Tài liệu Báo cáo khoa học: Compartmentalization and in vivo insulin-induced translocation of the insulin-signaling inhibitor Grb14 in rat liver pptx

... domain of the IR in complex with the PIR–BPS of Grb14 and of the Grb14 SH2 domain have allowed us to propose amodel for the Grb14 IR interaction. The PIR–BPSbinds as a pseudosubstrate inhibitor ... translocation of Grb14 in rat liver FEBS Journal 275 (2008) 4363–4377 ª 2008 The Authors Journal compilation ª 2008 FEBS 4377 Compartmentalization and in vivo insulin-induced translocation of the insulin-signaling ... domain of the IR in complex with the PIR ⁄ BPS domain of Grb14, Depetris et al. [8] have shown that Grb14 binds as apseudosubstrate inhibitor to the peptide-binding groove of the kinase and...

Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc

... coupledassay. In the presence of the reduced Fd1 and acetyl-CoA, the HE-TPP radical intermediate was formed(Figs 3F and 4B), indicating the intermediacy of the HE-TPP radical in both the oxidative and ... under-standing of the metabolism of H. thermophilus. Indeed,this would enable the kinetic analysis of the POR reac-tions in both directions. In particular, the reaction rateunder physiological intracellular ... CO2 and the carboxylgroup of 2-oxoglutarate [44], and therefore interfereswith the detection of [14C] pyruvate. Instead, the rate of pyruvate formation was determined by monitoring the LDH-coupled...

Tài liệu Báo cáo khoa học: Diversity and junction residues as hotspots of binding energy in an antibody neutralizing the dengue virus doc

... the numbering of the amino-acid sequences of immunoglobulins and the definition of their CDR loops, as described from 1997 [13]. The number-ing and definition of the L-CDR3 and H-CDR3 loops of mAb4E11 ... on anti-bodies and their genes, and performed a systematicscanning of the CDR3 loops of mAb4E11 by mutagen-esis of their residues into alanine (Ala scanning). The affinities of the purified mutant ... along the x axis, and the optical signal A405 in the indirect ELISA is givenalong the y axis. This signal is proportional to the concentration of free Fab4E11-H6 in the binding reaction. The...

Tài liệu Báo cáo khóa học: Trichostatin A reduces hormone-induced transcription of the MMTV promoter and has pleiotropic effects on its chromatin structure pptx

... containingminichromosomesTreatment of oocytes with deacetylase inhibitors such asTSA may change the bulk acetylation pattern of histones, and in this way alter the structure of chromatin incorpor-ating ... with the topologychanges resulting from the loss of 1.5 superhelical turns of the DNA over the whole construct, suggest that the chromatin is indeed more relaxed in the absence of hormone. Interestingly, ... TSAadministration has on chromatin structure and on geneexpression.Materials and methodsDNA and plasmidsConstruction of the MMTV reporter and the plasmid for in vitro transcription of rat GR...

Tài liệu Báo cáo khoa học: Applications and trends in systems biology in biochemistry docx

... acid,argininecatabolismto NO and polyaminesNG, aortaendothelialcellsLow afﬁnity transporter and arginaseshare the control of the ﬂuxesthrough the pathways involvingarginine as a precurser of nitricoxide and polyaminesODE ... computationalmodeling. Here, computational modeling does notrefer to statistical models or models of data miningbut rather to a mathematical or ’virtual’ representation of the living system of interest in the ... succeeded in describing and interpreting the effects of ethanol stress. In particular, the ratio between the kinetic constants associated withethanol production and glucoseconsumption gave the estimation...

Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf

... [238–240]. Even in the absence of pro-tein–protein interactions, factors can assist each other’sbinding to DNA within nucleosomes. This principle isillustrated in the model shown in Fig. 7A. In this ... varia-tion and complexity in the number of nucleosomes dis-rupted, the histone composition of the remainingnucleosomes, and the number and nature of proteincomplexes they interact with.Much has ... Phosphorylation of histone H3S10 by Pim1 kinase enables the recruitment of both MOF and P-TEFb via interactions involving the adaptor protein 14-3-3 and the bromodomain pro-tein BRD4 which is...

Tài liệu Báo cáo khoa học: miRNAs and regulation of cell signaling pptx

... repressing the indicated targets and presumably hundreds of other as yetunidentiﬁed targets. (B) miR-34a and the miR-34a-binding site in the 3¢ UTR of genes shown in (A).miRNAs and cell signaling ... fac-tor induced the expression of miR-221 and miR-222 in PC12 cells, and that this induction is dependent onsustained activation of the ERK1 ⁄ 2 pathway [20].Furthermore, the induction of miR-34a ... inducedby the action of conserved signaling pathways but, in turn, the induced miRNAs regulate these pathways byrepressing the expression of components of the signal-ing pathways and, in some cases,...

Tài liệu Báo cáo khoa học: MicroRNAs and epigenetics doc

... (RISC)-loading complex(RLC) in an ATP-dependent manner [20]. Next, onestrand (the passenger strand) of the miRNA isremoved from the RLC, whereas the other strand (the guide strand) remains in the ... both the promoter of the host gene and the promoter in the latter CpG island. The methyla-tion levels of the CpG island are inversely correlatedwith mature miR-34b ⁄ c expression levels in variouscancers ... binding sites of seed sequences in the miRNAs. The letters ‘c’ and ‘p’ with respect to miRNA binding site numbers indicate that the miRNA binding sites are the ‘conserved region’ and ‘poorly conserved...

Tài liệu Báo cáo khoa học: MicroRNAs and cardiovascular diseases ppt

... proteins involved in fibrosis, includingmultiple collagens, fibrillins and elastin [75]. miR-29 isdramatically repressed in the border zone flanking the infracted area in the mouse model of MI. ... novel insight into the molecularunderstanding of cardiac excitability.However, considering the inherent capacity of miR-NAs to target a broad range of proteins, the linkbetween miR-1 and arrhythmia ... decrease in ATP-binding cassette transporter A1because of the increased production of miR-33b from the insulin-induced induction of SREBP1c. Although itis impossible to prove this in animal...

Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt

... [46]. The similarity in the Kmvalues is attributable to the uracil moiety binding in the same fashion [38], which could trigger the initialbinding of both substrates. In UpG, the binding of the guanine ... studies of the structure and binding sites of enzymes. 8. Inhibitor binding toribonucleases. J Mol Biol 45, 491–511.33 Ui N (1971) Isoelectric points and conformation of proteins. I. Effect of urea ... regarding a new class of surrogates for the phosphoryl group.Results and DiscussionSulfonimides as inhibitors of RNase AWe began by determining the ability of three backboneanalogs of RNA to inhibit...

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