Tài liệu Báo cáo khoa học: An engineered disulﬁde bridge mimics the effect of calcium to protect neutral protease against local unfolding docx

... FEBS An engineered disulﬁde bridge mimics the effect of calcium to protect neutral protease against local unfolding Peter Du¨rrschmidt*, Johanna Mansfeld and Renate Ulbrich-HofmannDepartment of ... Model of the 3D structure of the neutral protease from Bacillus stearothermo-philus, including the position of the disulﬁde bridge in mutant G8C ⁄ N60C. The modelwas created on the basis of the ... act as an indicator for the occurrence of the intermediate in the unfolding. Calcium ions are the main contributory factor to enzyme stability The binding of calcium ions seems to be the mainsource...

Tài liệu Báo cáo khoa học: Hypothalamic malonyl-CoA and CPT1c in the treatment of obesity pptx

... nutrient sensing in the brain and is an example of an enzyme that can senseand respond to the nutritional environment. The majorchallenge to understanding the role of CPT1c is to determine its ... duringperiods of an abundant food supply to hedge against periods of food shortage. Today, humans have pushedstorage too far, to the point of widespread obesity.Although obesity is preferable to starvation, ... [14,15,52,53]. Many of these stud-ies rely heavily on inhibitors that may affect the newlyidentiﬁed and structurally similar CPT1c. Therefore,some of these studies need to be re-evaluated in light of the...

Tài liệu Báo cáo khoa học: "Liars and Saviors in a Sentiment Annotated Corpus of Comments to Political debates" pdf

... on the 2009 elec-tion of the Portuguese Parliament. These took place between the 2nd and the 12th of September, 2009, and involved the candidates from the largest Portuguese parties. The ... Sentiment Analysis”. In Proc. of the Joint Hu-man Language Technology Conference and Empiri-cal Methods in Natural Language Processing, Canada. 568Proceedings of the 49th Annual Meeting of the ... annotated. The annotation was per-formed at word and phrase level, and the sentiment expressions identified in the corpus were asso-ciated to the source of the private-state, the target involved and...

Tài liệu Báo cáo khoa học: "From HOPE en I''''ESPERANCE On the Role of Computational Neurolinguistics in Cross-Language Studies " pptx

... mathematically better defined than through other methods, because it enables one to develop a broader perspective of the questions within an analysis of the hypothesis in the context of ... can occur and affect the state of the process. The most important aspect of the control is that all of the serial order computations can occur simultaneously and affect any information that ... "lesion" performance of the model. In this way we are able to study the effect of the "lesion" in a well defined linguistic context, and to make behavioral predictions that can be veri-...

Tài liệu Báo cáo khoa học: "Language Resources Factory: case study on the acquisition of Translation Memories" potx

... deepknowledge of the installation and maintenance of the tool, thus allowing all the other users to benefit16 Evaluation The evaluation of the factory is based on itsfeatures and usability ... monitors the status of the WSs deployed and offers multi-ple metadata fields to annotate WSs.3.3 Workflows: TavernaNow that users can find WSs and use them, the next step is to combine them to ... other for-mats (Ide and Bunt, 2010), e.g. there is software to convert GrAF to UIMA and GATE formats (Ideand Suderman, 2009) and it can be used to mergedata represented in a graph.Both TO...

Tài liệu Báo cáo khoa học: An alternative isomerohydrolase in the retinal Muller cells of a cone-dominant species doc

... (ortho-logue of human RPE65) and retains all of the known key residues for the enzymatic activity of RPE65. Similar to the other RPE-specific RPE65,RPE65c was present in both the membrane and cytosolic ... wereused to identify the subcellular localization of RPE65c and to verify the endoplasmic reticulum membrane preparation. The distribution of RPE65c in each fraction was analyzedby densitometry and ... fractionation To analyze the cellular localization of zebrafishRPE65c in the retina, we generated an antibody usinga specific zebrafish RPE65c peptide, and the specificity of the antibody was confirmed...

Tài liệu Báo cáo khoa học: An anthrax lethal factor mutant that is defective at causing pyroptosis retains proapoptotic activity pdf

... Lethal factor (LF) is the proteolyticcomponent of the toxin, and cleaves six members of the mitogen-activatedprotein kinase kinase family after being delivered to the cytosol by the cell-binding ... 2009 The Authors Journal compilation ª 2009 FEBSpro-IL-1b in the cytosol and the appearance of IL-1bin the cell supernatants (Fig. 2C). A lower level of IL-1b was found in the supernatants of ... concen-tration of 0.5%. The culture was grown for another 4–4.5 h,and then centrifuged at 7000 g for 30 min in a Sorvall Evolu-tion RC centrifuge. The supernatant was decanted into500 mL of autoclaved...

Tài liệu Báo cáo khoa học: An autoinhibitory effect of the homothorax domain of Meis2 ppt

... two of them, the L3-A and YIL-A mutants,whereas the others showed similar activity in this assayas the wild type (Fig. 6C). We next tested the effect of these mutants on activation of the Pbx–Meis ... activity of a linkedAD To further delineate the region required for the inhibi-tory effect of the Hth domain, we created a series of GDB fusion proteins (Fig. 4F). Deletion of either the N-terminal ... whether alternativesplicing of Meis2 or other Meis family members mightaffect the autoinhibitory function of the Hth domain.Database searching revealed the presence of twoisoforms of human...

Tài liệu Báo cáo khoa học: An allosteric DNAzyme with dual RNA-cleaving and DNA-cleaving activities doc

... conserved. A12 can bechanged to T12, and A15 can be changed to G15.When 8–17 DNAzyme binds its substrate, a gÆTwobble pair can be formed, and is considered to besigniﬁcant and crucial for the catalytic ... dramati-cally decreased the rate of DNA self-cleavage. Wesystematically varied the length of the RS to determine the effects on regulation of the DNA self-cleavingactivity. Enlarging the RS to 18 bp essentially ... thatcorresponded to the respective 5¢-terminus of the substrate DNA. The letters indicate the 3¢-termini of these radiolabeled marker DNAs. (C,D) Schemes for the substrate cleavage sites of PL DNAzyme and...

Tài liệu Báo cáo khoa học: An ecdysteroid-inducible insulin-like growth factor-like peptide regulates adult development of the silkmoth Bombyx mori docx

... to affinity chromatogra-phy. Aliquots of the input and the eluate of the chromatographywere resolved by Tricine ⁄ SDS ⁄ PAGE and then analyzed by Coo-massie Brilliant Blue staining (left) and ... achieved.Determination of the structure of 8K-BLPMALDI-TOF MS analysis of the purified materialfrom the final HPLC yielded a monoisotopic mass of 7409.2 ([M + H]+) (Fig. 3A). Amino acid sequenceanalysis of the ... representations of the effects of 8K-BLP on BrdU incorporation into male genitaldisks (J), sperm ducts (K) and flight muscles (L). Values are the means and standard errors of the mean (n = 4–6)....

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