTTGGCTGCTTGTG-3¢ and 5¢-GCGTGGGCAGGATGAAG-3¢for SCD, 5¢-GATGTGGAACCCATAACTGGATTCAC-3¢ and 5¢-GGTCCCAGTCTCATTTAGCCACAGTA-3¢ forCD36, 5¢-GCGTCGGGTAGATCCAGTT-3¢...

Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): reactivity and structure of metal–thiolate clusters* doc

... are characterized by a conserved array of 20 cysteines and the absence of His and aromatic amino acids. MT3 contains 68amino acids with 70% sequence identity to the MT1 and MT2 (MT1 ⁄ 2) isoforms. ... kinetically labile, allowing rapid intra- molecular and intermolecular metal transfer. This is a direct consequence of the relatively high structuraldynamics and ﬂexibility typical of all MTs ... withROSCysteine-oxidation and Zn-releaserates relatively similar [38]Reaction with PtcompoundsReaction with Cys, Pt bound toCysCisplatin and transplatin reactfaster [36]Cisplatin and transplatin reactslower...

Tài liệu Báo cáo khoa học: Simpliﬁed yet highly accurate enzyme kinetics for cases of low substrate concentrations ppt

... eliminate several statevariables). Such an elimination yields a system of non-linear algebraic equations; analytic solutions of suchsystems are typically unattainable. Hence, high values of m and/ or ... sameorder of magnitude. In all of these cases, the accuracy of Michaelis–Menten kinetics is unsatisfactory as a result of small timescale separation. Particular exam-ples where the QSSA fails ... all scaled variables was set to 0.5 (one-half of the steady state, interms of the unscaled variables zi) and all time trajectories approach zero (as the unscaled variables tend to the steady...

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