Tài liệu Báo cáo khoa học: The conformational stability of the Streptomyces coelicolor histidine-phosphocarrier protein Characterization of cold denaturation and urea–protein interactions doc
Tài liệu Báo cáo khoa học: An alternative isomerohydrolase in the retinal Muller cells of a cone-dominant species doc
... that the enzy-matic activity of RPE65c is iron-dependent. Characterization of the kinetic parameters of the enzymatic activity of zebrafish RPE65cTo determine the steady-state kinetics of the ... the area of the 11cROL and 13cROL peaks and expressed as the mean ± SD of three independent measurements.Retinal cell dissociation The zebrafish eyes were enucleated, and the corneas and lenswere ... (ortho-logue of human RPE65) and retains all of the known key residues for the enzymatic activity of RPE65. Similar to the other RPE-specific RPE65,RPE65c was present in both the membrane and cytosolic...
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Tài liệu Báo cáo khoa học: Stefin A displaces the occluding loop of cathepsin B only by as much as required to bind to the active site cleft doc
... stefin A and the different enzymes is the averagedistance between the centre of mass of CA atoms of the stefin molecule and the centre of mass of the CA atoms of the reactive site cysteine and histidine ... A comparison of the structure of the unli-ganded cathepsin B with the structure of the proenzyme, its complexes withchagasin and stefin A shows that the magnitude of the shift of the occlud-ing ... loop is rather flexible and willadapt to structural features of the inhibitors as well asto the packing constraints of the environment. The lar-ger and wider the features of the ligands that...
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Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase–methionine synthase reductase complex docx
... inset). The absorptionspectrum of the purified enzyme was typical of the hydroxycobalamin form of the enzyme (Fig. 2). The recovery of the activity was 10%, and the enzymewas purified 3669-fold. The ... mediated by other mammalian difla-vin reductases and also subdomains of MSR; (b) the extent of reductive remethylation of hMS catalysed by the different redox states of MSR; and (c) the ability of structurally ... cob(II)alamin. Reduction of the Co centrepromotes the dissociation of the lower dimethylbenz-imidazole base of the cofactor. Consistent with this, the crystal structure of the cobalamin-binding...
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Tài liệu Báo cáo khoa học: Activation loop 3 and the 170 loop interact in the active conformation of coagulation factor VIIa pdf
... 158(21) and 296(154) [14–17]. The high specific activity of theseFVIIa variants is presumably linked to a more stableburial of the N-terminus of the protease domain in the activation pocket of the ... included in the pub-lished alanine scanning mutagenesis study of FVIIa[13], and investigated the effects of the mutation on the enzymatic maturation of FVIIa and on the response of FVIIa to ... cleavage of the scissile bond. This would affect FX and peptidehydrolysis similarly, supported by the similar decreasein the rate of cleavage of both types of substrates. The effect of the G372(223)A...
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Tài liệu Báo cáo khoa học: Metabolic gene switching in the murine female heart parallels enhanced mitochondrial respiratory function in response to oxidative stress pdf
... reaction of the luciferin ⁄ luciferase mixture, energydonor (ATP) and oxygen results in the emission of light. The intensity of the bioluminescence was detected using aluminometer, and the mitochondrial ... measured afteraddition of oxidative substrates, and state 3 respirationafter the addition of 300 lm ADP to the electrodechamber.To test the ability of mitochondria to withstand oxidativestress, ... After 20 min of oxygen lack, the chamber wasreoxygenated for 6 min, and the percentage recovery of state 3 respiration was calculated as the ratio of oxygenconsumption before and after oxygen...
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Tài liệu Báo cáo khoa học: Osmosensing and signaling in the regulation of mammalian cell function docx
... activation of the protein kinase B survival pathway [31], p53 activa-tion [32], induction of the serum- and glucocorticoid-inducible kinase Sgk [33], expression of the heat shock protein Hsp70 [34] and ... summarizes recent progress in the understanding of howosmosensing and osmosignaling integrate into the overall context of growthfactor signaling and the execution of apoptotic programs.AbbreviationsEGF, ... involved in the regulation of metabolism, gene expression and the susceptibilityto different kinds of stress [6]. For example, the inhibi-tion of autophagic proteolysis by insulin, glutamine and ethanol...
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Tài liệu Báo cáo khoa học: Activated Rac1, but not the tumorigenic variant Rac1b, is ubiquitinated on Lys 147 through a JNK-regulated process docx
... following the manufacturer’s proce-dure. Each of the 18 lysine mutants of the myc–Rac1L61sequence and the ubiquitin mutants UbR48 and UbR63were obtained by replacing the lysine codons AAA and AAG ... binding and interac-tion with effectors. The positions of the Lys147 residues arealmost identical. Lys147 of Rac1 is yellow, and the equivalentresidue of Rac1b is shown in orange on top. There ... enzyme(E2), and a target-specific ubiquitin protein ligase (E3),acting sequentially to form an isopeptide bond between the ubiquitin C-terminus and the e-amino group of lysines of the target protein...
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Tài liệu Báo cáo khoa học: Branched N-glycans regulate the biological functions of integrins and cadherins doc
... membrane and mediate interactions with the cytoskeleton and with signaling molecules. On the basis of extensive searches of the human and mousegenomic sequences, it is now known that 18 a-subunits and ... and biological function. For example, only N-glycanslocated on either the b-propeller of a5[55] or the I-likedomain of b1or b3[68] contribute to the regulation of integrin function. Therefore, ... modifi-cation of particular sites, which are involved in regula-tion of the conformation of integrin, determine the extent of cell migration. The mutual regulation of GnT-III and E-cadherinTo...
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Tài liệu Báo cáo khoa học: Chemical approaches to mapping the function of post-translational modifications ppt
... inflammation [17], and immune response [11,12]. The study of these events istaxing, as the biosynthesis of glycoproteins is not tem-plate driven. This results in the formation of so-called‘glycoforms’ ... proteinsmodulate protein activity and greatly expand the diver-sity and complexity of their biological function. The ubiquity of PTMs is reflected in their widespread rolesin signaling, protein folding, localization, ... glycoproteins [33], the firstreported modular total synthesis of a biologically rele-vant glycoprotein. The immediate compatibility of NCL and EPL methods has led to their widespreadadoption. Other...
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Tài liệu Báo cáo khoa học: An intermediate step in the evolution of ATPases – a hybrid F0–V0 rotor in a bacterial Na+ F1F0 ATP synthase pdf
... handedness, and two of the rings face in the opposite direction in the membrane to the other two, forming the same patternas in the AFM surface representation of Fig. 2A. Bycomparison with the ... kDa, and that for the c2 ⁄ 3monomer was 8.3 ± 0.1 kDa. Comparison of the spectra of the complete ring and the fragmentsrevealed a stoichiometry for c1: c2 ⁄ 3 of 1 : 9, leadingto a mass of ... part of the ion-binding site. Therefore, the c ring of A. woodii hasonly 10 membrane-buried negative charges that areessential for binding the ion and also for the rotationalmechanism of the...
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