... covalent modifications of the enzyme, the
effect of the inhibitor on the molecular mass value of
HAD was measured; instead of an adduct increase, or
no change, the value had unexpectedly decreased from
22 ... mod-
ifications [42].
Fig. 9. ECD spectral data from the RNase A deamidation samples of Fig. 8. Deamidation at an individual residue of a specific product causes...
... CP-pyk
(5¢-ACGACTAGTGGATCCATNNNNNAGTTTATTCTT
GACANNNNNNNNNNNNNNTGRTATAATNNNNAA
GTAATAAAATATTCGGAGGAATTTTGAAATGAATA
AACGTGTAAAAATCG-3¢)(N¼ A, T, G, C) and pyk-
back (5¢-CTCTACATGCATTTCAACAATAGGGCCTG
TC-3¢) ... (5¢-TGGTACTCGAG
CAATTTCTGAAGGTATCGAAG-3¢) and pyk2 (5¢-GG
AAGGATCCTTGTGTTTTTCTCCTATAATG-3¢) and
downstream to pyk using primer pyk3 (5¢-GGAAGGA
TCCTTTGTCAATTAATGATCTTAAAAC-3¢) and pyk4...
... bp of the respective 5¢ untranslated
regions and the entire 3¢ untranslated regions. The standard
polyadenylation signals (AATAAA) and the poly (A) -tails
of different lengths are present in each ... crustacean and insect hemocyanins. All analyses support
a pancrustacean taxon of the insect and crustacean proteins
that excludes the myriapod hemocyanins. Within the clade...
... deproto-
nated at neutral pH to make a hydrogen bond with
the side chain of Ser278 and act as a weak general
base catalyst (without the help of Asp82), making an
inefficient surrogate of the c-carboxy ... active
sites of these enzymes contain a unique catalytic triad,
Ser-Glu-Asp, in place of the canonical Ser-His-Asp
triad of the classical serine peptidases. In th...
... this
alignment that all PAS- annotated A. thaliana proteins also
contain a PAC motif, and conversely that all PAC-
annotated A. thaliana proteins contain a PAS domain.
Therefore, in the case of A. ... multiple PAS domains, created a list of
958 PAS sequences. The PFAM-alignment of the PAS
domains was used as an initial alignment. All amino acid
residues extend...
... in
A. thaliana PS. Val111 and Gly113, on the other hand,
are both affected by both pantoate and ATP. These
residues have their positional equivalents in Val137 and
Arg139 in A. thaliana PS, as ... mm.
Purification of the C-terminal domain of PS
The cell lysate was passed through a DEAE anion exchange
column. The protein was eluted with a linear gradient of
NaCl (0–500 mm)...
... that is capable of binding several
different families of transcriptional activators [30], and
evidence indicates that the KIX domain has the ability
to simultaneously interact with at least two ... indicated in blue. The location of the extended loop is indicated with an arrow. (B) The CBP–KIX domain : cMyb binary complex. The
CBP–KIX domain is shown in orange and the...
... hoang ba't ddng san d Hoa Ky.
Bay gid ngUdi ta cho rang nguyen nhan cua
ta't ca cac KH
nay
la
sii
dau cd cua cac ngan
hang
va
cdng ty da qud'c gia de bu
vao
cai da
ma't ...
cac nfldc dang phat trien (A. Panagaraya,
2005):
1.
Cac nUdc da phat trien bao ve bien gidi
va
trd cap rat cao.
2.
Trd ca'p va bao ve ciia cac nUdc da phat
trien ra't cd b...
... Higuchi et al. [30]
to obtain cDNAs encoding M25L–elafin and M63L–trap-
pin. For this purpose, forward primers 5¢-CGACTCGA
GAAAAGAGCGCAAGAGCCAGTCAA-3¢ and 5¢-CGAC
TCGAGAAAAGAGCTGTCACGGGAGTTCCT-3¢ ... oxidized elafin and trappin. The data
for the native inhibors are from Zani et al. [15]. The k
diss
and K
i
values are experimental, whereas the k
ass
values are calculated. MPO,
myeloperox...
... measured at the
interface between a solid and a liquid phase, and cal-
culated as the ratio of two rate constants. However,
values of DDG and DDG¢ for mutant Fab fragments,
calculated from values ... wells of a microtiter plate and the bound
Fab4E11-H6 was revealed with a goat antibody, directed against
mouse Fab and conjugated with alkaline phosphatase. The total
concen...