Tài liệu Báo cáo khoa học: Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma cruzi epimastigotes docx

... T. cruzi FEBS Journal 273 (2006) 628–637 ª 2006 The Authors Journal compilation ª 2006 FEBS 637 Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma ... to a-amanitin of the transcription of arginine decarboxylase (ADC) and cruzipain genes in transformed Trypano-soma cruzi. Permeable parasites were preincubated at 0 °C for5 min in the absence ... con-taining DNA spots (5 lg each) corresponding to inter-nal fragments of ADC or cruzipain genes. The latter(used as a control) is a housekeeping gene that encodesthe main cysteine proteinase of...

Tài liệu Báo cáo khoa học: Modulation of a-synuclein aggregation by dopamine in the presence of MPTP and its metabolite pptx

... line), in the presence of 100 lM neurotoxin (•, dotted line), in thepresence of 200 lM neurotoxin ( , dottedline) and in the presence of neurotoxin and 50 lM dopamine (h, dashed line). Modulation ... occur in the brain includeselective loss of dopaminergic neurons in substantianigra pars compacta and appearance of Lewy bodiesconsisting of aggregated protein, mainly a-synuclein, in Keywordsamyloid; ... (C), in the presence of 100 lM MPP+(D), in the presence of 200 lM MPP+(E), in the presence of 100 lM MPTP and 50 lM dopamine (F), in the presence of 200 lM MPTP and 50 lM dopamine (G), in the...

Tài liệu Báo cáo khoa học: Modulation of F0F1-ATP synthase activity by cyclophilin D regulates matrix adenine nucleotide levels pptx

... Picon-centration, which in turn could affect CYPD bindingto F0F1-ATP synthase, and decreased binding of theinhibitory protein IF 1 to ATPase. IF1 is a naturallyoccurring protein that inhibits the ... absence of cyclophilin Dor the presence of cyclosporin A led to a decrease in the extent of uncou-pler-induced depolarization. Accordingly, in substrate-energized mitochon-dria, an increase in ... relief of inhibition by cyclophilin D was evident in the form of slightly increasedrespiration rates during arsenolysis. However, the modulation of F0F1-ATPsynthase by cyclophilin D did not increase...

Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

... pheromonesto elicit a mating response in cells of the oppositemating type. Cdc42 is involved in pheromone signal-ling that eventually results in G1arrest and the for-mation of a mating projection ... formation of a matingprojection [28]. Furthermore, homologues of oxysterol-binding proteins, a family of proteins that regulatethe synthesis and transport of sterols, were found toparticipate in ... lacking histi-dine, leucine and uracil to monitor protein interactions. The unre-lated genes STE14 and UBC6 served as negative controls.Sterol homeostasis modulation by Ste20p and Cla4p M. Lin...

Tài liệu Báo cáo khoa học: Modulation of the enzymatic efﬁciency of ferredoxin-NADP(H) reductase by the amino acid volume around the catalytic site pptx

... (2004) Role of the C-terminaltyrosine of ferredoxin-nicotinamide adenine dinucleotidephosphate reductase in the electron transfer processeswith its protein partners ferredoxin and ﬂavodoxin.Biochemistry ... efﬁciency,modulating the interaction of Y308 and ⁄ or the nicotin-amide with the isoalloxazine. This type of modulation of aromatic residue interactions could be a generalstrategy occurring in enzyme ... of the wild-type FNR (thick line), L268V (thin line),C266AL268A (thick dotted line), C266A (thin dotted line), C266L(thick broken line) and C266M (thin broken line) elicited by NADP+binding,...

Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf

... ole in the regulation of cellproliferation, including SMC, by inhibiting signalling mol-ecules [ 45–47]. Caveolin-3 is the main isoform in musclecells, including SMC [30]. Caveolin-1 and caveolin-3 ... modu-lation of egr-1 gene expression (Fig. 4B).Effects of n-3 and n-6 PUFAs on the synthesis of caveolin-1 and caveolin-3 and on the location of p42/44 MAPK caveolaeCaveolins are scaffolding proteins ... effects by interfering with Ras/Raf pathways.Inhibitory effects of n-3 PUFAs on cyclin D1 gene expression and inhibition of IL-1b-induced Egr-1 gene activationEgr-1 is the product of an immediate-early...

Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: a capacitative (AC) electrical coupling model in neuroepithelium pptx

... withF-actin via b-catenin and a-catenin; b-catenin bindsto cadherin and a-catenin, which in turn interacts withF-actin (Fig. 3A) [24]. Thus b-catenin plays a pivotalrole in the regulation of cell–cell ... synchronous increases in intracellular [Ca2+], as described below.Synchronous increases in intracellular [Ca2+] and disassembly of cadherin–catenin complexesThe cytoplasmic domain of cadherin interacts ... JapanStructural organization of intracellularCa2+stores and coupling modes of Ca2+increaseChemical coupling and DC electrical couplingThe lumen of the endoplasmic reticulum (ER) iscontinuous with...

Tài liệu Báo cáo khoa học: Basis of recognition between the NarJ chaperone and the N-terminus of the NarG subunit from Escherichia coli nitrate reductase pdf

... only minor confor-mational changes upon binding the twin -arginine signalpeptide of NapA [18]. In this case, biogenesis of theNapA protein is assisted by NapF in charge of cofactorloading [19,20]. ... consists of the rapid binding of the N-terminus of the partner, regardless of whetherit is a twin -arginine signal peptide or not, followed byits release once cofactor loading and protein folding ... the binding isotherm was biphasic,with the best ﬁt obtained with a two binding sitemodel, comprising a ﬁrst site with binding stoichiome-try (n) of 0.3 ± 0.2 and a binding constant (Kd )of 3.4...

Tài liệu Báo cáo khoa học: Effect of siRNA terminal mismatches on TRBP and Dicer binding and silencing efﬁcacy pdf

... between the silencing efﬁciency of the siRNAs and theirbinding to the RNA-induced silencing complex loading complex proteinsHIV transactivating response RNA-binding protein and Dicer in H1299cytoplasmic ... cells, the dsRNA bindingproteins HIV transactivating response RNA-bindingprotein (TRBP) and Dicer recognize and bind thesiRNA and form RNA-induced silencing complex(RISC) loading complexes (RLCs) ... prominent than that of Drosophila Dicer-2, which is controlled by R2D2binding rather than actively participating in determiningwhich end to bind [18]. Future work examining internal and terminal...

Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-speciﬁc blood vascular and lymphatic endothelial cells of the mouse pdf

... pivotal roles in develop-ment and in health and disease [1]. Their propertieshave been studied by a combination of in vitro analy-ses of human primary ECs and in vivo analyses of genetically ... Yoshida and Hirotake IchiseLaboratory of Gene Expression and Regulation, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, JapanAs an indispensable component of ... immunosorting using anti-Lyve-1 antibody. Scale bars ¼ 200 nm. (B) Western blotting revealed that Lyve-1-positiveuterine ECs maintained expression of Lyve-1, Prox-1 and VEGFR-3, indicating that...

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