Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx

... time-dependent enhance-ment of the 57-kDa band with a concomitant diminu-tion of the 38-kDa and 19-kDa bands, as analyzed bySDS ⁄ PAGE (Fig. 3A). The 38-kDa and 19-kDa bandsdid not disappear completely ... isomorphous with those of the uninhibited wild-type enzyme and of the E78Hmutant, and the structures are very similar (r.m.s.d.0.264 and 0.312 A˚for 350 Ca pairs). Crystals of theE78H ⁄ D164N mutant ... percentageintensity of the band of the 57-kDa protein in a total of intensities of the 57-kDa, 38-kDa and 19-kDa bands, where the intensity of the38-kDa protein band was corrected for the amounts of the...

Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx

... Protein–ligand and water-mediated protein–ligand hydrogen bond interactions (broken lines) that stablize the pantoate molecules in site I and site II of nPS. Oxygen atoms of pantoate and water ... changes from ) 75.20° to )171.65° and )89.25°in the A-chain and B-chain of nPS, and in the case of His37, the v1torsion angle changes from 63.34° to)67.80° and )52.11° for the A-chain and ... structure of nPS bound topantoateCocrystallization trials of nPS with pantoate wereperformed over a range of pantoate concentrations.Crystals of the nPS–pantoate complex obtained at50 mm pantoate...

Tài liệu Báo cáo khoa học: ˚ The 1.8 A crystal structure of a proteinase K-like enzyme from a psychrotroph Serratia species docx

... 177 and is involved in stabilizing strands b8 and b9.Ca4 is the weak binding site in PRK, which is formed bythe side chain of Asp260 and the carbonyl oxygen of Thr16, and stabilizes the N- and ... VPRK and SPRK have an insert of three residues. Ca3 is the so-called strong bind-ing site found in PRK and formed by the side chain of Asp200 and the carbonyl oxygen atoms of residues 175 and ... 18, 19 and 15 residues forming24, 23 and 18 interactions less than 4 A˚(SPRK, PRK and VPRK, respectively) between atoms of positively and negatively charged residues, of which 6, 3 and 5include...

Tài liệu Báo cáo khoa học: Suppressed catalytic efﬁciency of plasmin in the presence of long-chain fatty acids Identiﬁcation of kinetic parameters from continuous enzymatic assay with Monte Carlo simulation ppt

... fattyacids affected the activity of miniplasmin in the samemanner as that of plasmin; apparent activation wasseen with stearate and inhibition was seen with oleate and arachidonate (Fig. 8A). ... lLof1nM plasmin solution containingoleic acid, at concentrations (in lM) indicated by the numbers, and the absorbance at 405 nm was measured. The mean and standard deviation(dotted lines) of ... notation indicates the p-nitroaniline concentration attime tifor the kth replica with S0j). Using Pmean,i,j(mean of Pi,jk) and Pstd,i,j(standard deviation of Pi,jk) three differentapproaches...

Tài liệu Báo cáo khoa học: Multi-targeted activity of maslinic acid as an antimalarial natural compound pdf

... supported by grants from the SpanishMinistry of Education and Science (BIO2007-67885 and BIO2010-17039) and the Research Teams Consoli-dation Programme of the UCM, Research Group920267 and the Iberoamerican ... Inhibition of PLA2 activity by MA. MA and 3,4-dichloroiso-coumarin (DIC) were tested at 100 and 400 lM. The compoundswere incubated with the enzyme control and schizonts protein for15 min and 2 ... chem-ical structures with a different degree of fuzziness and thuscomplementing each other in terms of structural congenerici-ty and hopping abilities [59]. Then, the probability of anymolecule...

Tài liệu Báo cáo khoa học: Hypothalamic malonyl-CoA and CPT1c in the treatment of obesity pptx

... accompanied by a suppression in energyexpenditure. These data suggest that CPT1c canintegrate carbohydrate and lipid nutrient sensing in thebrain and is an example of an enzyme that can sense and ... Consistent with this is the closecorrelation with the hypothalamic levels of orexigenic and anorexigenic neuropeptide expression during fast-ing and refeeding. A detailed kinetic analysis of hypo-thalamic ... and nutrient availability plays an important rolein this process. Inhibition of hypothalamic fatty acid synthase by pharma-cologic or genetic means leads to an increased malonyl-CoA level and...

Tài liệu Báo cáo khoa học: Intrinsic GTPase activity of a bacterial twin-arginine translocation proofreading chaperone induced by domain swapping ppt

... assembly and export of N-oxidereductases. Biochem Soc Trans 33, 124–126.29 Zhang B, Zhang Y, Wang Z & Zheng Y (2000) Therole of Mg2+cofactor in the guanine nucleotideexchange and GTP ... co-expression of active torD restores the Tat proofreading of this enzyme and so rescues hydrogenase-2 activity in the mutant strain. RJ607 was transformed with apUNIPROM vector encoding TorD and TorDD68W,grown ... binding and hydrolysis of GTP at one site enhances the afﬁnity (or activity) towards GTP atother sites. The Hill coefﬁcient also enables an estima-tion of the number of hydrolytic sites present...

Tài liệu Báo cáo khoa học: Nucleolin/C23 mediates the antiapoptotic effect of heat shock protein 70 during oxidative stress pptx

... normal and cancerous cells [9], and ubiquitously distributed inthe nucleolus, nucleus and cytoplasm of the cell, aswell as at the cell surface [10]. Nucleolin ⁄ C23 regulatesmany aspects of DNA and ... cells transfected with the control oligo-mers and much lower at 13 and 44% in cells transfect-ed with the nucleolin ⁄ C23 antisense oligomer(Fig. 6C). Second, the activity of caspase-3 was analy-sed ... carried out in accordance with theNational Institutes of Health Guide for the Care and Use of Laboratory Animals. All efforts were made to minimizethe number of animals used and their suffering.H2O2pcDNA3.1100100...

Tài liệu Báo cáo khoa học: The cartilage-speciﬁc transcription factor Sox9 regulates AP-2e expression in chondrocytes pptx

... unlabeled wild-type oligonucleotides (lanes 3 and 8) and mutated oligonucleotides (lanes 4 and 9) wereused. Lanes 1 and 5 show the labeled oligonucleotides incubated without protein.Sox9 regulates ... expression of AP-2ein hypertrophic chondrocytes is regulated, the sequence of the AP-2e promoter was analyzed, and binding sitesfor the transcription factors AP-2a and Sox9 wereidentiﬁed. Both transcription ... activation domains of AP-2 family transcription factors. J Biol Chem 275,29701–29708.11 Williams T & Tjian R (1991) Analysis of theDNA-binding and activation properties of the humantranscription...

Tài liệu Báo cáo khoa học: Activation loop 3 and the 170 loop interact in the active conformation of coagulation factor VIIa pdf

... loop) in the active site region and the S1pocket, whereas the intramolecular connections, forinstance between the TF-binding region and the active site and between the TF-binding region and the ... activity and inhibitorsusceptibility, native and active site- inhibited G372A-FVIIa bound sTF with the same afﬁnity as the corresponding forms of FVIIa, and burial of theN-terminus of the protease ... mPEG-ButyrALD-2000 and 2 mm NaCNBH3in 50 mm Hepes,pH 7.4, containing 0.1 m NaCl and 5 mm CaCl2. Sampleswere withdrawn before initiation of the reaction and after1.5 and 5 h, and subjected to...

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