Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by ﬂuorescence correlation spectroscopy and ﬂuorescence recovery after photobleaching doc

Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc

... IPU consists of a signal sequence (residues1–19) and a mature part (residues 20–564), and the model iscomposed of residues 25–540. The overall structure of the model of IPU and the mutated residues ... polysaccharide,pullulan.Comparison of the active sites of the model of IPU and Dex49A The active site structures of t he model of IPU (Fig. 4A) and Dex49A (Fig. 4B) were compared. The three Asp residues,Asp353, Asp372 and Asp373, ... enzyme by using N MR spectroscopy [17].To compar e the mechanism of h ydrolysis of IPU and o therGH family 49 dextranases, the a nomer conﬁguration of the hydrolysate of IPU was determined....

Tài liệu Báo cáo khoa học: "Methods for the Qualitative Evaluation of Lexical Association Measures" doc

... (Figures 3 and 4), we find: (i) Examination of 50% of the datain the SLs leads to identification of between 75%(AdjN) and 80% (PNV) of the TPs. (ii) For the first 40% of the SLs, and lead to the worstresults, ... fre-quency) and the weak measures ( and ).In the second half of the data in the SLs, how-ever, there is virtually no difference between ,, and the other measures, with the exception of mere co-occurrence ... above50% for the first 10% of the list, but is outper-formed by the t-test afterwards. Looking at the first 40% of the data, there is a big gap between the good measures (t-test, log-likelihood, and fre-quency)...

Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt

... beam of electrons having thermal or near- thermal energies.Capture of a thermal electron by a protonated peptideor protein is exothermic by $ 6 eV and causes the pep-tide backbone to fragment by ... modiﬁed and unmodiﬁed versions of the L34 protein are present ina ratio of 1 : 20. Analysis of the spectra recorded on the other peaksin the base peak ion chromatogram allowed us todetect and ... complementary pair of fragment ions of type c¢ and z¢Æ. Subtraction of the m ⁄ z values for the frag-ments within a given ion series that differ by a singleamino acid affords the mass and thus the identity...

Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by ﬂuorescence correlation spectroscopy and ﬂuorescence recovery after photobleaching doc

... Analysis of the molecular dynamics of medaka nuage proteins by ﬂuorescence correlation spectroscopy and ﬂuorescence recovery after photobleaching Issei Nagao1,*, ... mea-sure the mobility of the components in the PGCs. The medaka embryo was peeled off the chorion, and the segment containing the part of PGCs was excised forobservation by microscopy and FCS ... LSM analysis of Olvas–GFP reveals the dynamicnature of the nuage. Schematic diagram of the preparation of PGCs of medaka specimen (A). Olvas–GFP was expressed in the medaka PGC at stage 24 and...

Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt

... distribution of the size of the inclusions wasnoticed. Moreover, the percentage of cells presentinginclusions as well as the distribution of the size of the inclusions were dependent on when the analysis ... The percentage of the cell population with an FL2-H ﬂuorescence greater than 2 · 101was recorded during the FACS analysis. The percentage of decrease of DYm was calculated as the ratio of the ... cells [39]. Immunoblot analysis of the intracellular level of Hsps revealed anapparent large increase in the level of Hdj-1 and Hdj-2, whereas the upregulation of Hsp27 and Hsp70 levelswas more...

Tài liệu Báo cáo khoa học: "Analysis and Repair of Name Tagger Errors" pptx

... (increasing the F measure by 7.6% over the monocase score). We can also see that the best of the top N (N <= 30) hypotheses is very good, so reranking a small number of hypotheses has the potential ... (typically, the main verb or nominalization representing the event) and a set of arguments. When a name candidate is in-volved in an event, the trigger word and other arguments of the event ... results too, such as the family name and given name of person, the prefixes of the abbreviation names, the modifiers and suffixes of organization names. Then the results from subsequent components...

Tài liệu Báo cáo khoa học: "ANALYSIS OF OONOUNCTIONS IN PAKSER" potx

... instance, the sentence : (2) John saw the boy you told the story and the girl you met yesterday After the analysis of the fragment ending wir/n "story", we get the tree of fig.3.a (and ... a syntactic point of view (SY~IC RULES 2 in fig.l). One of these rules specifies that the subject and the verb of the sentence must agree in nun~r. On the other hand, the subject can be a ... ORGANIZATION OF THE PARSER In this section we overview the principles that lie at the root of the syntactic analysis in FIDO. We try to focus the discussion on the issues that guided the design of the...

Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf

... bc1sub-complex, but the presence of only one of these two subunits does not substitutefor the other. After the addition of ISP, the binding of the last subunit (i.e. Qcr10p) finally occurs. These find-ings ... this molecular mass change is toosmall to explain the dimerization of the bc1complex atthis stage (i.e. just after the addition of ISP and Qcr10p).On the other hand, in the transition from the ... alsounknown. Furthermore, as in the case of the biogene-sis of other multi-subunit complexes of the mitochon-drial respiratory chain, the assistance of specificchaperone proteins is also required. The...

Tài liệu Báo cáo khoa học: Looking for the ancestry of the heavy-chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 a-amylase family ppt

... is the lack of domain B protruding out of the TIM barrel in the place of loop 3 connecting the b3-strand to the a3-helix [9,21]. Sharing domain B by rBATs and GH13 enzymes, and especially the ... subfamilytogether with the intermediary enzymes (Fig. 1). The overall arrangement of the tree is that the clusters of true rBAT and 4F2hc proteins are separated from eachother by the GH13 enzymes.All ... traced in the sequences of hcHAT1 and hcHAT2 groups as well as of the ATG proteins (Fig. 2), indicating evolutionary relationships of allthese enzymes and proteins and hinting at their even-tual...

Tài liệu Báo cáo khoa học: Acetylcholinesterase from the invertebrate Ciona intestinalis is capable of assembling into asymmetric forms when co-expressed with vertebrate collagenic tail peptide doc

... iden-tify the nature of the enzyme and provide additionalinformation about the evolution of the ChEs, including the origins of the acyl pocket, the T exon, and the asymmetric forms of ChE in the vertebrates.Results The ... illustrates the differences between the construction of the acyl pocket in vertebrate and invertebrate AChEs. The line separates the vertebrate and invertebrate AChEs. The numbers at the top of the ﬁgure ... production of the A12asymmetric form of AChE. These results conﬁrmour molecular modeling, which indicated that the appropriate interactions between the WAT domain of the catalytic subunit and the...

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