Tài liệu Báo cáo khoa học: Pharmacologic chaperoning as a strategy to treat Gaucher disease ppt

... administrationincreased Q279E a- galactosidase A residual activity inpatient derived cells; thus, galactose was demonstrated to be ﬁrst active-site-directed pharmacologic chaperonefor a lysosomal storage disease. ... MINIREVIEW Pharmacologic chaperoning as a strategy to treat Gaucher disease Zhanqian Yu, Anu R. Sawkar and Jeffery W. KellyDepartment of Chemistry and The Skaggs Institute for Chemical Biology, ... a- galactosidase A variants (causing anotherlysosomal storage disease) were shown to be foldingand trafﬁcking mutants [16] before this was explored as a possibility in GD. Galactose administrationincreased...

Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc

... CP-pyk(5¢-ACGACTAGTGGATCCATNNNNNAGTTTATTCTTGACANNNNNNNNNNNNNNTGRTATAATNNNNAAGTAATAAAATATTCGGAGGAATTTTGAAATGAATAAACGTGTAAAAATCG-3¢)(N¼ A, T, G, C) and pyk-back (5¢-CTCTACATGCATTTCAACAATAGGGCCTGTC-3¢) for amplification of pyk. The resulting ... (5¢-GGAAGGATCCTTGTGTTTTTCTCCTATAATG-3¢) anddownstream to pyk using primer pyk3 (5¢-GGAAGGATCCTTTGTCAATTAATGATCTTAAAAC-3¢) and pyk4(5¢-CTAGTCTAGATGAGCTCCAGAAGCTTCC-3¢) wereamplified. The PCR products ... À a lasÞÃð1 À eÀ6 a 2:1lasÞÀ33:2 (User defined),Jlactate a lasÞ¼0:919 Ãð129 À a lasÞÃð1 À eÀ6 a 2:1lasÞÀ75:2 (Us erdefined), Jacetate a lasÞ¼0:1135 ÃðÀ30:3 À a lasÞÃð1 À eÀ6 a 3:3lasÞþ4:66...

Tài liệu Báo cáo khoa học: Fermentative lifestyle in yeasts belonging to the Saccharomyces complex ppt

... newgenera Lachancea, Nakaseomyces, Naumovia, Vanderw-altozyma and Zygotorulaspora. FEMS Yeast Res 4,233–245.29 Wolfe KH & Shields DC (1997) Molecular evidencefor an ancient duplication ... kluyveri (Table 1) [36]. A similar situation was detec-ted in species belonging to the Hanseniaspora genus.Hanseniaspora vinae and Hanseniaspora occidentalis didin fact exhibit the ability to produce ... duplication took place after the separationof Saccharomyces, Kazachstania, Naumovia, Nakesimiaand Tetrapisispora from the rest of Saccharomycescomplex genera (Fig. 1).Another problem for comparative...

Tài liệu Báo cáo khoa học: Phage-display as a tool for quantifying protein stability determinants pptx

... stabilities. Remarkably, wehave found that, at least in some circumstances, a quanti-tative correlation to biophysical data can be obtained from a statistical analysis of selected phage populations ... the association of the variable heavy chain (VH)withprotein A was used as a surrogate for direct stabilitymeasurements. The VHdomains in camelid heavy chainantibodies are most similar to ... San Francisco, CA, USA To address questions of protein stability, researchers haveincreasingly turned to combinatorial approaches that permitthe rapid analysis of libraries of protein variants....

Tài liệu Báo cáo khoa học: "Sentence generation as a planning problem" pptx

... to be false. Actions have a number of param-eters, as well as a precondition and effect, both ofwhich are logical formulas. When a planner tries to apply an action, it will ﬁrst create an action ... communicative goals as ﬂaws that the plan must remedy. To this end,we add an atom cg(P ,a 1, ,a n) for each elementP (a 1, ,a n) of the communicative goal to the ini-tial state, and we add a corresponding ... withoutbeing changed by the action. Atoms are printed inboldface iff they contradict the goal.This plan can be read as a derivation tree that hasone node for each action instance in the plan, and anedge...

Tài liệu Báo cáo khoa học: Infrared spectroscopy as a tool for discrimination between sensitive and multiresistant K562 cells doc

... The data were analysed with a window of ﬁvewavenumbers, assuming that adjacent wavenumbers arehighly corr elated. A population o f 3 2 solutions was built ateach generation, and e valuated. ... cells a d ecreaseof the absorptions assigned to fatty a cids and phospholipidsrelative to their protein content. This qua ntitative changewas accompanied by qualitative modiﬁcation, as themethyl/methylene ... greatest interclass variance andthe smallest intraclass variance, and constructs a linearcombination of the variables to discriminate between theclasses. The rule i s constructed with training...

Tài liệu Báo cáo khoa học: "Contrastive accent in a data-to-speech system" doc

... generation of contrastive accent and propose an alternative method that is feasible and computationally at- tractive in data -to- speech systems. 1 Motivation The placement of pitch accent plays ... should have a contrastive accent, because the two teams Ajax and PSV are obviously in each other's alternative set. In fact, though, there is no contrast and PSV should be normally deaccented ... generated sentences. The fact that the input data structures of the system are organized in such a way that identical data types express semantically parallel information allows us to make...

Tài liệu Báo cáo khoa học: Upregulation of the a-secretase ADAM10 – risk or reason for hope? docx

... members of the ADAM family have been shown to act as a- secretase [8,36,37]: ADAM9, ADAM10 andADAM17 (TACE). Overexpression of ADAM9 hasbeen reported to increase the basal and protein kinaseC (PKC) ... mecha-nism: ADAM9 has been shown to proteolytically pro-cess ADAM10 [40–42]. By contrast to ADAM9,ADAM10 was found to have constitutive and regu-lated a- secretase activity as well as many other ... to consider its therapeutic potential in variousdiseases such as cancer or AD. ADAM10 has beenshown to cleave tumour-associated substrates such as MICA [77] or C4. 4A [59] and to be linked to...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: a structure–function perspective of the MLL1 protein ppt

... Milne TA, CopelandTD, Levine SS, Lee JC, Hayes DN, Shanmugam KS,Bhattacharjee A, Biondi CA et al. (2004) Meninassociates with a trithorax family histone methyltrans-ferase complex and with ... generaltranscriptional co-activators that contain histone andtranscription factor acetylation activities [30]. In addi-tion, CBP contains a number of protein-bindingdomains that mediate transcription ... CBP is a structural platform that is capable of binding severaldifferent families of transcriptional activators [30], andevidence indicates that the KIX domain has the ability to simultaneously...

Tài liệu Báo cáo khoa học: Bacitracin is not a speciﬁc inhibitor of protein disulﬁde isomerase pptx

... rhodanesewas also decreased by bacitracin. The decrease in thenoncatalyzed rate (31%) was similar to that of thePDI-catalyzed reaction (a 32% decrease). These resultsFig. 1. Bacitracin has minimal ... showing a greater effect(29% decrease in aggregation rate) than PDI (18%decrease in rate). When 1mm bacitracin was added to the PDI-catalyzed reaction the rate of aggregation ofrhodanese was signiﬁcantly ... Escherichia coli DsbA and DsbC, as well as the isolated catalytic a domain of PDI. Boththe PDI a domain and DsbA have a catalytic site, withan associated substrate-binding site, but lack an inde-pendent...

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