Tài liệu Báo cáo khoa học: Diversity and junction residues as hotspots of binding energy in an antibody neutralizing the dengue virus doc

... within and outside the CDRs [12–16]. The CDR3 loops of VH and VLcontain the residues of diversity and junction, encoded by the D segment, and the VH-D, D-JH, and VL-JLjunctions [17]. They ... 273 (2006) 34–46 ª 2005 The Authors Journal compilation ª 2005 FEBS Diversity and junction residues as hotspots of binding energy in an antibody neutralizing the dengue virus Hugues Bedouelle1, ... kinetics of inter-action with the antigen were measured by surfaceplasmon resonance. The results showed in particularthat the residues of diversity and junction constituted hotspots of binding energy, ...

Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc

... syn-thesis in vivo.To investigate the reaction mechanism of H. thermo-philus POR, we measured the EPR spectra of the enzyme in the presence of various combinations of sub-strates. Intra- and intermolecular ... coupled assay and also with respect to obtaining a deeper under-standing of the metabolism of H. thermophilus. Indeed,this would enable the kinetic analysis of the POR reac-tions in both directions. ... carboxylation of acetyl-CoA,using OGOR to reduce ferredoxin as in the coupledassay. In the presence of the reduced Fd1 and acetyl-CoA, the HE-TPP radical intermediate was formed(Figs 3F and 4B), indicating...

Tài liệu Báo cáo khoa học: Applications and trends in systems biology in biochemistry docx

... and arginaseshare the control of the fluxesthrough the pathways involvingarginine as a precurser of nitricoxide and polyaminesODE ss, sim,MCAOwn in PERLNG Refers to single timepoint of ... succeeded in describing and interpreting the effects of ethanol stress. In particular, the ratio between the kinetic constants associated withethanol production and glucoseconsumption gave the estimation ... glutamicum mutant strains showmarkedly different relative fluxcontributions (increased flux into the PPP and lysine biosynthesis,decreased flux into the TCA) at the same time as maintaining a constantsupply...

Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf

... H3, meaning that itcan direct recruitment of HBO1 both in advance of and in the wake of a transcribing polymerase. The other most likely candidates driving histoneacetylation during transcription ... major role in assisting the binding of AP-1 via cooperative binding, and creates an accessi-ble environment that also enables Sp1 and Runx1 binding [85,86,233]. Both NFAT and AP-1 have the potential ... there is an intrinsiccooperativity in the binding of transcription factors tonucleosomes [238–240]. Even in the absence of pro-tein–protein interactions, factors can assist each other’sbinding...

Tài liệu Báo cáo khoa học: miRNAs and regulation of cell signaling pptx

... repressing the indicated targets and presumably hundreds of other as yetunidentiﬁed targets. (B) miR-34a and the miR-34a -binding site in the 3¢ UTR of genes shown in (A).miRNAs and cell signaling ... Involvement of miRNAs in varioussignaling cascades. Many miRNAs are under the control of various conserved signalingpathways and in turn regulate components of these pathways, which results in the formation ... acomponent of the Hippo signaling pathway [32]. An interesting ﬁnding concerning the biogenesis of miRNAs has been reported with respect to signaling bymembers of the transforming growth factor...

Tài liệu Báo cáo khoa học: MicroRNAs and epigenetics doc

... Epigenetically-regulated miRNAs. The numbers in the binding sites’ column represent the distance (bp) between the stop codon and binding sites of seed sequences in the miRNAs. The letters ‘c’ and ‘p’ with respect ... stem-loop-shaped RNAs arecalled pre-miRNAs, and the protein complex of RNase, Drosha and DGCR8 is known as the micro-processor complex. Pre-miRNAs form a complex withexportin-5 and RAN-GTP, and are then ... deepening our understanding of diseases.Materials and methodsTyping of miRNAs by positional relationship tomRNA transcriptsInformation about the localization and strand direction of 939 miRNAs,...

Tài liệu Báo cáo khoa học: MicroRNAs and cardiovascular diseases ppt

... repolarization and the induction of PVCs and arrhythmias. On the other hand, blockingmiR-1 function with antisense oligoribonucleotides wasfound to normalize the expression of Cx43 and Kir2.1,prevent ... novel insight into the molecularunderstanding of cardiac excitability.However, considering the inherent capacity of miR-NAs to target a broad range of proteins, the linkbetween miR-1 and arrhythmia ... generated and the critical role formiR-33 in the regulation of ATP -binding cassettetransporter A1 expression and high density lipoprotein(HDL) biosynthesis was conﬁrmed in vivo [118]. In humans,...

Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt

... interesting homologs [2–4]. In humans, angiogenin (RNase 5) is an inducer of neovascularization, and plays an important role in tumor growth [5]. Eosinophil-derived neurotoxin(RNase 2) and eosinophil ... development and use of N-acylsulfonamides and sulfonimides as antagonists of nucleic acid -binding proteins.DatabaseStructural data for the two RNase A complexes are available in the Protein Data Bank ... substrates. In UpG, the binding of the guanine moiety is deterred by exocyclic O6. Closeinspection shows that the relevant subsite of RNase Ahas a negative potential and hence cannot accommo-date an...

Tài liệu Báo cáo khoa học: Active and regulatory sites of cytosolic 5¢-nucleotidase doc

... dramaticincrease in the ratio of nucleotidase to phosphotrans-ferase activity. The afﬁnity for the substrates inosine and IMP remains unaltered, and the effect of the enzyme activators ATP and Mg2+is ... transfer of phosphate can lead to phosphorylation of inosine,guanosine and a number of their analogues [2]. There-fore, in addition to being involved in regulation of purine intracellular pool, the ... the chromatographic behav-iour of recombinant wild-type enzyme in the presence of ATP and Mg2+ as an activator and Pi as an inhibitor.Figure 5 shows that the enzyme exists and functions as...

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... and hypoxanthine. (B) HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine.Table 2. Km and Vmaxvalues for Hx and G in the presence of 1 mM PRPP, determined using the DE-81 ... functions as a catalytic base [12]. However, a tight binding of N7 of the purine base and Asp137 was observed in HPRT in complex with ImmGP, indicating a role in transitionstate stabilization during ... [18].Mutations of Asp13 7in human HPRT to Asn resulted in a 290-fold and 18-fold decrease in kcatvalues fornucleotide formation with guanine and hypoxanthinebases, respectively, indicating that Asp137...

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