Tài liệu Báo cáo khoa học: Major phosphorylation of SF1 on adjacent Ser-Pro motifs enhances interaction with U2AF65 doc

... conclusion,given the strong evidence supporting the conserved SF1 ⁄ U2AF65 interaction, it is likely that the regulations of the SF1 ⁄ U2AF65 interaction by phosphorylation of SF1 on the highly conserved ... concentra-tions of mock-phosphorylated SF1f or phosphorylatedSF1f with a constant concentration of U2AF65(1.2 lm) conﬁrmed the enhancement of ternarycomplex formation upon phosphorylation ... regulator of thisstep, by inhibiting the SF1 ⁄ U2AF65 interaction upon phosphorylation of a conserved SF1 serine (Ser20)within its U2AF65 interaction domain [21].Comparison of the structure of...

Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt

... phosphory-lation in cells.Tyr18 of tau plays an important role in the interaction of tau with Fyn-SH2To determine whether the tyrosine phosphorylation status of tau itself is important for its interaction ... that this interaction isdependent on tyrosine phosphorylation of tau. A smallproportion of expressed Y18F, Y29F, Y197F, Y310Fand Y394F tau each bound to Fyn-SH2 under controlconditions, and ... these interactions of tau with thesame protein.Interactions with Fyn-SH3 are regulated by keyPXXP motifs in tauTau has been shown to bind Fyn-SH3 through spe-ciﬁc PXXP motifs, seven of which...

Tài liệu Báo cáo khoa học: Single phosphorylation of Tyr304 in the cytoplasmic tail of ephrin B2 confers high-afﬁnity and bifunctional binding to both the SH2 domain of Grb4 and the PDZ domain of the PDZ-RGS3 protein ppt

... (A)Titration with ephrinB2(301–333)-pY304.(B) Titration with ephrinB2(301–333). Theprotein concentration was 0.5 mMand thepeptide concentration was 1 mM.Ó FEBS 2004 Phosphorylation of Tyr304 on ... C0is a constant dependent on the properties of the ligand, [L]Tis the total ligand concentration, [P]istheconcentration of free SH2 protein, and Kdis the equilibriumdissociation constant. ... to lead to theregulation of the cytoskeleton of the interacting cells [1,2,6]. Phosphorylation of the well-conserved cytoplasmicdomains of B ephrins has been a subject of signiﬁcantinterest...

Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... b8-strand of one monomer into a b-sheet of the other monomer. The position of the zinc ion is indicated by acircle. (B) Schematic diagram of the dimer. Each monomer isshown with a different shade of ... site. Thus, the conformational switch of the a2-helix could be of functional relevance and beassociated with either an ‘open’ nonfunctional confor-mation or a ‘closed’ conformation adopted by ... backbone conformation of this residue (/ ⁄ wangles) falls within a ‘disallowed’ region of the Rama-chandran plot. This unusual conformation is the pre-requisite for the close proximity of the...

Tài liệu Báo cáo khoa học: Transient silencing of Plasmodium falciparum bifunctional glucose-6-phosphate dehydrogenase) 6-phosphogluconolactonase docx

... Road, London, UKMalaria is a major health hazard in tropical and sub-tropical areas around the world. In Africa alone, everyyear over a million children under the age of 5 yearsdie of malaria ... cultures of P. falciparum on the expression of para-site antioxidant defense genes at the transcription level. The highest degree of G6PD-6PGL silencing achieved was 86% at the mRNA level, with ... superoxide anionand hydrogen peroxide, respectively, showed ﬁve- andthreefold down-regulation. After 96 h of incubation inthe presence of pyrimethamine, levels of G6PD-6PGLexpression were still...

Tài liệu Báo cáo khoa học: Thermal unfolding of smooth muscle and nonmuscle tropomyosin a-homodimers with alternatively spliced exons docx

... allows study of the effects of replacement of muscle exons 1a and 2a by nonmuscleexon 1b, and comparison of Tm5a with Tm5b allowsthe effects of exchange of exon 6 to be studied.Studies of thermal ... (1971) The regulation of rabbitskeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.J Biol ... nonmuscle exon 1b. The short ﬁbroblastisoforms Tm5a and Tm5b differ internally only byexon 6. Confusingly, Tm5a has exon 6b, whereasTm5b possesses exon 6a (Fig. 1). Thus, comparison of Tm5a with...

Tài liệu Báo cáo khoa học: Crystal structure of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase complexed with an analogue of 1,3-bisphospho-D-glyceric acid Selective inhibition by structure-based design docx

... result of hydrogen bondingbetween the hydroxyl of Thr167 of the protein and theamino group of compound 8. No other inhibitor offeredsuch an interaction. For compounds 5–8, the interaction of one ... interactions with residues of the active site of T. cruzi gGAPDH.Fig. 3. gGAPDH–HOP interaction proﬁle.(A) Comparison of phosphonate and phos-phate positions of the gGAPDH–HOP com-plex with the ... inhibition kinetics studies were performed with four different concentrations of ATP (250, 350, 500 and600 lM), which correspond to 1,3-BPGA concentrations of 2–5 times the Kmvalue of this...

Tài liệu Báo cáo khoa học: "The Impact of Query Reﬁnement in the Web People Search Task" docx

... size of the target cluster: (i) rare people,mentioned in only one document (335 clusters of size 1); (ii)people that appear in two documents(92 clusters of size 2), often these documents be-long ... clusters of size >=3son dataset in the WePS corpus contains a total of 100 documents, and 10 of them belong to a Britishpolitician named James Patterson. The WePS-2corpus contains a total of ... beginning or ending with a stopword. Usingthe Stanford Named Entity Recognition Tool3weobtained the lists of persons, locations and organi-zations mentioned in each document.Additionally, we used...

Tài liệu Báo cáo khoa học: Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix docx

... mRNA fractions. Titration of thesetwo fractions indicated that Mcm2 mRNA is 4–8 timesmore abundant in the HeLa mRNA fraction than in theWI-38 fraction. In contrast, the concentration of glyceral-dehyde-3-phosphate ... anti-Mcm4(second), anti-PCNA (third) and anti-Ki67 (fourth) Ig. (B) Sectionscontaining part of the boundary of CIS (left portion) and dysplasia(CIN1 of FIGO classiﬁcation, right portion) were stained with ... TheRLF-M component of the replication licensing system formscomplexes containing all six MCM/P1 polypeptides. EMBO J. 16,3312–3319.9. Koonin, E.V. (1993) A common set of conserved motifs in a...

Tài liệu Báo cáo khoa học: The localization of FGFR3 mutations causing thanatophoric dysplasia type I differentially affects phosphorylation, processing and ubiquitylation of the receptor pptx

... termination codon had mild effects on receptor phosphorylation and glycosylation,whereas conversion of Lys650 into methionine inducedstrong constitutive phosphorylation of the native non-glycosylated ... intracellular retention. Bycontrast, TDI mutations in the ECD or disruption of the termination codon induced a much lower level of phosphorylation of only the fully glycosylated isoform,which did ... shown).The degree of constitutive phosphorylation of the mutant receptor is mutation speciﬁcBecause several FGFR3 mutations have been reportedto variably induce constitutive phosphorylation of thereceptor...

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