Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc
... Quantitative analysis of the experimental O–J–I–P
chlorophyll fluorescence induction kinetics
Apparent activation energy and origin of each kinetic step
Steve Boisvert, David Joly and Robert Carpentier
Groupe ... membranes can be modeled by
the superposition of the exponential rise to analyze
quantitatively the contribution of ea...
... DEC1 with BMAL1 and the bin ding to the E-box.
Substitution of Arg65 for Ala abolished the interaction of
DEC1 with BMAL1 and the binding of DEC1 to the E-box.
However, substitution of His57 for ... increase in the mutant
mice, the disruption of DEC1 might be compensated by
DEC2. Further investigation is required to clarify the
functions of DEC1 and DEC2.
Th...
... calculation of the fraction (f
a
) and the effective
quenching constant (K
Q
) for the most accessible class of
Trp chromophores [16].
The pH dependence of the Trp fluorescence was studied
in the pH ... spectroscopy. The intensity of
Trp fluorescence of purified HpPex5p increased by 25%
upon shifting the pH from pH 6.0 to pH 7.2. Together with
the results o...
... (Met1)-ONC
(M23L) by the addition of AAP, as described in the
Materials and methods. Completion of the hydrolysis was
confirmed by the disappearance of the (Met1)-ONC
(M23L) signal in the MALDI-TOF mass spectra ... analyzed by MALDI-TOF MS. The
addition of the protease is considered the zero time point.
The relative intensities of the peaks from the mass spe...
... gels of each experimental
condition were built and then compared; spots were quanti-
fied on the basis of their relative volume (spot volume nor-
malized to the sum of the volumes of all the representative
spots) ... apoptosis and induces
the synthesis of antioxidants [22].
Alterations in mitochondrial proteins, on the other
hand, are specifically linked to one of...
... the titrant. The active site
concentration of wild-type BuChE was 0.16 ± 0.01 lm,
and that of the D70G mutant was 0.196 ± 0.02 lm.
Effects of ligands on steady-state kinetics
The effects of the ... free of albumin and any other AAAs. It was
found that there was no fundamental difference in the
mechanisms of inhibition and activation for either the
AAA or es...
... of MCL and the effect of ligand binding on it,
the far-UV CD spectra of MCL in the native state as well as
in the presence of CuTCPP and CuTMPyP were analysed
by the
CDSSTR
program using the routines ... investigated the
interaction of several water-soluble porphyrins with MCL.
The thermodynamic forces governing the interaction of
some of the porphyrins h...
... ÔStains-
AllÕ analysis of Fig. 3 and the 1D i mmunoblotting of Fig. 4.
As the full-length Dp427 isoform of dystrophin does not
enter the second dimension of conventional 2D gels, the
expression level of ... CSQ), and
calmodulin (CAM), respectively. The pH values of the fi rst dimension
gel system and molecular mass standards (in kDa) of the second
dimension...
... made in the
pathway dissociate from PGI and equilibrate with the
pool of Fru6P in the aqueous cytoplasm, then there
would be no channeling. When there is no channeling,
the result of the competition ... Fru6P, but the signa-
ture of that channeling would be lost because Fru6P
was apparently not channeled.
The data in Table 3 show the method for and the
results of...
... bond, linkage of the active
site Ser of the protease to the carboxyl group of P
1
by an
ester bond and insertion of the N-terminal part of the RCL
as strand 4 in b-sheet A (s4A) of the serpin. ... behaviour of the serpin.
Complex formation between serpins and their cognate
proteases is fuelled by the thermodynamic properties of the
serpin. Accordingly, in...