Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

... the presence of 3Mguanidinium chloride.1166 M. Riboét al. (Eur. J. Biochem. 27 1) Ó FEBS 20 04 Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization ... (Met1)-ONC(M23L) by the addition of AAP, as described in the Materials and methods. Completion of the hydrolysis wasconfirmed by the disappearance of the (Met1)-ONC(M23L) signal in the MALDI-TOF mass ... evaluating the influence of Table 2. Thermodynamic parameters of the thermal denaturaturation of the different onconase (ONC) variants at 25 °C and pH 2. 0. (CGdm/HCl)½13,midpoint of guanidinium...

Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll ﬂuorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... raised. The effect of DCMU on the traces was to increase the amplitude of step J with the concurrent decline of stepI, and to retard the rise to Fm. With dPQ, the J stepwas lowered and the subsequent ... depends on the relative amplitude and rate constant of the J–Iphase compared to the amplitudes and rate constants of the two other phases. The above should be gov-erned by the balance between the ... present, which occurs at the I step [33]. The origin of both the J–I and I–P phases, withhalf-times of 7.4 ± 0.6 ms and 42 ± 3 ms, has oftenbeen attributed to the reduction of the PQ pool. Someauthors...

Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx

... FbPbObservedchangeRPLP2 P05387 11.7 4. 42 4 26 8 53 8.41 0. 020 DA fl·3.9Parathymosin P209 62 11.4 4.14 2 88 22 12. 14 0.008 DA fl 2. 3eIF5A isoform B P6 324 1 16.8 5.08 2 70 7 12. 72 0.007 DA fl 2. 1L7 ⁄ L 12, mitochondrial ... mitochondrial P 528 15 21 .4 5.37 3 140 11 9 .28 0.016 DA fl·1.6Peroxiredoxin 1 Q06830 22 .1 8 .27 9 431 45 10.39 0.0 12 DA ›·1.5Annexin A5 Q6FHB3 35.9 4.83 13 720 52 6. 72 0.0 32 DA fl·1.6Annexin A2 Q8TBV2 38.6 ... 6 29 9 20 5 .25 0.050 DA ›·1.6Aldolase A P04075 39.3 8.34 5 21 0 20 36.86 0.001 DA › 2. 5Fascin 1 Q16658 54.5 6.84 7 333 16 5.88 0.0 42 DA › 2. 3Pyruvate kinase P14618 57.8 7.95 12 521 29 7 .22 0. 028 ...

Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf

... [S]cross.Modulation of butyrylcholinesterase catalytic activitiy P. Masson et al. 26 20 FEBS Journal 27 5 (20 08) 26 17 26 31 ª 20 08 The Authors Journal compilation ª 20 08 FEBSboth the competitive inhibition of hydrolysis ... catalytic activitiy P. Masson et al. 26 28 FEBS Journal 27 5 (20 08) 26 17 26 31 ª 20 08 The Authors Journal compilation ª 20 08 FEBSterms of binding of the positively charged ligands toPAS. This would ... in the plasma of nor-mal subjects is about 7 nm [63]; it is increased as aSer198Ser 224 ABSer 224 Ser198His438Glu 325 watwat 2. 7 2. 8 2. 5 2. 9 2. 7Fig. 6. (A) Overall view of the three-dimensional...

Tài liệu Báo cáo khoa học: Thermodynamic analysis of porphyrin binding to Momordica charantia (bitter gourd) lectin pptx

... (kJÆmol)1)CuTMPyP 20 .0 1.01 6.36 ) 27 .40H 2 TMPyP 20 .0 0.99 4.49 ) 26 .55CuTCPP 32. 2 0.97 2. 97 ) 25 .53H 2 TCPP 48 .2 1.03 2. 84 ) 25 . 42 ZnTPPS 65.6 1. 02 1.10 ) 23 .07H 2 TPPS 34 .2 1.05 0.58 ) 21 .48Table 2. ... 90.8 25 (6.80)30 4.35H 2 TMPyP 20 6.60 25 4.49 ) 59.5 ) 110.830 2. 6735 2. 1035 (2. 15)CuTCPP 10 25 . 32 15 10 .26 20 5.85 25 2. 97 ) 98.1 ) 24 3.9 25 (3.70)H 2 TPPS 20 0.98 25 0.58 ) 85.3 ) 21 4.730 ... of MCL and the effect of ligand binding on it, the far-UV CD spectra of MCL in the native state as well asin the presence of CuTCPP and CuTMPyP were analysedby the CDSSTRprogram using the routines...

Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf

... to the Dp 427 isoform of dystroph in (A), the a-subunit of the d ystroglycan complex ( a-DG; B), the N a+/K+ATPase (N KA, C), the fast SERCA1 i soform of the sarcoplasmic r et iculum Ca 2+ ATPase(D), ... t he 2D ÔStains-AllÕ analysis of Fig. 3 and the 1D i mmunoblotting of Fig. 4.As the full-length Dp 427 isoform of dystrophin does notenter the second dimension of conventional 2D gels, the expression ... CSQ), and calmodulin (CAM), respectively. The pH values of the ﬁ rst dimensiongel system and molecular mass standards (in kDa) of the seconddimension are indicated at the top and on the left...

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... genes, including Dec2 and PPARc2,intheabsence of other transcription facto rs, such as CLOCK and BMAL1 [9,10 ,22 ,23 ]: an N-terminal region between aminoacids 1 and 141, and another region between ... ility for the E-box (lane 7), and substitution of Arg65 for Ala(DEC1-R65A and DEC1:4– 23 32- R65A) abolished DEC1 binding to the E-box (lanes 6 and 16). On t he other hand, deletion of up to 27 3 residuesfrom ... Dec2 expression was f ound to increase in the mutantmice, the disruption of DEC1 might be compensated byDEC2. Further investigation is required to clarify the functions of DEC1 and DEC2.The...

Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc

... 1186–1191. 23 02 FEBS Journal 27 2 (20 05) 22 92 23 03 ª 20 05 FEBSControl analysis of the las operon B. Koebmann et al.on the TP901-1 attB locus on the chromosome of MG1363. The replication-thermosensitive ... Koebmann et al. 22 96 FEBS Journal 27 2 (20 05) 22 92 23 03 ª 20 05 FEBSABFig. 6. Modulation of the las operon. (A) Enzyme activities of PFK, PK and LDH relative to the wild-type level. The enzyme activities ... integration of extra gene copiesB. Koebmann et al. Control analysis of the las operonFEBS Journal 27 2 (20 05) 22 92 23 03 ª 20 05 FEBS 22 99References1 Kacser H & Burns JA (1973) The control of ﬂux....

Tài liệu Báo cáo khoa học: Quantitative estimation of channeling from early glycolytic intermediates to CO2 in intact Escherichia coli pdf

... mutant 2. 17 0.0 42 ± 0.001Fru1,6P 2 RK9117 mutant 4.05 ± 0. 72 0.0 92 ± 0.001Quantitation of channeling in intact E. coli G. Shearer et al. 326 2 FEBS Journal 27 2 (20 05) 326 0– 326 9 ª 20 05 FEBS the ... [14C]Fru1,6P 2 to14CO 2 in the presence of [ 12 C]glucose. The data in Figs 2 and 3, considered separately,are insufficient to determine the predicted result ifTable 2. Fraction of the total flux to14CO 2 via the ... 0.155 ± 0.006 (P ¼ 0.017) 0 .22 9 ± 0.145 NSQuantitation of channeling in intact E. coli G. Shearer et al. 326 6 FEBS Journal 27 2 (20 05) 326 0– 326 9 ª 20 05 FEBS the product of the first enzyme to have...

Tài liệu Báo cáo khoa học: "Contextual Analysis" doc

... of the "determining" words which will enable us to fix the semantic values of the "determineеs". The result of the aggregate of structural comparisons of this kind, and ... governs the genitive case. The task of deciding whether or not to retain the &apos ;of& apos; falls upon the reader. The problem results, of course, from the syntactical com- pactness of inflected ... &apos ;the addition the mixtures', as well as the translation given above; 2) the &apos ;of generated by the genitive case is often disregarded, under the condition, for example, that the...

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