Tài liệu Báo cáo khoa học: KCNE4 can co-associate with the IKs (KCNQ1–KCNE1) channel complex ppt

... used to establish that KCNE4 directly associates with KCNQ1, and can co-associate together with KCNE1 in the same KCNQ1 complex to form a ‘triple subunit’ complex (KCNE1–KCNQ1 KCNE4) . We also used ... interaction of KCNE4 with KCNQ1. This hypothesis was tested byexamining whether KCNE4 forms protein complexes with KCNQ1. KCNE4 and KCNQ1 were transientlyco-expressed in COS-M6 cells, the protein complexeswere ... ice with shaking. The bioti-nylation solution was removed and the reaction wasquenched by washing twice with DPBS with 100 mm gly-cine. The cells were then incubated in the same DPBS with glycine...

Tài liệu Báo cáo khoa học: Bone morphogenetic proteins in the early development of zebraﬁsh pptx

... to the type I and type II receptors on the cellsurface. These receptors are serine ⁄ threonine kinasereceptors with a single transmembrane domain. Bind-ing of the ligand to the receptor complex ... complex induces the type II receptor to phosphorylate the type Ireceptor, which then leads to activation of the type Ireceptor. The signal is passed on to the substrates of the type I receptor ... differentcell types [18]. The morphogen concentration is highin the ventral and lower in the dorsal domain. The steepness of the gradient is determined by how muchBMP there is available. The different...

Tài liệu Báo cáo khoa học: "A COMPUTATIONAL VIEW OF THE COGNITIVE SEMANTICS OF SPATIAL PREPOSITIONS*" ppt

... of the long desk is a chair. Another chair is to the left of the long desk. The chair in front of the desk is near the short desk." OTHER APPROACHES AND CLOSING REMARKS Nearly all the ... Intuitively, the closer the "stool" is to the region of space defined by the projection of the desk's dimensions into this space, the more the spatial arrangement conforms to the prototypical ... of the LO relative to a particular side of the RO I ; lastly (c) predicates proximity and a relative positioning of the LO with respect to the RO, with the addi- tional anti-alignment of the...

Tài liệu Báo cáo khoa học: "Harnessing NLP Techniques in the Processes of Multilingual Content Management" pptx

... help the users to discern essential information with unparalleled clarity. The “Technologies behind the system” chapter describes the implementation and the integration approach of the core ... managing dynamic content-driven websites with a linguistic platform. The platform enriches the content of these websites with revealing details and reduces the manual work of classification editors ... system and the benefits of using it. The chapter “Evaluation” briefly discusses the user evaluation of the new system. The last chapter “Conclusion and Future Work” summarises the main achievements...

Tài liệu Báo cáo khoa học: An alternative isomerohydrolase in the retinal Muller cells of a cone-dominant species doc

... thetase to esterify the 13cROL generated in the reac-tion. Therefore, 13cROL is accumulated in the absenceof LRAT in the reaction (Fig. S3B).Under the in vitro assay conditions of the ... washes with NaCl ⁄ Tris with Tween 20, the membrane was then incubated for 1.5 h with a1 : 25 000 dilution of anti-mouse IgG conjugated with Dy-Light 549 and anti-rabbit IgG conjugated with DyLight649 ... the entire thicknessof the inner retina. The Mu¨ller cell constitutes an ana-tomical link between the retinal neurones and supportstheir activities by exchanging molecules between the other...

Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc

... mitochondria during apoptosis. They induce the permeabilization of the mitochondrial membrane with the release of cytochrome c and the formation of the apoptosome [38]. The major pro-apoptotic proteinBax ... mitochondriaand then translocate to the nucleus where they induce DNA fragmentation in a caspase-independent manner. Whether apoptosis is inducedor not depends on the activation balance of these proteins. ... lacks the C-terminal 63 residues of ICAD [27]. This shortform also dimerizes with CAD, and partially main-tains the function of the inhibitor and chaperone[28]. ICAD-S cannot translocate to the...

Tài liệu Báo cáo khoa học: Basis of recognition between the NarJ chaperone and the N-terminus of the NarG subunit from Escherichia coli nitrate reductase pdf

... enthalpy is the sum of twoterms: the reaction enthalpy, independent of the bufferused in the experiment, and another term representing the contribution of the proton ionization of the buffer,which ... HCl, 1 mM MgCl2, 100 mM NaCl. The upper panels show the raw data for the heat effect during the titrations; the lowerpanels are the binding isotherms.Table 2. Thermodynamic parameters of NarG(1–15) ... and the lifespan of the complex by 10-fold, as judged by koff. The physiologicalchaperone cycle probably consists of the rapid bindingof the N-terminus of the partner, regardless of whetherit...

Tài liệu Báo cáo khoa học: An autoinhibitory effect of the homothorax domain of Meis2 ppt

... another factor or othersignals are required to fully derepress Meis2d. The Meis2e splice variant retains the Hth domain,but lacks both the HD and the AD. It could thereforeinteract with Pbx, but ... lines.It may therefore represent a signiﬁcant proportion of the functional Meis3 protein. However, further workwill be required to determine the relative levels of the proteins encoded by these two ... such as the E2a–Pbx protein. Additionally, the interaction of Meis family proteins with a Pbx pro-tein allows for recruitment of the Meis protein to aDNA-bound Pbx–Hox complex, without the need...

Tài liệu Báo cáo khoa học: An allosteric DNAzyme with dual RNA-cleaving and DNA-cleaving activities doc

... Weprepared the 46-nucleotide DNAzyme with DNA-cleaving activity(PL DNAzyme), and then grafted the deoxyribonucleotide residues froman 8–17 variant DNAzyme into the region of stem–loop I and the catalyticcore ... ssDNA[21]. The 5¢-arm of the enzyme binds the cleavablesequence via Watson–Crick base pairs and the 3¢-armthrough formation of a DNA triplex. The catalytic residues were derived from the 8–17variant ... activity.When the C deoxyribonucleotide is inserted in the 5¢-T28G29G30-3¢ sequence of PL DNAzyme, the sequence is changed to 5¢-TCGG-3¢, which is the sameas the bulge sequence of 8–17 variant DNAzyme. The stem...

Tài liệu Báo cáo khoa học: Structural features of proinsulin C-peptide oligomeric and amyloid states pptx

... agreement with the results of the NMR. The addition of SDS to the C-peptide sample leads toformation of larger objects, even at an SDS concentra-tion of only 500 lm, which is well below the CMC. The ... about the calcu-lated population distributions cannot be made. The DLS experiments, however, do conﬁrm the formationof C-peptide oligomers in the presence of SDS. Theyalso conﬁrm that the distribution ... 4A, inset). To determine whether the charges in the peptide were relevant, the same experiments werecarried out at pH 3.2 (below the theoretical isoelectricpoint of the peptide; Fig. 4A). At...

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