Tài liệu Báo cáo khoa học: Dimers of light-harvesting complex 2 from Rhodobacter sphaeroides characterized in reconstituted 2D crystals with atomic force microscopy docx

... 118, 22 6 23 5.AFM study on LH2 2D crystal L N. Liu et al.3166 FEBS Journal 27 5 (20 08) 3157–3166 ª 20 08 The Authors Journal compilation ª 20 08 FEBS Dimers of light-harvesting complex 2 from Rhodobacter ... protruding LH2rings, approximately 120 ° (Fig. 2A) and 90° (Fig. 2B),indicated two types of zig-zag lattice. The inset of Fig. 2A shows a high-magniﬁcation image of LH2rings embedded in the 2D crystals. ... like-protein photosyn-thetic complexes, we report an atomic force microscopy investigation onartiﬁcially created 2D crystals of the peripheral photosynthetic light-har-vesting complexes 2 (LH2’s) from...

Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: a coupled oscillator-based mechanism in smooth muscle ppt

... typically in the range 2 3 mm [26 ].Finding experimental evidence that electrical cou-pling is the key ‘spring’ interlinking the Ca 2+ storeshas involved repeating the decoupling experiment of Fig. ... organisms [20 22 ]. Ca 2+ waves areconsidered to be generated by the release of Ca 2+ from a dominant store, triggering Ca 2+ -induced Ca 2+ release from adjacent stores, and the continuation of this ... 80 100 120 140 160 180–65–60–55–50–45V (mV)V (mV)V (mV)1 2 3 2 min10 s3 20 mV 2 13 2 150 100 150 20 0 25 0 300–65–60–55Time (min)Time (min)Time (min)0 20 40 60 80 100 120 140...

Tài liệu Báo cáo khoa học: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area ppt

... of ExperimentalMedical Science, BMC, C11, SE -22 1 84Lund, SwedenFax: +46 4 622 24 022 Tel: +46 4 622 28581E-mail: cecilia.holm@med.lu.se(Received 10 March 20 09, revised 17 May 20 09, accepted 25 ... al.47 62 FEBS Journal 27 6 (20 09) 47 52 47 62 ª 20 09 The Authors Journal compilation ª 20 09 FEBSPhosphorylation of hormone-sensitive lipase by proteinkinase A in vitro promotes an increase in its ... lipoproteinlipase; PKA, protein kinase A; TO, triolein.47 52 FEBS Journal 27 6 (20 09) 47 52 47 62 ª 20 09 The Authors Journal compilation ª 20 09 FEBSlevels of cAMP, which causes the catalytic subunits of PKA...

Tài liệu Báo cáo khoa học: Roles of the human Rad51 L1 and L2 loops in DNA binding doc

... residue,replacing the Asp231, Ser233, or Gly236 residue, upon DNA binding. Thealanine replacement of phenylalanine at position 27 9 (Phe279) within theL2 loop did not affect the DNA-binding ability of ... and L2 loopsFEBS Journal 27 3 (20 06) 3148–3159 ª 20 06 The Authors Journal compilation ª 20 06 FEBS 3157recombination from the structure of a RAD51-BRCA2 complex. Nature 420 , 28 7 29 3. 22 Kinebuchi ... is involved in the subunit–subunit interaction,because of its exposure to solvent upon the disso-Y232W F279WY232A F279AWTD231W G236WS233W1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 ...

Tài liệu Báo cáo khoa học: Oligomerization of the Mg2+-transport proteins Alr1p and Alr2p in yeast plasma membrane pptx

... Oligomerization of Alr1p and Alr2pFEBS Journal 27 3 (20 06) 423 6– 424 9 ª 20 06 The Authors Journal compilation ª 20 06 FEBS 424 9Oligomerization of the Mg 2+ -transport proteins Alr1p andAlr2p in yeast ... without Mg 2+ and inoculated in equal density into media containing 5, 25 , 100, or 1000 lM Mg 2+ . Cells were incubated at 28 °C with shaking. Growth was followed by measuring the D600for 24 h.Oligomerization ... selected. Correct insertion of the cas-sette was veriﬁed by PCR analysis using primers ALR2-up in combination with HIS3-r, resulting in a 770-bpfragment indicating correct insertion of the HIS3...

Tài liệu Báo cáo khoa học: Transcription of individual tRNA1Gly genes from within a multigene family is regulated by transcription factor TFIIIB pdf

... differentphases of the DNA helix allow TFIIIB binding in anactive state (i.e. capable of interacting with TFIIIC tostabilize binding) in the case of tRNAGly1-1 and in aninactive state (incapable of ... Journal 27 2 (20 05) 5191– 520 5 ª 20 05 FEBS 5195these complexes were dissociated in the presence of heparin in both cases (lane 4). This binding was viadirect interactions of the TBP component of TFIIIB with ... Parthasarthy and K. P. Gopinathan Regulation of pol III transcriptionFEBS Journal 27 2 (20 05) 5191– 520 5 ª 20 05 FEBS 520 5Transcription of individual tRNAGly1genes from within amultigene family...

Tài liệu Báo cáo khóa học: A multi-protein complex containing cold shock domain (Y-box) and polypyrimidine tract binding proteins forms on the vascular endothelial growth factor mRNA Potential role in mRNA stabilization pptx

... may interact. Consistent with this, CSD proteinshave been shown to interact with a number of partnerproteins in solution [22 ,23 ].Involvement of the VEGF 3¢-UTR in binding CSDand PTB proteinsAs ... proteins: anessential role for the cold shock domain. J. Biol. Chem. 27 0, 28 297 28 303. 62. Tillmar, L. & Welsh, N. (20 02) Hypoxia may increase rat insulinmRNA levels by promoting binding of ... site 2) contained aprotein of 50 kDa, consistent with the size of the CSDprotein, dbpB (also known as YB-1/p50) [ 42] . Additionalproteins in the complex had sizes of 60, 27 and 12 kDa. Thesingle...

Tài liệu Báo cáo khoa học: Cleavage of nonphenolic b-1 diarylpropane lignin model dimers by manganese peroxidase from Phanerochaete chrysosporium Evidence for a hydrogen abstraction mechanism docx

... August 20 02, revised 31 October 20 02, accepted 21 November 20 02) Eur. J. Biochem. 27 0, 28 4 29 2 (20 03) Ó FEBS 20 03 doi:10.1046/j.14 32- 1033 .20 03.03386.xmechanisms. The products formed from the ... at 28 °Cfor5min in 1 mL of 20 mMsuccinate, pH 3.0, containing thesubstrate (180 lM) and enzyme (5 lg). The reactions wereinitiated by the addition of 100 lMH 2 O 2 and wereÓ FEBS 20 03 ... lignin models via formation of aphenoxy radical [9 ,24 27 ]. MnP is also capable of oxidizingnonphenolic lignin model dimers and veratryl alcohol, in thepresence of a radical mediator [28 –30]....

Tài liệu Báo cáo khoa học: Motion of the Ca2+ -pump captured ppt

... 10 nM free Ca 2+ (N = 54), (C) 10 nM free Ca 2+ and 10 lM TG, after 30 min incuba-tion (N = 82) . The lines are Gaussian ﬁts of the height differencedata.0 4 8 121 620 2 428 323 60 20 406080100FrequencyTime ... Motion of the Ca 2+ -pump capturedFEBS Journal 27 8 (20 11) 3 025 –3031 ª 20 11 The Authors Journal compilation ª 20 11 FEBS 3 029 studies [27 ,28 ], in which the E2P*-ATP, E2-ATP andCa2E1–P-ADP structures ... height of 5.5 nm from the membrane surface. (B, C) Single line scan (2D observation) FSAFM images of SERCA wereobtained in the presence of 100 lM free Ca 2+ and in the presence of 10 nM (B) and...

Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx

... CLPCYP1A1β-actinβ-actinβ-actinβ-actinCYP2B1CYP2E1CYP1A2Fig. 2. Effects of KCs on hepatic CYP1A1, 1A2, 2B1 and 2E1 protein expression levels 24 h after CLP. Rats were pretreated intravenously with ... largeamounts of ROS during sepsis [24 ]. Interestingly, phos-phorylation of CYP1A1 ⁄ 1A2 occurred at 24 h afterSham GdCl3CLP GdCl3 + CLPCYP1A1β-actinβ-actinβ-actinβ-actinCYP2B1CYP2E1CYP1A2Fig. ... glycine. Am J Phys-iol Gastrointest Liver Physiol 28 1, G200 20 7. 26 Hakkola J, Hu Y & Ingelman-Sundberg M (20 03)Mechanisms of down-regulation of CYP2E1 expressionby in ammatory cytokines in...

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