Tài liệu Báo cáo khoa học: Oxidation inhibits amyloid ﬁbril formation of transthyretin ppt

... and the kinetics of fibril formation of these oxidized proteins reveal the amino acid inter-actions that are critical in the onset of amyloido-genesis.The rates of amyloid fibril formation for ... tissues including amyloid fibrils [7,8].We are investigating the role of amino acid side chain oxidation in amyloid assemblies by comparing thekinetics of fibril formation of native and oxidizedproteins.Interactions ... (bottom)unoxidized tryptic peptides showing the oxidation of methionine after reaction of V30M TTR with ROS.S. D. Maleknia et al. Oxidation inhibits amyloid fibril formation of TTRFEBS Journal 273 (2006) 5400–5406...

Tài liệu Báo cáo khoa học: "Word Vectors and Two Kinds of Similarity" pptx

... SimilarityAkira Utsumi and Daisuke SuzukiDepartment of Systems EngineeringThe University of Electro-Communications1-5-1 Chofugaoka, Chofushi, Tokyo 182-8585, Japanutsumi@se.uec.ac.jp, dajie@utm.se.uec.ac.jpAbstractThis ... process-ing. Other methods use a variety of other informa-tion: cooccurrence of two words (Burgess, 1998;Schütze, 1998), occurrence of a word in the sensedefinitions of a dictionary (Kasahara et al., ... differencesin the quality of similarity among word vectorsconstructed by different methods, and if so, whatkind of similarity is involved in what kind of wordvectors. Even in the field of cognitive psychol-ogy,...

Tài liệu Báo cáo khoa học: Autophagy inhibits reactive oxygen species-mediated apoptosis via activating p38-nuclear factor-kappa B survival pathways in oridonin-treated murine ﬁbrosarcoma L929 cells doc

... death [27]. The functionalroles of the activation of these kinases are often con-troversially discussed. The ERK1 ⁄ 2 cascade isregarded to be the main method of transmission forcell proliferative ... with 500 lL of NaCl ⁄ Piand 10 mL of 70% eth-anol at 4 °C overnight; then, after washing twice withNaCl ⁄ Pi, the cells were incubated with 1 mL of PI stainingsolution (50 mgÆL)1 of PI and ... (%)Percentage of sub-G1 cells (%)Percentage of sub-G1 cells (%)Fig. 6. Effects of NF-jB and p38 on oridonin-induced L929 cells apoptosis and OS generation. The cells were incubated in the presence of absence...

Tài liệu Báo cáo khoa học: Membrane targeting and pore formation by the type III secretion system translocon pdf

... illustrating needle and translocon formation, as well as toxin secretion steps, in the T3SS of P. aeruginosa (a rep-resentative of the Ysc T3SS family). (A) Upon formation of the base rings (green), ... andresponse to pore formation The composition of the host cell membrane appears tobe a critical point for the invasion of bacteria, insertion of translocators and functionality of the pore. Targetmembrane ... Given the impor-tance of T3SS in the infection and invasion processes of a number of bacteria, these studies will likely pro-vide crucial information regarding key details of thiscomplex machinery.AcknowledgementsWork...

Tài liệu Báo cáo khoa học: MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor pdf

... because of its higher fold of upregula-tion in gastric adenocarcinoma tissues. Although theextent of upregulation of miR-23a in the four pairs of gastric tissues showed great variance because of the ... the active role of miR-23a in thegrowth promotion of malignant cells.The fundamental function of miRNAs is to regulatetheir targets by direct cleavage of mRNA or by inhibi-tion of protein synthesis. ... the effect of miR-23a on the long-term andindependent growth activity of MGC803 cells, a platecolony formation assay was performed. Comparedwith the control group, the colony number of MGC803cells...

Tài liệu Báo cáo khoa học: Molecular and cellular speciﬁcity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx

... Ollivaux C & Soyez D (2000) Dynamics of biosynthe-sis and release of crustacean hyperglycemic hormoneisoforms in the X-organ-sinus gland complex of thecrayﬁsh Orconectes limosus. Eur J ... Soyez D (2002)Enkephalinergic control of the secretory activity of neu-rons producing stereoisomers of crustacean hyperglyce-mic hormone in the eyestalk of the crayﬁsh Orconecteslimosus. J ... polymor-phism was shown to occur in isoforms of the crustacean hyperglycaemichormone (CHH) of the American lobster because a d-phenylalanyl residuewas found in position 3 of the sequence (CHH and d-Phe3...

Tài liệu Báo cáo khoa học: Substrate speciﬁcity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt

... with55 mL of the same buffer, ﬁrst alone and then with a200 mL of a decreasing gradient of ammonium sul-phate in 20 mm Tris⁄ HCl buffer (pH 8.0) with a ﬂow of 0.8 mLÆmin)1. Fractions of 2.5 ... tryptophol(17). Moreover, the rates of transformation of bothcompounds 8 and 16 in fungal cultures were substan-tially slower than the rates observed for transformation of brassinin (1) [14]. Altogether, ... correspondingbiotransformations in fungal cultures appear to suggestthat cells of L. maculans L2 ⁄ M2 produce only one type of dithiocarbamate hydrolase activity. Obviously, thisinformation is of great...

Tài liệu Báo cáo khoa học: Identiﬁcation, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx

... wascomposed of 2 lL of Moloney murine leukemia virusreverse transcriptase buffer; 0.5 lL of BSA (10 mgÆmL)1),1 lL of total RNA (1.24 lgÆlL)1), 2.5 lL of dNTP (4 mmtotal), 1.5 lL of Random ... overexpression approach of RpCAbr and RpCAtr, we cannot know the functionaleffect of changes of some key amino-acids.Origin and number of nonvertebrate CAsAlthough the bootstrap values of the deep branches ... mech-anism of carbonic anhydrase: implications of a rate-lim-iting protolysis of water. Acc Chem Res 21, 30–36.28 Tu C, Silverman DN, Forsman C, Jonsson BH & Lind-skog S (1989) Role of histidine...

Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf

... nanosecondtimescale. The lack of fast internal motions reﬂects thecrucial role of Gln50, which behaves as mechanicalpoint of support, needed for the hinging of the C-ter-minal part of helix III. This ... well-knownclass of DNA-binding domains occurring in a largefamily of transcription activators involved in thedetermination of cell development [1–3]. The tertiarystructure of the HD of rat thyroid ... values of J(0) andJ(xN), an indication of slow local motion consistentwith the presence of a hydrogen bond network thatrestricts the excursion of the Glu50 backbone. Otherrelevant details of...

Tài liệu Báo cáo khoa học: ATP-dependent modulation and autophosphorylation of rapeseed 2-Cys peroxiredoxin docx

... exhibited a mass shift of 32. Theunequivocal assignment of two oxygen atoms tothe Cys175 residue of C53S 2-Cys Prx revealed theunsuspected formation of oxyacid groups at sulfuratoms of the resolving ... concurrent displacement of theACBFig. 2. Effect of the binding of ATP ⁄ Mg2+to 2-Cys Prx on the ﬂuo-rescence emission of extrinsic and intrinsic chromophores. (A) Sen-sitivity of the extrinsic ... successive in vitro reduction ﬁ oxidation of 2-Cys Prx, we were compelled to use high concentra-tions of cumene hydroperoxide in the oxidation stepbecause high concentrations of dithiothreitol, requiredfor...

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