Tài liệu Báo cáo khoa học: Sensor of phospholipids inStreptomycesphospholipase D pdf
Tài liệu Báo cáo khoa học: Sensor of phospholipids inStreptomycesphospholipase D pdf
... structure of TH-2PLD is sho-wn using the Swiss-PDB viewer and isbased on the crystal structure of PMFPLD.The identified key residues are indicated inred. The N-terminal and C-terminal HKDmotifs ... 000, 53 500, 36 100 and 29 500 Da). Thesamples were loaded on a 10% acrylamidegel. The arrowhead indicates the position of the purified PLDs. Sensor of phospholipids in PLD Y. Uesugi et al.2674 ... PLD.Preparation of vesiclesAn aliquot of phospholipids dissolved in chloroform wasevaporated and further dried in vacuum for at least 3 h.The lipids were hydrated to a concentration of 10 mm inphosphate-buffered...
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Tài liệu Báo cáo khoa học: Motion of the Ca2+ -pump captured ppt
... were of reagentgrade. SR was purified and washed with DOC as describedpreviously [13,14]. The purified SR and DOC-washed SRwere stored in liquid nitrogen. The protein concentration inSR was determined ... and wereadsorbed onto the mica surface as lipid bilayer mem-branes with a thickness of 5.1 ± 0.6 nm [mean ±standard deviation (SD), N = 23]. These DOC-treatedSR membranes immobilized on the ... captured the motion of the SERCA molecule (purple dot) embedded in thesingle lipid bilayer on mica (Fig. 1A, Movie S1). Up–down motions and shape changes between taller (com-pacted) and shorter...
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Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx
... 5,5¢-dithio-bis(2-nitrobenzoic acid)and NADPH at 340 nm. GSSG was determined by thesame method in the presence of 2-vinylpyridine and reducedGSH was calculated from the difference between total glu-tathione and GSSG ... GdCl3attenuatedthe imbalanced vascular stress gene expression inducedby sepsis [17]. In the present study, the depletion of KCs was confirmed by dramatically reduced expression of the KC marker gene CD163.In ... manneras GdCl3. Four treatment groups were studied: (a) vehicle-treated sham (sham); (b) GdCl3-treated sham (GdCl3); (c)vehicle-treated CLP (CLP); and (d) GdCl3-treated CLP(GdCl3+...
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Tài liệu Báo cáo khoa học: Mechanisms of amyloid fibril formation – focus on domain-swapping doc
... code: 1N9J); (B) stefin B monomer (Protein Data Bankcode: 1stf) and domain-swapped dimer (Protein Data Bank code: 2oct); and (C) proposed mechanism of the building up of amyloid fibrilsobtained ... studies,cellular and animal models, and clinical studies. Inaddition to providing a basic understanding of the pro-cesses of protein folding and aggregation, such datahelp towards translational ... amyloid-preceding confor-mation; this remains to be discussed. As the number of studies grow, insight will be provided as to how spe-cific (or indeed how random, directed or evolved) theamyloid...
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Tài liệu Báo cáo khoa học: Modulation of a-synuclein aggregation by dopamine in the presence of MPTP and its metabolite pptx
... intensity indeed denoted the formation of highermolecular mass aggregates, SDS ⁄ PAGE and immuno-blotting were carried out according to the proceduredescribed in Materials and methods. a-Synuclein ... patients of idiopathic Parkinson’s disease anddevelop the same therapy-related complications. Post-mortem analysis of brains of patients with MPTP-induced Parkinson’s disease has disclosed importantsimilarities ... Education and Research (NIPER), S.A.S. Nagar, IndiaIntroductionThe inability of the cell to degrade various stable mis-folded proteins leads to the formation of aggregatesand inclusion bodies...
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Tài liệu Báo cáo khoa học: Modulation of F0F1-ATP synthase activity by cyclophilin D regulates matrix adenine nucleotide levels pptx
... andhydrolysis have been estimated sequentially from the samemitochondria: first mitochondria were energized, a smallamount of uncoupler was added, then ADP was added, andATP efflux was recorded; ... recorded; 150 s later, 1 lm of SF 6847 wasadded, and ATP influx was recorded; after 150 s, alamethi-cin was added, and ATP hydrolysis by the F0F1-ATP syn-thase was recorded). Fminand Fmaxwere ... subsequentlyrecorded as detailed above. For conversion of calibrated free[Mg2+] to free ADP and ATP appearing in the medium, theinitial values of total ADP and Mg2+was consideredbecause free [ADP] and...
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Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx
... of individuals, mirroring a highlybalanced degree of co-evolution of H. pylori andhumans. Studies of host–bacterial interactions and vir-ulence factors CagA and the T4SS have provided uswith ... as useful in vivo models tostudy H. pylori-induced pathology. However, each ani-mal model has distinct advantages and disadvantages,and therefore only can be considered as complemen-tary ... articles[1–3,5] (more details are also provided in Doc. S1).During the last two decades, the cellular and molecularmechanisms acquired by H. pylori to undermine hostdefences have been investigated intensively...
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Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf
... oleate, inhibited cell proliferation and induced cell death; (b)stearate-induced cell death had the characteristics of endoplasmic reticulumstress-mediated and mitochondrial-mediated apoptosis; ... harvested, and lipids wereextracted twice with CHCl3⁄ MeOH ⁄ ddH2O (1 : 1 : 0.9).Lipid extracts were dried under a nitrogen stream, redis-solved in chloroform ⁄ methanol (2 : 1), and separated ... persample were counted, and BrdU-positive cells wereexpressed as a percentage of the total cell number.Detection of lipid accumulationLipid droplet accumulation was detected by Nile red stain-ing...
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Tài liệu Báo cáo khoa học: Kinetics of dextran-independent a-(1 fi 3)-glucan synthesis by Streptococcus sobrinus glucosyltransferase I pdf
... increasing DPmay be a common feature of glucansucrase.Additional studies are required to elucidate the dex-tran-dependent synthesis of glucan. As the affinity of the GBd of GTF-I for dextran is ... procedures. Filled and open circles indi-cate the GTF activity of GSGB and GS, respectively.ABFig. 3. Saccharide production kinetics during the dextran-indepen-dent GTF-I reaction. (A) and ... analyzed with HPLC, but no peak cor-responding to nigerooligosaccharides was detected(data not shown). In addition, TLC detected majorspots of fructose, glucose, and leucrose; immobile spotsand...
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Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc
... CAD⁄ICADDFF is composed of two subunits, a 40 kDa caspase-activated DNase (CAD) ⁄ DFF40 and a 45 kDa inhibi-tor of CAD (ICAD ⁄ DFF45), the complex of which isan inactive form. During apoptosis, ... MAPK185183Degradation125p53BaxAkt473Caspase-3ERK1708769Apaf-1ApoptosomeCaspase-9Cleavedcaspase-3Endo GAIFDimerizationPARPDNA fragmentationNucleusCytoplasmCADICAD-SCADCADICADRibosomeCADCADICADCADICADCleavedPARPNuclear exportResponse to DNA damageBaxPP62184Bcl-xLBaxPhosphorylated ... inthe repair of DNA damage and the induction of DNAdegradation leading to cell death; therefore, the phos-phorylated form of H2AX (cH2AX) is a sensitivemarker for DNA double-stranded breaks [92,93].It...
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Từ khóa: tài liệu báo cáo nghiên cứu khoa họctài liệu về báo cáo khoa họcbáo cáo khoa học tài chính côngbáo cáo khoa học số loài quý hiếm tại vườn quốc gia ba bểtai lieu bao cao thuc tap khoa co khitai lieu bao cao thuc tap tai khoa duoc benh vienBáo cáo quy trình mua hàng CT CP Công Nghệ NPVđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThơ nôm tứ tuyệt trào phúng hồ xuân hươngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXChuong 2 nhận dạng rui roBT Tieng anh 6 UNIT 2Nguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)BÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘI