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Tài liệu Báo cáo khoa học: Cold survival in freeze-intolerant insects The structure and function of b-helical antifreeze proteins pdf

Tài liệu Báo cáo khoa học: Cold survival in freeze-intolerant insects The structure and function of b-helical antifreeze proteins pdf

Tài liệu Báo cáo khoa học: Cold survival in freeze-intolerant insects The structure and function of b-helical antifreeze proteins pdf

... Graether and B. D. Sykes (Eur. J. Biochem. 271) Ó FEBS 2004REVIEW ARTICLE Cold survival in freeze-intolerant insects The structure and function of b-helical antifreeze proteins Steffen P. Graether ... verlap of the X-ray structure with the 30 °C NMR structure using the main chain of residues Ser12fiThr70 in the structure a lignment. (C) Overlap of X-ray structure o f TmAFP with theNMR structure ... hat the TmAFP mutant proteins remain mostly well folded [53].Role of the TXT motif and water in activityExamination of the crystal structures of the insect AFPsalso revealed the presence of...
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Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx

Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx

... com-posed of an extracellular ligand-binding domain, atransmembrane domain and an intracellular tyrosinekinase domain. The binding of a ligand to the extracel-lular domain of the EGFR induces ... phos-phorylating the retinoblastoma (RB) protein. Activa-tion of cyclin–CDK complexes is counterbalanced byCDK inhibitors, including those of the kinase inhibitor proteins (KIP) family and the INK4 ... dimeriza-tion, activation of the intracellular kinase domain and autophosphorylation of tyrosine residues within the cytoplasmic domain of the receptor. The tyrosine-phosphorylated motifs of the EGFR recruit...
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Tài liệu Báo cáo khoa học: Emerging pathways in genetic Parkinson’s disease: Potential role of ceramide metabolism in Lewy body disease pptx

Tài liệu Báo cáo khoa học: Emerging pathways in genetic Parkinson’s disease: Potential role of ceramide metabolism in Lewy body disease pptx

... [17–19].Pantothenate kinase is an essential regulatory enzyme in co-enzyme A biosynthesis, catalyzing the cytosolicphosphorylation of pantothenate (vitamin B5),N-pantothenoylcysteine and pantetheine ... destinations.Cells from NPC subjects show a decrease in acidsphingomyelinase activity, leading to the accumulation of sphingomyelin [27]. Because one of the pathwaysfor ceramide recycling is the ... todate. In this minireview, we have brought together datasuggesting that some of the genes involved in the genetics of Lewy body disease, have in common the fact that they impinge on ceramide...
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Tài liệu Báo cáo khoa học: Caveolin-1 influences P2X7 receptor expression and localization in mouse lung alveolar epithelial cells docx

Tài liệu Báo cáo khoa học: Caveolin-1 influences P2X7 receptor expression and localization in mouse lung alveolar epithelial cells docx

... essential for their cor-rect folding, trafficking and function [4–8]. The intracellular C-terminal tail of P2X7is consider-ably longer than those of other members of the P2Xfamily, and contains multiple ... Caveolin-1 staining pattern at the cell surface,confirming the known caveolar location of Caveolin-1. In addition, intracellularly distinguishable punctatepatterns of Caveolin-1 staining were ... band for the remainder of the study. We also assumed that, in immunohistochemical studies, plasma membrane stain-ing reflects the presence of the 80 kDa band alone.Isolation of lipid rafts and...
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Tài liệu Báo cáo khoa học:

Tài liệu Báo cáo khoa học: "Collecting a Why-question corpus for development and evaluation of an automatic QA-system" pdf

... documents). The source of Wik-iXML is the English part of the Wikipedia XMLCorpus by Denoyer and Gallinari (2006). In the original data some of the HTML-structures like lists and tables occurred within ... answers each. The total cost of producing the corpus was about $350, consisting of $310 paid in workers rewards and $40 in Mechanical Turk fees,including all the trials conducted during the devel-opment ... where the partialcontent consisted of the title and headers of the arti-cle, along with the first sentences of each paragraph. The instructions to the questions demanded rigidlythat the question...
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Tài liệu Báo cáo khoa học:

Tài liệu Báo cáo khoa học: "Organizing Encyclopedic Knowledge based on the Web and its Application to Question Answering" ppt

... which do not contain an in- put keyword. Since the extraction module (see Sec-tion 2.3) analyzes the usage of the input term in re-trieved pages, pages not containing the term are of nouse for ... FDDI, d) token ring In the autumn of 1999, out of 80 questions, the num-ber of the first and second types were 22 and 18, re-spectively.4.3 Implementing a QA systemFor the first type of question, ... similar to the description.For the second type of question, human examineeswould search their knowledge base for the description of each of four candidate terms. Then they wouldchoose the candidate...
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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx

Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx

... monitored by the reduction of cytochrome c in the presence of NADH at 550 nm [1].Protein determinationProtein content was determined by the Bradford test [20],using bovine serum albumin as standard.PHI ... explain the regulatory effect. In fact, on addition of PHI, the fluorescence of PHR-bound flavin increases. Thisfinding points to a PHI-induced conformational change of PHR, possibly resulting in ... reaching thermal equilibrium. As shown in the inset, the progressive decrease of the molecular ellipticity atk ¼ 200 nm (the absorption region of the peptide bond)reflects the occurrence of a...
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Tài liệu Báo cáo khoa học: A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana doc

Tài liệu Báo cáo khoa học: A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana doc

... metabolism in the cold. Mathemati-cal modelling and simulation of cold- induced metabolic reprogrammingindicated major differences in the rates of interconversion between the pools of hexoses and sucrose, ... assessment of the trajec-tory of interconversion rates as a function of time of cold exposure, (b) comparison of the magnitudes of the various interconversion rates, and (c) lineup of the accessions ... exposure. The activity of vInv in Rsch increased continuouslyduring cold exposure, and became significantly higherthan in C24 after 7 days at 4 °C. As compared withthat of vInv, the activities of nInv...
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Tài liệu Báo cáo khoa học: Hepatic stimulator substance mitigates hepatic cell injury through suppression of the mitochondrial permeability transition pdf

Tài liệu Báo cáo khoa học: Hepatic stimulator substance mitigates hepatic cell injury through suppression of the mitochondrial permeability transition pdf

... cloning the PCR fragments into the His-tag vector, pET-15b. The recombinant proteins were generated in the E. coli strainOrigami (DE3), and the N-terminal His-tagged proteins were purified using ... opening and cell swelling, an event thatis probably reduced in extent by CsA. CsA specificallyinhibits PTP opening by binding to cyclophilin D in the matrix and on the inner surface of the IM ... whetherHSS could function as an MPT inhibitor, thereby alle-viating hepatic injury and promoting the survival of hepatocytes, after transfection of HSS into the cells or the administration of HSS to...
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Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy in living cells doc

Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy in living cells doc

... upon the distance between and relative ori-entation of two fluorophores, being independent of the strength of protein–protein interactions. In the case of very weak interactions as described in ... constitutinghybrid tetramers. (A) Structure of fluorescent chimeric GAPDH proteins and tetrameric structure of GAPDH. The structure of chimeric proteins isschematically shown on the left. The linkerpeptide ... that induction of FRET does not sim-ply depend on the length of linkers. In the linker con-taining seven amino acids, a Pro-Pro sequence that isnot contained in other linkers is incorporated....
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