... compilation ª 2008 FEBS
Calcium-independent phospholipase A
2
mediates
proliferation of human promonocytic U937 cells
Marı
´a
A. Balboa, Rebeca Pe
´
rez and Jesu
´
s Balsinde
Institute of Molecular Biology ... mediate the effect of iPLA
2
on U937 cell proliferation.
Discussion
In this study, we demonstrate that iPLA
2
-VIA is
required for the proliferation of hu...
... inhibitory
effect on human ACMSD. The presence of the metal-
locofactor in human ACMSD might also be inferred
by our study on the enzyme pH dependence, showing
that the increase of the concentrations of succinate
and ... biochemical characterization of human
ACMSD.
Results
Cloning of ACMSD transcripts
The complete coding sequence of human ACMSD
was obtained from reverse-tr...
... because of its higher fold of upregula-
tion in gastric adenocarcinoma tissues. Although the
extent of upregulation of miR-23a in the four pairs
of gastric tissues showed great variance because of
the ... the active role of miR-23a in the
growth promotion of malignant cells.
The fundamental function of miRNAs is to regulate
their targets by direct cleavage of mRNA or by...
... D-CHH cells (red), D-VIH cells (green) and D -cells produc-
ing both
D-isomers (orange). (F) Enlarged view of the three cell types showing the variations of coloration for D -cells: D-CHH cells ... addition to cells containing exclusively
l-isomers of CHH or VIH, three types of cells were
found, producing: (a) d-Phe3 CHH (d-CHH cells) , (b)
d-Trp4 VIH (d-VIH cells) or (c)...
... Department of Pharmacy, University of Tromsø, Norway
2 Department of Medical Biochemistry, University of Tromsø, Norway
3 School of Pharmacy, University of Oslo, Norway
4 Department of Tumor Biology, ... number of via-
ble cells were observed for both cell lines attached to
plastic. However, when the cells were attached to
monomeric 2D collagen, the number of viable cel...
... with
55 mL of the same buffer, first alone and then with a
200 mL of a decreasing gradient of ammonium sul-
phate in 20 mm Tris⁄ HCl buffer (pH 8.0) with a flow
of 0.8 mLÆmin
)1
. Fractions of 2.5 ... appear to suggest
that cells of L. maculans L2 ⁄ M2 produce only one type
of dithiocarbamate hydrolase activity. Obviously, this
information is of great importance to the design...
... was
composed of 2 lL of Moloney murine leukemia virus
reverse transcriptase buffer; 0.5 lL of BSA (10 mgÆmL
)1
),
1 lL of total RNA (1.24 lgÆlL
)1
), 2.5 lL of dNTP (4 mm
total), 1.5 lL of Random ... overexpression approach of
RpCAbr and RpCAtr, we cannot know the functional
effect of changes of some key amino-acids.
Origin and number of nonvertebrate CAs
Although the boot...
... of support, needed for the hinging of the C-ter-
minal part of helix III. This relative rigidity of resi-
due 50 is also relevant to biological function, and has
been long recognized as one of ... well-known
class of DNA-binding domains occurring in a large
family of transcription activators involved in the
determination of cell development [1–3]. The tertiary
structure of t...
... cysteine.
Results
The concerted action of nucleotides and Mg
2+
modulates the peroxidase activity of 2-Cys Prx
A thorough inspection of the available X-ray structure
of human 2-Cys Prx (PDB entry: 1QMV) ... solution [23]. In
the absence of perturbants, the predominant form of
A
C
B
D
Fig. 1. Effect of nucleotides ⁄ Me
2+
on the functions of 2-Cys Prx. (A) Concerted action o...
... Pro, Val or Lys, c-carboxylation
of Glu, glycosylation of Ser or Thr, bromination of
Trp, sulfation of Tyr, cyclization of N-terminal Gln,
and epimerization of several different residues. It ... structure of conomarphin
was similar to those of other conotoxin cDNAs. The
cDNA-encoded precursor of conomarphin consisted of
a conserved M-superfamily signal peptide of 25 resi...