Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
... procedures
Isolation of the tyrosinase gene from
Trichoderma reesei
The tyr2 gene was amplified from genomic T. reesei DNA
with the following primers: forward, GGG GAC AAG
TTT GTA CAA AAA AGC AGG CTA TCA TGC ... TGT
CAG GTC CCT CTC G; and reverse, GGG GAC CAC
TTT GTA CAA GAA AGC TGG GTC A GT GGT GGT
GGT GGT GGT GCA GAG GAG GGA TAT GGG GAA
CGG CAA A. The PCR reaction...
... was PCR amplified from chromosomal DNA
of P. furiosus using the primers BG1279 (5¢-GCGCG
CCATGGCATCCGAGAAGATGGTTGCTATCA, sense)
and BG1297 (5¢-GCGCG
GGATCCTCATTTAAGCAT
GAAAACAACTTTGCC, antisense), ... to
homogeneity. The enzyme has a tetrameric conformation with a molecular
mass of 155 kDa. The catalytic activity of the enzyme increases up to
100 °C, and a half-life...
... within
the last 30 years. The greatest number of RIPs have
been found in the Caryophyllaceae, Sambucaceae,
Cucurbitaceae, Euphorbiaceae, Phytolaccaceae and
Poaceae [1]. Although many are potentially ... showed a
pattern of two bands of approximately 28 and 34 kDa,
related to A and B-chains respectively (Fig. 1B). The
slight differences in the migration pattern of...
... follows: HSF 1a forward,
5¢-GAAGCAGCTTGTCCAGTACACCAA-3¢; HSF 1a
reverse, 5¢-TTCCAAGAGCTGAACAAACCATTG-3¢;
HSF1b forward, 5¢-GAAGCAGCTGGTCCAGTACAC
CTC-3¢; HSF1b reverse, 5¢-GGCTGAATAAACCATGC
CAGTAGC-3¢; ... Plasmid Cloning
kit (Invitrogen) and was used as the PCR template. For
5¢-RACE, the first PCR was performed with the M13 reverse
primer (5¢-AGCGGATAACAATTTCACACAGG-3¢)asa
sense prim...
... lexicon from a
theoretical, computational and also practical
viewpoint.
INTRODUCTION
It has been traditionally assumed by
computational linguists and particularly by
designers of large natural ... whereas
the actual words are the those potential words
that are realized in this language. To give an
example, both arrival and arrivation are
potential English words, but o...
... (5¢-GT
CGTCTTGGATCCTCTCGAGTCTCAATGAGAACACA
GTCTCAAGTC-3¢). These primers contained BstEII and
BamHI sites and were used to generate a fragment that was
cloned in the same sequencing and expression ... epitopes in Aspf1 that are changed in wild-type
a- sarcin, as the response against the sera of the patients
is still lower for the latter than for the Aspf1D(7–22)
mutant...
... Nagasawa T, Ohkishi H, Kawakami B, Yamano H,
Hosono H, Tani Y & Yamada H (1982) 3-chloro-d-ala-
nine chloride-lyase (deaminating) of Pseudomonas putida
CR 1–1. Purification and characterization ... was
used for the analyses. (A) Total RNA was extracted and 20 lg RNA
was loaded in each lane and blotted as indicated in Experimental
procedures. To prove equal loading of the ex...
... and
activates a latent endoribonuclease, RNase L [3].
Activated RNase L catalyzes the degradation of viral
and cellular RNAs, including ribosomal RNA, sup-
pressing protein synthesis and viral ... mixture of A2 ¢p5 A2 ¢p5 A and A3 ¢p5 A2 ¢-
p5 A( m ⁄ z 924.6); 12, A2 ¢p5 A3 ¢p5 A( m ⁄ z 924.7); 13, putative
A2 ¢p5 A2 ¢p5 A3 ¢p5 A( m ⁄ z 1253.9); 14, mixture of A3 ¢p5 A...
... complex
than in T cells, with several peaks of nuclease hyper-
sensitivity [85]. Mast cells express GATA-2 as well as
NFAT and AP-1, and GATA-2 initiates the formation
of an additional discrete GATA-2 ... environment that also enables Sp1 and Runx1
binding [85,86,233]. Both NFAT and AP-1 have the
potential to recruit the HATs CBP and p300 which
may allow the formation o...