... thecurve-fitting procedure and describing the affinity of thesubunits for the substrate (KS1 and KS2)andforPi(KI1 and KI2), presented in Table 1 and Table 2, respectively, areindependent of the Zn2+ion ... additional possibility of enhanced or conformationally controlled product release.The crystal structure and the reaction mechanism of APase from E. coli, suggested by Kim and Wyckoff [1], aswell ... form of a dianion [9],however, the slow dissociation of Pi,andtheslowphosphorylation of Ser102 by Pi, are both in accordancewith Pibinding in form of a trianion.The crystal structure of...